Hydroxyl Radical (ºOH) Scavenger Power of Tris (hydroxymethyl) Compared to Phosphate Buffer

Khosravifarsani, Meysam; Monfared, Ali Shabestani; Pouramir, Mahdi; Zabihi, Ebrahim

doi:10.5539/jmbr.v6n1p52

Cited by 11 publications

(4 citation statements)

References 37 publications

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“…[ 19 ] Additionally, phosphate buffer is found to act as a radical scavenger, which might explain (together with the aforementioned increased deformation resistance) the lower degree of hydrogel contraction we have observed in our γ ‐irradiation experiments (Figure 2B,C). [ 20 ] The reactivity of the residual alkyne groups suggested that it should be possible to glue [ 21 ] or fuse hydrogel objects using γ ‐irradiation ( Figure A). We designed an experiment in which two hydrogel cubes where placed on top of each other inside a closed glass vial.…”

Section: Resultsmentioning

confidence: 99%

Gamma Radiation Induced Contraction of Alkyne Modified Polymer Hydrogels

Brevé

Liu

Denkova

et al. 2021

Macro Materials & Eng

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Gamma radiation triggered secondary crosslinking of dextran hydrogels leads to macroscopic hydrogel contraction. The authors use stable polymer hydrogels, prepared through azide-alkyne crosslinking, containing surplus alkyne groups. 𝜸-irradiation of these gels leads to more alkyne crosslinking, enabling controlled increase of crosslink density, which in turn leads to an increase of hydrogel stiffness and macroscopic hydrogel contraction. Gel contraction scales linearly with the applied radiation dose. The same mechanism is applied to achieve 𝜸-radiation triggered release of the small molecule cargo, akin to wringing out a sponge. 𝜸-irradiation of touching hydrogel objects leads to gel fusion and the formation of a self-supporting gel connection, demonstrating the reactivity of the excess alkyne groups. They envision applications in gel gluing and the construction of complex gel architectures, as well as in responsive materials for controlled release.

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Section: Resultsmentioning

confidence: 99%

Gamma Radiation Induced Contraction of Alkyne Modified Polymer Hydrogels

Brevé

Liu

Denkova

et al. 2021

Macro Materials & Eng

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“…Previous studies on the effect of plasma on DNA in aqueous solutions partially helped us to select an appropriate culture medium for the collected semen samples. It has been suggested that complex media could interact with the plasma components (particularly ROS) [ 22 ] or change their properties (such as pH) in response to NTP treatment [ 23 ]. Similarly, data collected from the chemiluminescent assay for ROS quantification should be interpreted with caution due to a variety of factors that may affect the signal obtained, such as the medium composition or its pH [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Non-Thermal Plasma on the Structural and Functional Characteristics of Human Spermatozoa

Tvrdá

Lovíšek

Kyzek

et al. 2021

IJMS

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Significant antibacterial properties of non-thermal plasma (NTP) have converted this technology into a promising alternative to the widespread use of antibiotics in assisted reproduction. As substantial data available on the specific in vitro effects of NTP on male reproductive cells are currently missing, this study was designed to investigate selected quality parameters of human spermatozoa (n = 51) exposed to diffuse coplanar surface barrier discharge NTP for 0 s, 15 s, 30 s, 60 s and 90 s. Sperm motility characteristics, membrane integrity, mitochondrial activity, production of reactive oxygen species (ROS), DNA fragmentation and lipid peroxidation (LPO) were investigated immediately following exposure to NTP and 2 h post-NTP treatment. Exposure to NTP with a power input of 40 W for 15 s or 30 s was found to have no negative effects on the sperm structure or function. However, a prolonged NTP treatment impaired all the sperm quality markers in a time- and dose-dependent manner. The most likely mechanism of action of high NTP doses may be connected to ROS overproduction, leading to plasma membrane destabilization, LPO, mitochondrial failure and a subsequent loss of motility as well as DNA integrity. As such, our findings indicate that appropriate plasma exposure conditions need to be carefully selected in order to preserve the sperm vitality, should NTP be used in the practical management of bacteriospermia in the future.

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“…25 Additionally, phosphate buffer is found to act as a radical scavenger, which might explain the lower degree of hydrogel contraction we have observed in our g-irradiation experiments (Figure 2B and 2C). 26 The reactivity of the residual alkyne groups suggested that it should be possible to glue 27,28 or fuse hydrogel objects using g-irradiation (Figure 3A). We designed an experiment in which two hydrogel cubes where placed on top of each other inside a closed glass vial.…”

mentioning

confidence: 99%