Hypertonic saline and dextran in normovolaemic and hypovolaemic healthy volunteers increases interstitial and intravascular fluid volumes

Töllöfsrud, Stein; Tønnessen, Theis; Skraastad, Øyvind; Noddeland, Harald

doi:10.1111/j.1399-6576.1998.tb05100.x

Cited by 53 publications

(65 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Likewise, it was demonstrated that subcutaneous injections of HS result in robust water drinking by mice (22). In addition, increased diuresis was reported after the administration of hypertonic solutions (28). Therefore, in the HS group, a higher consumption of DSS provided through the drinking water may have occurred.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Hypertonic saline reduces neutrophil-epithelial interactions in vitro and gut tissue damage in a mouse model of colitis

Tillinger¹,

McCole²,

Keely³

et al. 2008

American Journal of Physiology-Regulatory, Integrative and Comp

View full text Add to dashboard Cite

Hypertonic saline (HS) exerts various inhibitory effects on PMN function. We hypothesized that HS could inhibit transepithelial migration of PMN and thereby prevent inflammatory events in experimental colitis. Isolated human PMN were treated with HS (40 mM), and their transmigration across a monolayer of T84 epithelial cells was induced by N-formyl-methionyl-leucyl-phenylalanine. Monolayer disruption was assessed by monitoring changes in transepithelial conductance in an Ussing chamber. Colitis in mice was induced by oral administration of dextran sulfate sodium (DSS). Animals were treated with 4 or 8 ml/kg of 7.5% saline intraperitoneally two times daily for 7 days. Controls received equivalent volumes of normal saline (NS, n ϭ 6) or no intraperitoneal treatment (DSS, n ϭ 12). The severity of inflammation was evaluated based on disease activity index and histology score. HS treatment of PMN in vitro significantly reduced cell migration and the disruption of T84 monolayers compared with untreated control cells (n ϭ 5, P Ͻ 0.05). This effect of HS was dose dependent. HS treatment in vivo also reduced colitis-induced gut tissue damage, as indicated by an improved histology score compared with the NS and DSS groups. We conclude that HS inhibits transepithelial migration of PMN in vitro and gut tissue damage in vivo in a mouse model of colitis. Thus HS may have clinical value to reduce PMN-mediated intestinal damage. neutrophil activation; inflammatory bowel disease; hypertonic saline solution; inflammation; epithelium ALTHOUGH INFLAMMATORY DISEASES of the intestine are etiologically diverse, many of these diseases express similar histological features. Accumulation of neutrophils [polymorphonuclear neutrophils (PMN)] in the lamina propria is a major hallmark of the acute inflammatory response in disorders ranging from inflammatory bowel disease (IBD) to shock-induced gut dysfunction. In IBD, PMN also transmigrate from the lamina propria in the gut lumen. By measuring fecal excretion of leukocytes labeled with indium-111, migration of PMN from the circulation in the diseased intestine has been estimated to be increased by at least 10-fold in IBD patients (23). PMN are rapidly recruited from the bloodstream by cell adhesion to the vascular endothelium, transendothelial migration, and chemotaxis to the local inflammatory site. After their recruitment to such sites, PMN exert cytotoxic effects that can damage gut tissues via the release of cytotoxic mediators. A complex assortment of these cytotoxic agents, including reactive oxygen metabolites and proteolytic enzymes, is released during this inflammatory process and causes host tissue damage. In addition, PMN can further modulate such inflammatory processes by immunoregulatory functions they exert via the secretion of several important cytokines, including interleukin (IL)-1, IL-1 receptor antagonist, IL-6, IL-8, tumor necrosis factor-␣, granulocyte colony-stimulating factor and granulocyte macrophage colony-stimulating factor (15).The transepithelial migration of PMN...

show abstract

Section: Discussionmentioning

confidence: 99%

“…Recently, there has been renewed interest in the use of hyperosmolar resuscitation fluids in shock therapy. Infusion of hypertonic saline (HS) results in a rapidly increased intravascular blood volume due to osmotically induced fluid shifts from the extravascular compartments to the vascular bed (28).…”

mentioning

confidence: 99%

Hypertonic saline reduces neutrophil-epithelial interactions in vitro and gut tissue damage in a mouse model of colitis

Tillinger¹,

McCole²,

Keely³

et al. 2008

American Journal of Physiology-Regulatory, Integrative and Comp

View full text Add to dashboard Cite

show abstract

“…This leads to a translocation of nearly 4/5 of the administered volume to the interstitium, resulting in edema and tissue hypoxia 4 .…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, at least, three times the amount of blood lost should be replaced with crystaloids³. Of the remainder left in the intravascular, about half is eliminated as urine and the other half will form interstitial edema 4 . As a result, there is a great amount of fluid draining into the extravascular space with formation of interstitial edema.…”

Section: Introductionmentioning

confidence: 99%

Noradrenaline and dobutamine effects on the volume expansion with normal saline in rabbits subjected to hemorrhage

Ramalho

Vane²,

Lima

et al. 2016

Acta Cir. Bras.

View full text Add to dashboard Cite

PURPOSE:To evaluate the effects of dobutamine (DB), noradrenaline (NA), and their combination (NADB), on volume retention in rabbits submitted to hemorrhage. METHODS:Thirty six rabbits were randomly divided into 6 groups: SHAM, Control, Saline, DB, NA, DB+NA. All the animals, except for SHAM, were subjected to hemorrhage of 25% of the calculated blood volume. Control animals were replaced with their own blood. The other groups received NSS 3 times the volume withdrawn. The intravascular retention, hematocrit, diuresis, central venous pressure, mean arterial pressure, NGAL, dry-to-wet lung weight ratio (DTWR) and the lung and kidney histology were analyzed. RESULTS:Replacement with NSS and NA, DB or NA+DB did not produce differences in the intravascular retention. After hemorrhage, the animals presented a significant decrease in the MAP and CVP, which were maintained until volume replacement. Regarding NGAL, dry-to-wet-lung-weight ratio, lung and kidney histology, there were no statistical differences between the groups. CONCLUSION:The use of noradrenaline, dobutamine or their combination did not increase the intravascular retention of volume after normal saline infusion.

show abstract

“…The crystalloids have little effect on volemic expansion because only 20% of the total quantity that is infused remains in the intravascular space 20 minutes after infusion. Half of the infused volume is eliminated as urine, and 30% causes edema [3][4][5][6] .…”

Section: Introductionmentioning

confidence: 99%

Effects of catecholamines on volemic replacement with saline solution and the impact on heart rate variability in rabbits subjected to hemorrhage. A study by spectral analysis

et al. 2014

View full text Add to dashboard Cite

PURPOSE: To verify the effects of different catecholamines on volemic expansion and on the autonomic nervous system in rabbits that were subjected to hemorrhage. METHODS:Twenty four rabbits subjected to hemorrhage (with a 25% loss of blood volume) and were randomly divided into four experimental groups: 1) HEMO Group underwent replacement with their own blood in an equal volume; 2) SS Group underwent replacement with saline solution (SS) in a volume that corresponded to three times the removed blood volume; 3) ISP Group underwent replacement with SS and isoprenaline; 4) FNL Group underwent replacement with SS and phenylephrine. Spectral Analysis of the heart rate and heart rate variability were performed from the recorded data. Hematocrit was measured throughout the experiment. RESULTS:Replacement with SS and an α-or β-agonist did not produce differences in the intravascular retention compared to replacement with SS alone. An analysis of HRV showed that the FNL group maintained the LF/HF ratio better than ISP and SS. CONCLUSIONS:No difference in vascular retention when α-or β-agonists were added to SS during post-hemorrhagic recovery. The animals in the FNL group maintained the integrity of the autonomic response within normal physiological standards during hemorrhagic stress.

show abstract

Hypertonic saline and dextran in normovolaemic and hypovolaemic healthy volunteers increases interstitial and intravascular fluid volumes

Cited by 53 publications

References 16 publications

Hypertonic saline reduces neutrophil-epithelial interactions in vitro and gut tissue damage in a mouse model of colitis

Hypertonic saline reduces neutrophil-epithelial interactions in vitro and gut tissue damage in a mouse model of colitis

Noradrenaline and dobutamine effects on the volume expansion with normal saline in rabbits subjected to hemorrhage

Effects of catecholamines on volemic replacement with saline solution and the impact on heart rate variability in rabbits subjected to hemorrhage. A study by spectral analysis

Contact Info

Product

Resources

About