Hyperhomocysteinemia, Endothelial Nitric Oxide Synthase Polymorphism, and Risk of Coronary Artery Disease

Abstract

Background:Hyperhomocysteinemia is an independent, graded risk factor for coronary artery disease (CAD). The G894T variant of endothelial nitric oxide synthase (eNOS) was postulated to be associated with hyperhomocysteinemia and could influence individual susceptibility to CAD. The aims of this study were to investigate (a) the relationship of the eNOS G894T polymorphism with the presence and the severity of CAD and (b) the possible relationship between hyperhomocysteinemia and the eNOS G894T variant for the risk of CAD severity in a Tunisian population. Methods: We used PCR with restriction fragment length polymorphism analysis to detect the G894T variant of the eNOS gene in 100 patients with CAD and 120 healthy controls. The severity of CAD was expressed by the number of affected vessels. Total plasma homocysteine concentrations were determined by direct chemiluminescence assay. Results: The frequencies of the eNOS GG, GT, and TT genotypes in the CAD group were significantly different from those in the control group (45%, 44%, and 11% vs 60%, 35.8% and 4.2%, respectively; P ‫؍‬ 0.035). There was no association between the eNOS G894T genotype frequencies and the number of stenosed vessels (P ‫؍‬ 0.149). In the CAD group, the coexistence of the 894 GT

Impact of Nitric Oxide Synthase Glu298Asp Polymorphism on the Development of End-Stage Renal Disease in Type 2 Diabetic Egyptian Patients

“…Moreover, it was observed that 5% of control subjects had TT genotype, and 60% of them had GG genotype and this is consistent with the rates established among the Tunisian population. 31,32 These results are in agreement with the findings of Neugebauer et al 21 and Shin Shin et al, 33 who reported an association between the eNOS gene polymorphism and the progression of DN in Japanese and Korean type 2 diabetic patients, respectively. These notions were supported by recent reports.…”

Lack of role of endothelial nitric oxide synthase gene Glu298Asp polymorphism in rheumatoid arthritis among Asian Indians

“…However, in one study in our lab on the same polymorphism GG dominates over GT and TT genotypes [9]. Likewise in several other studies, GG dominates over GT and TT genotypes [10,11,12]. In our study, the distribution pattern did not obey Hardy Weinberg equilibrium.…”

Homocysteine as Predictive Marker for Pregnancy-Induced Hypertension—A Comparative Study of Homocysteine Levels in Normal Versus Patients of PIH and Its Complications

“…More of the complications were due to the direct implication of homocysteine on the endothelial damage. Hyperhomocysteinemia is associated with vascular diseases such as hypertension, cerebrovascular accidents, peripheral vascular diseases, as well as early pregnancy loss and placental abruption [9][10][11][12][13]. Abruption was found in 2 patients in the study conducted by Sunita et al [6].…”