Hydroxyethyl methacrylate grafted carboxy methyl tamarind (CMT-g-HEMA) polysaccharide based matrix as a suitable scaffold for skin tissue engineering

Choudhury, Priyanka; Kumar, Satish; Singh, Asmita; Kumar, Ashutosh; Kaur, Navneet; Sanyasi, Sridhar; Chawla, Saurabh; Goswami, Chandan; Goswami, Luna

doi:10.1016/j.carbpol.2018.01.079

Cited by 21 publications

(12 citation statements)

References 48 publications

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“…We have previously described the synthesis and characterization of a polysaccharide based hydrogel CMT:HEMA as an effective biocompatible surface which can act as a suitable material for bone tissue engineering 18 . Recently we have also shown that this hydrogel is biocompatible for skin cells as well as murine derived Mesenchymal stem cells 19 . This hydrogel doesnot have any adverse effects when applied on the skin of live rats, indicating its suitability for in vivo applications 19 .…”

Section: Trpm8 Inhibition Induces Differentiation Of Rat Calvarial Osmentioning

confidence: 84%

“…Recently we have also shown that this hydrogel is biocompatible for skin cells as well as murine derived Mesenchymal stem cells 19 . This hydrogel doesnot have any adverse effects when applied on the skin of live rats, indicating its suitability for in vivo applications 19 . Therefore, we have characterized this hydrogel as a suitable surface and medium for sustained release of drugs.…”

Section: Trpm8 Inhibition Induces Differentiation Of Rat Calvarial Osmentioning

confidence: 84%

“…We have previously shown CMT:HEMA as a suitable biomaterial for successful adhesion and growth of osteoclasts and keratinocytes 18,19 . We now demonstrate that the same hydrogel is suitable for osteoblasts and Mesenchymal Stem Cells.…”

Section: Discussionmentioning

confidence: 99%

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TRPM8 channel inhibitor-encapsulated hydrogel as a tunable surface for bone tissue engineering

Acharya

Kumar

Tiwari

et al. 2021

Sci Rep

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A major limitation in the bio-medical sector is the availability of materials suitable for bone tissue engineering using stem cells and methodology converting the stochastic biological events towards definitive as well as efficient bio-mineralization. We show that osteoblasts and Bone Marrow-derived Mesenchymal Stem Cell Pools (BM-MSCP) express TRPM8, a Ca2+-ion channel critical for bone-mineralization. TRPM8 inhibition triggers up-regulation of key osteogenesis factors; and increases mineralization by osteoblasts. We utilized CMT:HEMA, a carbohydrate polymer-based hydrogel that has nanofiber-like structure suitable for optimum delivery of TRPM8-specific activators or inhibitors. This hydrogel is ideal for proper adhesion, growth, and differentiation of osteoblast cell lines, primary osteoblasts, and BM-MSCP. CMT:HEMA coated with AMTB (TRPM8 inhibitor) induces differentiation of BM-MSCP into osteoblasts and subsequent mineralization in a dose-dependent manner. Prolonged and optimum inhibition of TRPM8 by AMTB released from the gels results in upregulation of osteogenic markers. We propose that AMTB-coated CMT:HEMA can be used as a tunable surface for bone tissue engineering. These findings may have broad implications in different bio-medical sectors.

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Section: Trpm8 Inhibition Induces Differentiation Of Rat Calvarial Osmentioning

confidence: 84%

Section: Trpm8 Inhibition Induces Differentiation Of Rat Calvarial Osmentioning

confidence: 84%

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TRPM8 channel inhibitor-encapsulated hydrogel as a tunable surface for bone tissue engineering

Acharya

Kumar

Tiwari

et al. 2021

Sci Rep

Self Cite

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“…Hydroxyethyl methacrylate (HEMA) is a water-soluble 8,9 monomer and is widely accepted as a material used in the eld of drug delivery. [10][11][12][13][14] HEMA has been used as a scaffold in skin tissue engineering [15][16][17][18][19][20] and as a hybrid hydrogel of xylan, HEMA and mesoporous silica has been studied as a scaffold for the growth and attachment of broblasts. 21 In the present study, HEMA was used in the scaffold system to overcome the limitation of mechanical instability.…”

Section: Introductionmentioning

confidence: 99%

Montmorillonite stabilized chitosan-co-mucin hydrogel for tissue engineering applications

2021

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“…Further, the introduction of carboxymethyl groups in TKP make the polymer anionic and therefore, it will definitely enhance the reactivity of TKP towards graft copolymerization to a greater extent. The literature survey reveals that graft copolymerization of acrylamide, [ 13 ] acrylonitrile [ 14 ] and hydroxy ethyl methacrylate [ 15 ] has been carried out onto Na‐PCMTKP using only the chemical method. However, there are no reports with regard to the modification of Na‐PCMTKP through low‐energy‐initiated graft copolymerization using CAN as a photo‐initiator.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of Photo‐Graft Copolymers of Sodium Salt of Partially Carboxymethylated Tamarind Kernel Powder

Trivedi

Joshi

Trivedi

2021

Macromolecular Symposia

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In the present case, the functionalization of sodium salt of partially carboxymethylated tamarind kernel powder (Na-PCMTKP, DS= 0.15), an industrially important natural polymer, is carried out through photo-graft copolymerization of methyl methacrylate (MMA) and ethyl methacrylate (EMA) onto it using ceric ammonium nitrate (CAN) as a photo-initiator. The effects of the concentrations of photo-initiator (CAN), nitric acid, methacrylate monomers (MMA and EMA) and the amount of Na-PCMTKP as well as reaction time and temperature on photo-graft copolymerization are being investigated to achieve the maximum graft yields (%G = 232.2 and %GE = 91.0 in the case of MMA and %G = 206.2 and %GE = 90.0 in the case of EMA). A suitable mechanism to explain the photo-graft copolymerization is being proposed. The kinetics aspect of the photo-graft copolymerization is also studied and the values of the overall activation energy for the photo-graft copolymerization in the case of MMA and EMA are found to be 8.10 and 10.49 kJ mol −1 , respectively. Besides kinetics study, the chemical structure and thermal properties of the graft copolymers of Na-PCMTKP containing PMMA and PEMA is studied by FTIR, TGA, and SEM techniques and the results ascertains the evidence of the successful photo-graft copolymerization of both the methacrylate monomers onto Na-PCMTKP. The synthesized novel graft copolymers may find their potential applications to be used as metal adsorbents as well as novel excipients for direct compression matrix tablets.

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Hydroxyethyl methacrylate grafted carboxy methyl tamarind (CMT-g-HEMA) polysaccharide based matrix as a suitable scaffold for skin tissue engineering

Cited by 21 publications

References 48 publications

TRPM8 channel inhibitor-encapsulated hydrogel as a tunable surface for bone tissue engineering

TRPM8 channel inhibitor-encapsulated hydrogel as a tunable surface for bone tissue engineering

Montmorillonite stabilized chitosan-co-mucin hydrogel for tissue engineering applications

Synthesis and Characterization of Photo‐Graft Copolymers of Sodium Salt of Partially Carboxymethylated Tamarind Kernel Powder

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