Hygrophorus eburneus, edible mushroom, a promising natural bioactive agent

Kosanić, Marijana; Šeklić, Dragana; Jovanović, Mića; Petrović, Nevena; Marković, Snežana D.

doi:10.17179/excli2019-2056

Cited by 6 publications

(4 citation statements)

References 25 publications

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“…Wound healing (Scratch) assay was performed for assessment of migratory potential of cancer cells 24 hr after treatment with PG, according to protocol already described (Kosanić et al, 2020). Phasecontrast microscope (Delta Optical Genetic PRO microscope) was used at 100× magnification to obtain micrographs, wherein wound space width was measured.…”

Section: Cell Migration Assay (Wound Healing Assay)mentioning

confidence: 99%

Platismatia glauca —Lichen species with suppressive properties on migration and invasiveness of two different colorectal carcinoma cell lines

Šeklić

Jovanović

2022

Journal of Food Biochemistry

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Platismatia glauca is a popular lichen traditionally used as a spice and possesses significant anti-cancer potential, whose anti-migratory/anti-invasive properties were mostly disregarded. Migration/invasion of cancer cells is processed in cancer metastasis and targeting their markers is an important strategy in anti-cancer treatment. We examined the anti-migratory/anti-invasive properties of P. glauca extract on two colorectal carcinoma cell lines (HCT-116 and SW-480) and elucidated possible mechanisms underlying these properties. Cell migration was evaluated by wound healing and RTCA methods. Immunofluorescent assay was used for the analysis of protein, while qRT-PCR for gene expression of migratory/invasive markers. ELISA assay was applied for the determination of MMP-9 concentration. P. glauca extract inhibited the motility of tested cells, by reducing pro-migratory/pro-invasive markers and potentially retaining intercellular connections. Treatment showed cell-selective effects, and HCT-116 cells were more responsive. Our study presents important scientific novelty, thus these lichen properties should be furtherly examined regarding the amelioration of anticancer treatment.

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Section: Cell Migration Assay (Wound Healing Assay)mentioning

confidence: 99%

Platismatia glauca —Lichen species with suppressive properties on migration and invasiveness of two different colorectal carcinoma cell lines

Šeklić

Jovanović

2022

Journal of Food Biochemistry

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“…The results were evaluated 24 and 72 h after treatment, and absorbances were measured on ELISA reader (Elisa Microplate Reader RT‐6100; Rayto). Wound healing assay was used for the determination of antimigratory potential of LSE (concentrations 10 and 50 μg/mL), and results were determined after 24 h. Effects were analyzed on HCT‐116 and HeLa cells, and assay protocol has been already described by Kosanić et al ( 2020 ).…”

Section: Methodsmentioning

confidence: 99%

“…Wound healing assay was used for the determination of antimigratory potential of LSE (concentrations 10 and 50 μg/mL), and results were determined after 24 h. Effects were analyzed on HCT-116 and HeLa cells, and assay protocol has been already described by Kosanić et al (2020).…”

Section: Determination Of Anticancer Activitymentioning

confidence: 99%

Anticancer assessment and antibiofilm potential of Laetiporus sulphureus mushroom originated from Serbia

Jovanović,

Marković,

Grujović

et al. 2023

Food Science & Nutrition

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Laetiporus sulphureus (Bull.) Murrill is a well‐known edible mushroom consumed in nutrition as delicacy. It has been used in traditional medicine because of its beneficial effects on human wellness, such as antimicrobial, antioxidant, and anticancer potential. The present study determined the phenolic profile of Laetiporus sulphureus ethanolic extract (LSE) by high‐performance liquid chromatographic method. Tolerance of two probiotic bacterial strains Lactiplantibacillus plantarum 229v, Bifidobacterium animalis subsp. lactis and probiotic yeast Saccharomyces boulardii on LSE was analyzed in terms of viability and biofilm formation. Effects of extract on colorectal (HCT‐116) and cervical (HeLa) cancer cells viability was determined using MTT test in concentration range: 1–500 μg/mL after 24 and 72 h. Redox parameters (superoxide anion radicals, nitrites, and reduced glutathione) were evaluated using NBT, Griess, and GSH assays in the concentration range of 1–500 μg/mL after 24 and 72 h. Antimigratory activity was determined by wound healing method using selected concentrations of 10 and 50 μg/mL after 24 h. Untreated cells were considered as control. As control cell line, we used healthy fibroblasts (MRC‐5). Our results demonstrated abundance of LSE in phenolics, with rosmarinic acid as the main component. LSE induced low tolerance of tested planktonic probiotic strains, with no affection on their ability to form biofilm. No significant cytotoxicity on tested cancer cells was observed, with prooxidative and antimigratory effects noticed. Extract exerted significant antimigratory activity on cancer cells without effect on planktonic and probiotic cultures in biofilm. These results indicate potential application of Laetiporus sulphureus ethanolic extract as natural protector of probiotics with prominent ability to suppress cancer cell motility.

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“…Collective cell migration was analysed by wound healing (scratch) assay ( 16 ), based on making a scratch in a confluent cell layer and monitoring the behaviour and movement of cells over time. The cells at the edge of the wound began to move, filling and closing the wound.…”

Section: Methodsmentioning

confidence: 99%

Royal Jelly and Trans-10-Hydroxy-2-Decenoic Acid Inhibit Migration and Invasion of Colorectal Carcinoma Cells

Jovanović

Šeklić

Rakobradović

et al. 2022

Food Technol. Biotechnol. (Online)

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Research background. Acquisition of migratory potential is pivotal for cancer cells, enabling invasion and metastasis of colorectal carcinoma. Royal jelly and its bioactive component trans-10-hydroxy-2-decenoic acid (10H2DA) showed remarkable antimetastatic potential, but the molecular mechanism underlying this activity is unclear. Experimental approach. Identification and quantification of 10H2DA in royal jelly originating from Serbia was done by HPLC method. Cytotoxicity of 10H2DA was measured by MTT test in concentration range: 1-500 μg/mL after 24 and 72 h. Effects on collective and single cell migration was done by Wound healing and Transwell assay. Invasive potential of cancer cells was evaluated by modified Transwell method with collagen. Immunofluorescence was used for migratory and invasive protein expression, while the gene expression of these markers was evaluated by qRT-PCR. All assays were applied on HCT-116 and SW-480 cell lines and, except for MTT, evaluated after 24 h of treatment with two selected concentrations of royal jelly and 10H2DA. Results and conclusion. According to HPLC, concentration of 10H2DA in royal jelly was 0.92 % m/m. Treatment with 10H2DA showed no cytotoxic effect, however significant inhibitory potential of royal jelly and 10H2DA on motility and invasiveness of colorectal cancer cells was observed. More pronounced effect was exerted by 10H2DA that significantly suppressed collective cell migration and invasiveness of SW-480 cells, as well as single and collective cell migration and invasive potential of HCT-116 cell line. Treatments increased epithelial markers E-cadherin and cytoplasmic β-catenin in HCT-116 cells, thus stabilizing intercellular connections. In SW-480 cells, 10H2DA increased E-cadherin on protein and gene level, and suppressed EMT markers. In both cell lines treatments induced significant suppression of promigratory/proinvasive markers: N-cadherin, Vimentin and Snail on protein and gene level, which explains decreased migratory and invasive potential of HCT-116 and SW-480 cells. Novelty and scientific contribution. Our study presents new findings and elucidation of royal jelly and 10H2DA molecular mechanism that underlies their antimigratory/antiinvasive activity on colorectal cancer cells. These findings are shown hereafter for the first time indicating that these natural products are valuable source of anticancer potential and should be reconsidered for further antitumor therapy.

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Hygrophorus eburneus, edible mushroom, a promising natural bioactive agent

Cited by 6 publications

References 25 publications

Platismatia glauca —Lichen species with suppressive properties on migration and invasiveness of two different colorectal carcinoma cell lines

Platismatia glauca —Lichen species with suppressive properties on migration and invasiveness of two different colorectal carcinoma cell lines

Anticancer assessment and antibiofilm potential of Laetiporus sulphureus mushroom originated from Serbia

Royal Jelly and Trans-10-Hydroxy-2-Decenoic Acid Inhibit Migration and Invasion of Colorectal Carcinoma Cells

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