Hypoxia and collagen crosslinking in the healthy and affected sites of periodontitis patients

Taşkan, Mehmet Murat; Karataş, Özkan; Yüce, Hatice Balcı; Kara, Gozde Isiker; Gevrek, Fıkret; Yarkaç, Fatma Uçan

doi:10.1080/00016357.2019.1624819

Cited by 7 publications

(4 citation statements)

References 44 publications

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“…The expression of VEGF is associated with metabolic syndrome or its components [ 44 ]. Recently, some studies have reported that VEGF expression can be detected in periodontitis [ 17 , 30 , 31 ]. However, the results of VEGF expression in periodontitis and controls were still inconsistent [ 16 , 17 ].…”

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: VEGF as a potential molecular target in periodontitis: a meta-analysis and microarray data validation

Ren

Feng

et al. 2021

J Inflamm

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Background Anti-vascular endothelial growth factor (VEGF) has been used as a therapeutic drug for the treatment of some human diseases. However, no systematic evidence is performed for assessing the role of VEGF in periodontitis. We carried out a comprehensive analysis to explore the role of VEGF in patients with periodontitis. Methods Multiple databases were searched for eligible studies. The pooled standardized mean difference (SMD) and odds ratio (OR) with the corresponding 95% confidence interval (CI) were applied to evaluate the effect sizes. Clinical data validation from microarray analysis was used. Pathway and process enrichment analysis were also investigated. Results Finally, 16 studies were included in this analysis. Overall, there was a significantly higher level of VEGF expression in periodontitis than in healthy control groups (OR = 16.64, 95% CI = 6.01–46.06, P < 0.001; SMD = 2.25, 95% CI = 1.25–3.24, P < 0.001). Subgroup analysis of ethnicity showed that VEGF expression was still correlated with periodontitis in the Asian and European populations. No correlation was observed between VEGF expression and age, gender, and pathological type. A large clinical sample data (427 periodontitis patients and 136 healthy controls) further validated that VEGF expression was higher in periodontitis than in healthy control groups (P = 0.023). VEGF was involved in many functions such as blood vessel development, response to growth factor, cell proliferation, and cell adhesion. Conclusions High levels of VEGF were credible implications for the development of periodontitis. Anti-VEGF therapy may be valuable for the treatment of periodontitis in clinical management.

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Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: VEGF as a potential molecular target in periodontitis: a meta-analysis and microarray data validation

Ren

Feng

et al. 2021

J Inflamm

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“…The effect of LPS-G stimulation may induce an inflammatory response in hGMSCs that could reproduce similarly the periodontitis microenvironment [ 34 ]. The most studied complex involved in intracellular inflammation is the NLRP3 inflammasome, which can be activated when cells are exposed to a wide range of bacterial ligands, including LPS-G [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Antioxidant Ascorbic Acid Modulates NLRP3 Inflammasome in LPS-G Treated Oral Stem Cells through NFκB/Caspase-1/IL-1β Pathway

et al. 2021

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Human gingival mesenchymal stem cells (hGMSCs) and endothelial committed hGMSCs (e-hGMSCs) have considerable potential to serve as an in vitro model to replicate the inflammation sustained by Porphyromonas gingivalis in periodontal and cardiovascular diseases. The present study aimed to investigate the effect of ascorbic acid (AA) on the inflammatory reverting action of lipopolysaccharide (LPS-G) on the cell metabolic activity, inflammation pathway and reactive oxygen species (ROS) generation in hGMSCs and e-hGMSCs. Cells were treated with LPS-G (5 μg mL−1) or AA (50 μg mL−1) and analyzed by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay, immunofluorescence and Western blot methods. The rate of cell metabolic activity was decreased significantly in LPS-G-treated groups, while groups co-treated with LPS-G and AA showed a logarithmic cell metabolic activity rate similar to untreated cells. AA treatment attenuated the inflammatory effect of LPS-G by reducing the expression of TLR4/MyD88/NFκB/NLRP3/Caspase-1/IL-1β, as demonstrated by Western blot analysis and immunofluorescence acquisition. LPS-G-induced cells displayed an increase in ROS production, while AA co-treated cells showed a protective effect. In summary, our work suggests that AA attenuated LPS-G-mediated inflammation and ROS generation in hGMSCs and e-hGMSCs via suppressing the NFκB/Caspase-1/IL-1β pathway. These findings indicate that AA may be considered as a potential factor involved in the modulation of the inflammatory pathway triggered by LPS-G in an vitro cellular model.

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“…Xie (Xie et al, 2018) reported that LOXs in the meniscus were reduced at an early stage after anterior cruciate ligament transection surgery. LOX expression was decreased in the tissue of periodontitis patients (Taskan et al, 2019). In other studies, the gene expression of LOX in oral epithelial cells was increased after exposure to Fusobacterium nucleatum but decreased after exposure to Porphyromonas gingivalis (Milward et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Berberine regulates bone metabolism in apical periodontitis by remodelling the extracellular matrix

et al. 2021

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Objectives:The objectives of this study were to explore the role and related mechanism of berberine in repairing bone destruction in apical periodontics (AP).Materials and Methods: AP was established in 14 of 21 male Wistar rats (four weeks of age; 70-80 g) for 3 weeks. The canals were cleaned and administered berberine (2 mg/ ml; n = 7) or calcium hydroxide (100 mg/ml; control; n = 7), followed by glass ionomer cement sealing. After 3 weeks, specimen collection followed by micro-computed tomography (μ-CT) and histological staining was performed, including haematoxylin and eosin staining, Masson's trichrome staining, tartrate-resistant acid phosphatase staining, immunohistochemistry and immunofluorescence histochemistry.Results: μ-CT showed that AP lesion volume reduced in the berberine group.Histopathology showed that berberine decreased the activity and number of osteoclasts but increased the expression of proteins related to osteoblast differentiation, including alkaline phosphatase and osterix. The immune cell, T cell, dendritic cell and macrophage counts were significantly decreased in the berberine group. In the berberine group, the expression of extracellular matrix-degraded proteases, metalloproteinases, was decreased; however, that of extracellular matrix-stable proteases, lysyl oxidases, was increased.Conclusions: Berberine controlled the inflammatory response and regulated bone metabolism in AP by reducing metalloproteinase expression and increasing lysyl oxidases expression.

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Hypoxia and collagen crosslinking in the healthy and affected sites of periodontitis patients

Cited by 7 publications

References 44 publications

RETRACTED ARTICLE: VEGF as a potential molecular target in periodontitis: a meta-analysis and microarray data validation

RETRACTED ARTICLE: VEGF as a potential molecular target in periodontitis: a meta-analysis and microarray data validation

Antioxidant Ascorbic Acid Modulates NLRP3 Inflammasome in LPS-G Treated Oral Stem Cells through NFκB/Caspase-1/IL-1β Pathway

Berberine regulates bone metabolism in apical periodontitis by remodelling the extracellular matrix

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