ACTINOBACILLUS PLEUROPNEUMONIAESEROPREVALENCE IN FARMED WILD BOARS IN FINLAND

Abstract

The aim of this study was to estimate the prevalence of serum antibodies to Actinobacillus pleuropneumoniae (APP) in Finnish farmed wild boars and to evaluate basic risk factors for seropositivity of the agent. A sampling frame was compiled based on the national record of wild boar farmers (n = 117), and 26 herds were included in the study. Serum samples (n = 206) were obtained from all animals slaughtered from these herds during the 2007-2008 period. Indirect enzyme-linked immunosorbent assay techniques were used to detect the possible presence of antibodies toward 12 serotypes of APP. Altogether, 1,442 analyses were performed, out of which 35 (2.4%) exceeded the positive cut-off value. Correspondingly, 26 of the pigs (12.6%) were found to be seropositive for any serotype of APP. The apparent animal prevalence of the cross-reacting APP serotypes 1, 9, and 11 was 3.9% (95% confidence interval [CI] 1-7%); it was 0.5% (CI 0-1%) for serotype 2; 3.4% (CI 1-6%) for serotypes 3, 6, and 8; 7.3% (CI 4-11%) for serotypes 4 and 7; 0.5% (CI 0-1%) for serotype 10; and 1.5% (CI 0-3%) for serotype 12. No antibodies against serotype 5s were detected. The apparent farm prevalence of APP serotypes 1,9, and 11 was 15.4% (CI 2-29%); that of serotype 2 was 3.8% (CI 0-11%); that of serotypes 3, 6, and 8 was 11.5% (CI 0-24%); that of serotypes 4 and 7 was 34.6% (CI 16-53%); that of serotype 10 was 3.8% (CI 0-11%); and that of serotype 12 was 7.7% (CI 0-18%). In conclusion, the prevalence of serum antibodies to different APP serotypes was low in farmed wild boar. Risk factor analysis did not reveal any significant animal- or herd-level risk factors for seropositivity of the agent.

Bacterial Pneumonia in Captive Wild Boars in Southern Brazil - Etiological and Pathological Causes

“…In Germany, A. pleuropneumoniae infection status was evaluated and gross pathologic lesions typical for pleuropneumonia were not detected, even though 35.8% of the wild boars were PCR positive [35]. Furthermore, the detection of serum antibodies against A. pleuropneumoniae has been described in finishing captive wild boars [13], suggesting that the role of A. pleuropneumoniae and G. parasuis in pneumonia of captive wild boars needs to be better evaluated.…”

iTRAQ-based quantitative proteomic analysis of peripheral blood serum in piglets infected with Actinobacillus pleuropneumoniae

“…Actinobacillus Pleuropneumoniae serovar 5b reference strain L20 (courtesy of Shanghai entry-exit inspection and quarantine bureau) was cultured and prepared for experimental intranasal infection studies as described previously (Bao et al 2019). In brief, piglets were inoculated intranasally with L20 (wild type, WT) with 3.1 × 1010 CFU (Halli et al 2014;Jiang et al 2018). Nine, 5-week-old, APP free Landrace healthy piglets (approximately 10 kg body weight) were purchased from the animal breeding center of Jilin University.…”

“…The piglets were housed in the animal room of Jilin University Veterinary Medicine School. The piglets were healthy and free from APP, as adjudged by ELISA using APP bacterial lysates as coated antigen (Halli et al 2014). All treatments of the piglets were carried out by following animal experiments protocols approved by the State Council of People's Republic of China.…”

Transcript profiling of the immunological interactions between Actinobacillus pleuropneumoniae serotype 7 and the host by dual RNA-seq

“…Fifteen serotypes of App have been detected, and these can cause acute hemorrhagic pneumonia and chronic interstitial pneumonia and have markedly affected the porcine industry [ 1 ]. Even with the optimization of intensive and large-scale breeding patterns and the development of vaccines for the disease, many districts have observed high morbidity rates due to App in recent years [ 2 – 4 ]. This disease is airborne, and the wild strains can easily break through the defense of a sound vaccine prevention and control system, resulting in rapid proliferation.…”