Hydroxyapatite surface roughness: Complex modulation of the osteoclastogenesis of human precursor cells

Costa‐Rodrigues, João; Fernandes, Anabela C.; Lopes, M. A.; Fernandes, Maria Helena

doi:10.1016/j.actbio.2011.11.032

Cited by 71 publications

(63 citation statements)

References 37 publications

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“…As for OC differentiation, literature has mainly focused on surface roughness but this is a parameter that would change according to surface micropore and grain size. Costa-Rodrigues et al (2012) (text reference) reported that on HA, osteoclast differentiation of human precursors was promoted by surface roughness similar to that of osteoinductive TCPs here.…”

Section: Brennansupporting

confidence: 65%

Submicron-scale surface architecture of tricalcium phosphate directs osteogenesis in vitro and in vivo

Davison

Luo²,

Schoenmaker³

et al. 2014

eCM

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A current challenge of synthetic bone graft substitute design is to induce bone formation at a similar rate to its biological resorption, matching bone's intrinsic osteoinductivity and capacity for remodelling. We hypothesise that both osteoinduction and resorption can be achieved by altering surface microstructure of beta-tricalcium phosphate (TCP). To test this, two TCP ceramics are engineered with equivalent chemistry and macrostructure but with either submicron-or micron-scale surface architecture. In vitro, submicron-scale surface architecture differentiates larger, more active osteoclasts -a cell type shown to be important for both TCP resorption and osteogenesis -and enhances their secretion of osteogenic factors to induce osteoblast differentiation of human mesenchymal stem cells. In an intramuscular model, submicrostructured TCP forms 20 % bone in the free space, is resorbed by 24 %, and is densely populated by multinucleated osteoclast-like cells after 12 weeks; however, TCP with micron-scale surface architecture forms no bone, is essentially not resorbed, and contains scarce osteoclast-like cells. Thus, a novel submicron-structured TCP induces substantial bone formation and is resorbed at an equivalent rate, potentially through the control of osteoclast-like cells.

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Section: Brennansupporting

confidence: 65%

Submicron-scale surface architecture of tricalcium phosphate directs osteogenesis in vitro and in vivo

Davison

Luo²,

Schoenmaker³

et al. 2014

eCM

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show abstract

“…Therefore, it is likely that also osteoclast differentiation and activity is modulated by these parameters, even if osteoclasts show a certain degree of 'rugophilia' [28].…”

Section: Discussionmentioning

confidence: 99%

Osteoclast differentiation from human blood precursors on biomimetic calcium-phosphate substrates

et al. 2017

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“…These bioceramics will also be able to activate the cellular response to recruit osteoclasts, osteoblasts and endothelial cells to the implant site for the resorbtion of the implant whilst enhancing the formation of new living bone [2,7,8,[18][19][20]. Many different factors influence the activity of bone in the body which makes it crucial to understand the dynamics of bone and its interaction with the environment before real advances can be made in bioceramics for bone replacement and repair, as well as tissue engineering [4,21,22].…”

Section: Introductionmentioning

confidence: 99%

In vitro osteoclast-like and osteoblast cells’ response to electrospun calcium phosphate biphasic candidate scaffolds for bone tissue engineering

Wepener

Richter

Papendorp

et al. 2012

J Mater Sci: Mater Med

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Successful long term bone replacement and repair remain a challenge today. Nanotechnology has makes it possible to alter materials' characteristics and therefore possibly improve on the material itself. In this study, biphasic (hydroxyapatite/β-tricalcium phosphate (HA/β-TCP)) nanobioceramic scaffolds were prepared by the electrospinning technique in order to mimic the extracellular matrix (ECM). Scaffolds were characterised by scanning electron microscopy (SEM) and Attentuated Total Reflectance Fourier Transform Infrared (ATR-FTIR). Osteoblasts as well as monocytes that were differentiated into osteoclast-like cells, were cultured separately on the biphasic bioceramic scaffolds for up to 6 days and the proliferation, adhesion and cellular response were determined using lactate dehydrogenase (LDH) cytotoxicity assay, nucleus and cytoskeleton dynamics, analysis of the cell cycle progression, measurement of the mitochondrial membrane potential and the detection of phosphatidylserine expression. SEM analysis of the biphasic bioceramic scaffolds revealed n ano fi be rs spun i n a m e sh -like sc af fold . Re sul ts indi c ate th at th e bi ph asi c bio ce ram ic electrospun scaffolds are biocompatible and have no significant negative effects on either osteoblasts or osteoclast-like cells in vitro.

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Hydroxyapatite surface roughness: Complex modulation of the osteoclastogenesis of human precursor cells

Cited by 71 publications

References 37 publications

Submicron-scale surface architecture of tricalcium phosphate directs osteogenesis in vitro and in vivo

Submicron-scale surface architecture of tricalcium phosphate directs osteogenesis in vitro and in vivo

Osteoclast differentiation from human blood precursors on biomimetic calcium-phosphate substrates

In vitro osteoclast-like and osteoblast cells’ response to electrospun calcium phosphate biphasic candidate scaffolds for bone tissue engineering

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