Akkermansia muciniphila reduces Porphyromonas gingivalis‐induced inflammation and periodontal bone destruction

Abstract

Aim: Akkermansia muciniphila is a beneficial gut commensal, whose anti-inflammatory properties have recently been demonstrated. This study aimed to evaluate the effect of A. muciniphila on Porphyromonas gingivalis elicited inflammation. Material and Methods:In lean and obese mice, A. muciniphila was administered in P. gingivalis-induced calvarial abscess and in experimental periodontitis model (EIP).Bone destruction and inflammation were evaluated by histomorphometric analysis.In vitro, A. muciniphila was co-cultured with P. gingivalis, growth and virulence factor expression was evaluated. Bone marrow macrophages (BMMϕ) and gingival epithelial cells (TIGK) were exposed to both bacterial strains, and the expression of inflammatory mediators, as well as tight junction markers, was analysed. Results: In a model of calvarial infection, A. muciniphila decreased inflammatory cell infiltration and bone destruction. In EIP, treatment with A. muciniphila resulted in a decreased alveolar bone loss. In vitro, the addition of A. muciniphila to P. gingivalisinfected BMMϕ increased anti-inflammatory IL-10 and decreased IL-12. Additionally, A. muciniphila exposure increases the expression of junctional integrity markers such as integrin-β1, E-cadherin and ZO-1 in TIGK cells. A. muciniphila co-culture with P. gingivalis reduced gingipains mRNA expression. Discussion: This study demonstrated the protective effects of A. muciniphila administration and may open consideration to its use as an adjunctive therapeutic agent to periodontal treatment. K E Y W O R D S infection, inflammation, periodontitis, probiotic | 203 HUCK et al.

The Bacterium Akkermansia muciniphila: A Sentinel for Gut Permeability and Its Relevance to HIV-Related Inflammation

“…Other studies have also shown the anti-inflammatory properties of A. muciniphila in different mouse models including germfree, liver injury and obesity models (59-64). Huck et al (62) reported that A. muciniphila could reduce inflammation induced by Porphyromonas gingivalis in lean or obese mice. Ansaldoi et al (59) demonstrated that A. muciniphila plays a contextdependent role in the induction of gut-resident T-cells during homeostasis in mice.…”

Quercetin inhibits virulence properties of Porphyromas gingivalis in periodontal disease

“…P. gingivalis which detected with greater frequency and at higher levels at periodontal sites is strongly associated with periodontal disease and has been considered as a major etiologic agent for periodontitis 22 , 23 . In the present study, we determined the effect of quercetin on gingipain activities, hemagglutination activity, hemolytic activity, biofilm formation, hydrophobicity and aggregation, which all contribute to P. gingivalis virulence factors.…”

Effect of Periodontal Disease on Diabetic Retinopathy in Type 2 Diabetic Patients: A Cross-Sectional Pilot Study

“…The involvement of reactive oxygen species (ROS) is considered to be a factor in which inflammation of the gingiva affected diabetic retinopathy [ 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 ]. Porphyromonas gingivalis ( P. gingivalis ) and Fusobacterium nucleatum ( F .…”

“…Porphyromonas gingivalis ( P. gingivalis ) and Fusobacterium nucleatum ( F . nucleatum ) are the major periodontal bacteria that cause gingival inflammation [ 26 , 27 ]. It has been reported that diabetic model rats infected with P .…”