Hypoglycemic mechanism of polysaccharide from Cyclocarya paliurus leaves in type 2 diabetic rats by gut microbiota and host metabolism alteration

Li, Qiqiong; Hu, Jielun; Nie, Qixing; Chang, Xiao; Fang, Qin; Xie, Junhua; Li, Haishan; Nie, Shaoping

doi:10.1007/s11427-019-1647-6

Cited by 50 publications

(37 citation statements)

References 47 publications

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“…Lactobacillus is closely linked with bile acid metabolism and plays an important role in the absorption of dietary fat and vitamins [42]. In addition, it is reported that Christensenellaceae_R-7_group and Ruminococcaceae_UCG-005 are related to the production of short-chain fatty acids [40,43]. Christensenellaceae_R-7_group can produce butyric acid, and butyric acid contributes to the relative mRNA expression and secretion of Mucin 2 [40].…”

Section: Discussionmentioning

confidence: 99%

“…Christensenellaceae_R-7_group can produce butyric acid, and butyric acid contributes to the relative mRNA expression and secretion of Mucin 2 [40]. Ruminococcaceae_UCG-005 is thought to be a producer of acetic acid and butyric acid, and helps regulate hormone levels and inflammation [43]. Although the animals were normally healthy, environmental stimuli, such as nightlight, or the potential for disease, can also cause a stress response or differences in their hormone levels that might affect the structure of the gut microbiota, which were not monitored in this study.…”

Section: Discussionmentioning

confidence: 99%

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Sex Differences in Intestinal Microbial Composition and Function of Hainan Special Wild Boar

Wang

Zhang

Qin

et al. 2020

Animals

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The gut microbiome plays an important role in the health and disease status of the host. Research on the effect of sex on animal intestinal microorganisms is still limited; and the effect of castration on the gut microbiome of male pigs has not been fully investigated. In this study, 30 Hainan special wild boars at the same growth stage were divided into three groups (10 entire males, 10 females, and 10 castrated males). High-throughput 16S rRNA sequencing was used to investigate the fecal microbiota of the Hainan special wild boar. Firmicutes, Bacteroidetes, Actinobacteria, Spirochaetes, and Proteobacteria were the five dominant phyla found in the specimens. The relative abundance of Bacteroidetes was higher in the microbiota of female pigs than in male pigs, while Firmicutes was on the contrary. The percentage of Streptococcus and Lactobacillus was higher in males than females. The microbial diversity of females was significantly higher compared to males; castration increased the intestinal microbial diversity of males. Functional prediction showed that male fecal microorganisms were rich in membrane transport and carbohydrate metabolism; energy metabolism, glycan biosynthesis, and metabolism of cofactors and vitamins were rich in the female group; the fecal microorganisms of castrated males had higher membrane transport abundance.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Sex Differences in Intestinal Microbial Composition and Function of Hainan Special Wild Boar

Wang

Zhang

Qin

et al. 2020

Animals

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“…These isolates showed various pharmacological activities including anti-inflammation, anti-oxidation, anti-microbial, anti-cancer, and anti-diabetes activities [47][48][49]. Different extracts from C. paliurus were reported with anti-diabetic potential on different animal models [17,23,24,48,50]. Until now, no random-controlled clinical trial of C. paliurus has been reported.…”

Section: Discussionmentioning

confidence: 99%

“…These results indicate that CPF and CPP possess anti-inflammation effect on STZ-induced mice. Inflammation can be triggered by structural components of gut bacteria, resulting in a cascade of inflammatory pathways involving interleukins and other cytokines [24]. Previous studies showed that pro-inflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were associated with microbiota imbalance in diabetic mice [25,26].…”

Section: Cpf and Cpp Suppressed Inflammatory Cytokine Levels In Stz-induced Diabetic Micementioning

confidence: 99%

Anti-Hyperglycemic Effects of Refined Fractions from Cyclocarya paliurus Leaves on Streptozotocin-Induced Diabetic Mice

et al. 2021

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To identify the chemical components responsible for the anti-hyperglycemic effect of Cyclocarya paliurus (Batal.) Iljinsk (Juglandaceae) leaves, an ethanol extract (CPE) and a water extract (CPW) of C. paliurus leaves, as well as their total flavonoids (CPF), triterpenoids (CPT) and crude polysaccharides (CPP), were prepared and assessed on streptozotocin (STZ)-induced diabetic mice. After being orally administrated once a day for 24 days, CPF (300 mg/kg), CPP (180 mg/kg), or CPF+CPP (300 mg/kg CPF + 180 mg/kg CPP) treatment reversed STZ-induced body weight and muscle mass losses. The glucose tolerance tests and insulin tolerance tests suggested that CPF, CPP, and CPF+CPP showed anti-hyperglycemic effect in STZ-induced diabetic mice. Furthermore, CPF enhances glucose-stimulated insulin secretion in MIN6 cells and insulin-stimulated glucose uptake in C2C12 myotubes. CPF and CPP suppressed inflammatory cytokine levels in STZ-induced diabetic mice. Additionally, CPF and CPP improved STZ-induced diabetic nephropathy assessed by H&E staining, blood urea nitrogen content, and urine creatinine level. The molecular networking and Emperor analysis results indicated that CPF showed potential anti-hyperglycemic effects, and HPLC–MS/MS analysis indicated that CPF contains 3 phenolic acids and 9 flavonoids. In contrast, CPT (650 mg/kg) and CPC (300 mg/kg CPF + 180 mg/kg CPP + 650 mg/kg CPT) did not show anti-hyperglycemic effect. Taken together, polysaccharides and flavonoids are responsible for the anti-hyperglycemic effect of C. paliurus leaves, and the clinical application of C. paliurus need to be refined.

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“…Then, diabetes was induced in high‐fat diet‐fed mice with a single intraperitoneal injection of streptozotocin (STZ; Sigma) at a dose of 35 mg/kg bodyweight according to the previous reports to mimic the GDM status (plasma glucose levels > 11 mmol/L) (Wang, 2013; Zeng et al., 2017). For LP intervention, LP was added into diet at 1% (w/w) according to previous research (Li et al., 2012, 2020; Wang, 2013; Zeng et al., 2017). The maternal mice were housed one per cage at room temperature (25±2°C) and moderate humidity (50±10%) with a 12/12 h of light/dark cycle till spontaneous delivery.…”

Section: Methodsmentioning

confidence: 99%

Polysaccharides isolated from Laminaria japonica attenuates gestational diabetes mellitus by regulating the gut microbiota in mice

Lin

Zhang

et al. 2021

Food Frontiers

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The current study was aimed to explore the beneficial effects of polysaccharides isolated from Laminaria japonica (LP) on gestational diabetes mellitus (GDM) mice. The obtained results demonstrated that the LP improved serum biochemical index, body weight index, and glucose tolerance. Furthermore, the evidence also suggested that the beneficial effects of LP might be attributed to the alternation in gut microbiota by LP supplementation. Particularly, the Turicibacter, a short‐chain fatty acids‐producing bacterium, was found to be up‐regulated. In conclusion, the obtained results indicated that the LP might serve as prebiotic, and might have a great potential to be used as food supplementation for GDM patients.

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Hypoglycemic mechanism of polysaccharide from Cyclocarya paliurus leaves in type 2 diabetic rats by gut microbiota and host metabolism alteration

Cited by 50 publications

References 47 publications

Sex Differences in Intestinal Microbial Composition and Function of Hainan Special Wild Boar

Sex Differences in Intestinal Microbial Composition and Function of Hainan Special Wild Boar

Anti-Hyperglycemic Effects of Refined Fractions from Cyclocarya paliurus Leaves on Streptozotocin-Induced Diabetic Mice

Polysaccharides isolated from Laminaria japonica attenuates gestational diabetes mellitus by regulating the gut microbiota in mice

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