Hydroxyapatite scaffolds hydrothermally grown from aragonitic cuttlefish bones

Rocha, J.H.G.; Lemos, A.F.; Kannan, S.; Agathopoulos, Simeon; Ferreira, J.M.F.

doi:10.1039/b510122k

Cited by 88 publications

(60 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It was obtained a AB-type carbonated HA, which is similar to the composition of human bones [122,193]. Additionally, the size of HA crystallites was similar to the size of bone-like apatite (20-50 nm) [122]. The obtained scaffolds demonstrated to have a high thermal stability on sintering up to 1350°C.…”

Section: Cuttlefish Bonementioning

confidence: 63%

“…The CB internal structure was not compromised during the HT and thereby the pore size and interconnectivity was maintained after the transformation [122,188,193]. It was obtained a AB-type carbonated HA, which is similar to the composition of human bones [122,193]. Additionally, the size of HA crystallites was similar to the size of bone-like apatite (20-50 nm) [122].…”

Section: Cuttlefish Bonementioning

confidence: 75%

“…The mixture was sealed in polytetrafluoroethylene (Teflon) lined stainless steel autoclave and the hydrothermal treatment (HT) was conducted at 200°C. The CB internal structure was not compromised during the HT and thereby the pore size and interconnectivity was maintained after the transformation [122,188,193]. It was obtained a AB-type carbonated HA, which is similar to the composition of human bones [122,193].…”

Section: Cuttlefish Bonementioning

confidence: 80%

“…The conversion from corals to porous HA was first performed by Roy and Linnehan in 1974 [118]. Since then, marine skeletons of cuttlefish [122], marine sponges skeletons [123] and nacre seashell [124] have been converted to HA, while maintaining their original structures, aiming at obtaining bone graft substitutes. In turn, sea urchin spines that consist of large crystals of Mg-rich calcite [(Ca,Mg)CO3] have been hydrothermally transfored into Mg-substituted TCP [125] has been used as templates with optimal range of pore size, channels, and structural network for bone growth.…”

Section: Marine-derived Bioceramic As Scaffolds For Bone Tissue Enginmentioning

confidence: 99%

“…The transformation of CB into HA through a hydrothermal process was firstly reported by Rocha et al [122,188,193]. Prior to the transformation, the exact aragonite content in CB was analysed and the corresponding amount of (NH4)2H2PO4 was added to set the molar ratio of Ca/P = 1.67.…”

Section: Cuttlefish Bonementioning

confidence: 99%

See 4 more Smart Citations

Synthetic and Marine-Derived Porous Bone Graft Substitutes

Neto¹,

Ferreira²

2018

Preprint

View full text Add to dashboard Cite

Bone is a dynamic tissue with the capacity of repair and regeneration in specific conditions. Nevertheless, due to the increased incidence of bone disorders, the need of bone grafts has been growing over the past decades and the development of a bone graft with optimal properties remains a clinical challenge. This review addresses the bone properties (morphology, composition and their repair and regeneration capacity) and then is focused on the potential strategies for bone repair and regeneration. It describes the requirements for designing a suitable scaffold material, types of materials (polymers, ceramics and composites) and techniques to obtain the porous structures (additive manufacturing techniques/robocasting or derived from marine skeletons) for bone tissue engineering applications. The main objective of this review is to gather the knowledge on the materials and methods for the production of scaffolds for bone tissue engineering and highlight that natural materials, namely the marine skeletons represent a promising alternative.

show abstract

Section: Cuttlefish Bonementioning

confidence: 63%

Section: Cuttlefish Bonementioning

confidence: 75%

Section: Cuttlefish Bonementioning

confidence: 80%

Section: Marine-derived Bioceramic As Scaffolds For Bone Tissue Enginmentioning

confidence: 99%

Section: Cuttlefish Bonementioning

confidence: 99%

See 3 more Smart Citations

Synthetic and Marine-Derived Porous Bone Graft Substitutes

Neto¹,

Ferreira²

2018

Preprint

View full text Add to dashboard Cite

show abstract

Thermomechanical Analysis of a Bio‐Inspired Lightweight Multifunctional Structure

et al. 2020

View full text Add to dashboard Cite

Cuttlebone constitutes the inner shell of a cuttlefish, and it has a highly porous chambered microstructure of about 93% porosity by volume. [1] Cuttlebone functions as a skeletal element and a rigid buoyancy tank, which allows the organism to control its overall density by regulating the gas/liquid ratio inside its porous structure. [2] As some cuttlefish can live up to 600 m deep in the ocean, the partially gas-filled cuttlebone is required to withstand extreme compressive loading from the water pressure (up to 60 atm). [3,4] Previous studies have suggested that cuttlebone's remarkable damage tolerance and high stiffness can be attributed to its characteristic "wall-septa" internal morphology. [5] Several previous studies on cuttlebone have explored its potential applications by exploiting its chemical, structural, and mechanical characteristics as a natural cellular material. For example, cuttlebone itself can be used as a filler in natural rubber [6] and acrylic bone cement, [7] the mechanical properties of which are comparable to commercial products. In addition, a poly coated hydroxyapatite scaffold derived from cuttlebone through a hydrothermal transformation process was shown to exhibit enhanced mechanical properties and is a good candidate for bone tissue engineering applications. [8-10] Previous research on bio-inspired structures based on cuttlebone has generally focused on structural applications, motivated by cuttlebone's low density and high strength. [1,4,11,12] Currently, little research has been carried out to explore the potential multifunctional performance of cuttleboneinspired structures. In particular, the "wall-septa" morphology of cuttlebone resembles the arrangement of a plate and pins in a typical pin-fin heat sink [13,14] as well as the sandwich structure used in an integrated thermal protection system (ITPS) for spacecraft vehicles. [15-17] The combination of high mechanical performance (stiffness and damage tolerance), low density, and pin-fin-like configuration motivates us to explore the potential thermomechanical applications of cuttlebone-inspired structures. In addition to computationally analyzing the mechanical performance of the cuttlebone structure based on high-resolution 3D structural reconstruction, this research systematically analyzes the feasibility of using cuttlebone as an inspiration for the design of novel lightweight multifunctional structures for thermal management applications, including an air-cooled heat sink for electronic devices and an ITPS for spacecraft vehicles. Regarding the air-cooled heat sink evaluation, devising effective thermal management methods for electronic components are becoming increasingly difficult due to the demand for higher performance and the continual miniaturization of electronic devices. Numerous studies have been conducted to analyze heat sinks with natural convection, including the effect of pin fin orientation and pressure drop on local heat transfer, [18-22] effective fin shape and height for maximum heat transfer, [23-25] and e...

show abstract

Nonenzymatic Transformation of Amorphous CaCO₃ into Calcium Phosphate Mineral after Exposure to Sodium Phosphate in Vitro: Implications for in Vivo Hydroxyapatite Bone Formation

et al. 2015

View full text Add to dashboard Cite

Studies indicate that mammalian bone formation is initiated at calcium carbonate bioseeds, a process that is driven enzymatically by carbonic anhydrase (CA). We show that amorphous calcium carbonate (ACC) and bicarbonate (HCO3 (-) ) cause induction of expression of the CA in human osteogenic SaOS-2 cells. The mineral deposits formed on the surface of the cells are rich in C, Ca and P. FTIR analysis revealed that ACC, vaterite, and aragonite, after exposure to phosphate, undergo transformation into calcium phosphate. This exchange was not seen for calcite. The changes to ACC, vaterite, and aragonite depended on the concentration of phosphate. The rate of incorporation of phosphate into ACC, vaterite, and aragonite, is significantly accelerated in the presence of a peptide rich in aspartic acid and glutamic acid. We propose that the initial CaCO3 bioseed formation is driven by CA, and that the subsequent conversion to calcium phosphate/calcium hydroxyapatite (exchange of carbonate by phosphate) is a non-enzymatic exchange process.

show abstract

Hydroxyapatite scaffolds hydrothermally grown from aragonitic cuttlefish bones

Cited by 88 publications

References 21 publications

Synthetic and Marine-Derived Porous Bone Graft Substitutes

Synthetic and Marine-Derived Porous Bone Graft Substitutes

Thermomechanical Analysis of a Bio‐Inspired Lightweight Multifunctional Structure

Nonenzymatic Transformation of Amorphous CaCO₃ into Calcium Phosphate Mineral after Exposure to Sodium Phosphate in Vitro: Implications for in Vivo Hydroxyapatite Bone Formation

Contact Info

Product

Resources

About

Hydroxyapatite scaffolds hydrothermally grown from aragonitic cuttlefish bones

Cited by 88 publications

References 21 publications

Synthetic and Marine-Derived Porous Bone Graft Substitutes

Synthetic and Marine-Derived Porous Bone Graft Substitutes

Thermomechanical Analysis of a Bio‐Inspired Lightweight Multifunctional Structure

Nonenzymatic Transformation of Amorphous CaCO3 into Calcium Phosphate Mineral after Exposure to Sodium Phosphate in Vitro: Implications for in Vivo Hydroxyapatite Bone Formation

Contact Info

Product

Resources

About

Nonenzymatic Transformation of Amorphous CaCO₃ into Calcium Phosphate Mineral after Exposure to Sodium Phosphate in Vitro: Implications for in Vivo Hydroxyapatite Bone Formation