Hydrophobic fractions from Strongyloides venezuelensis for use in the human immunodiagnosis of strongyloidiasis

Feliciano, Nágilla Daliane; Gonzaga, Henrique Tomaz; Gonçalves-Pires, Maria do Rosário F.; Gonçalves, Ana Lúcia Ribeiro; Rodrigues, Raphael Silva; Ueta, Marlene Tiduko; Costa-Cruz, Júlia Maria

doi:10.1016/j.diagmicrobio.2010.01.012

Cited by 48 publications

(57 citation statements)

References 34 publications

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“…The difficulty in recovering sufficient quantities of S. stercoralis larvae for use in antigenic preparations and results achieved using S. venezuelensis antigens reinforce the fact that this parasite shares important antigenic determinants with S. stercoralis and in sufficient concentrations for use in the human strongyloidiasis immunodiagnosis, supporting applicability of heterologous antigen as a reliable diagnostic tool [5][6][7].…”

Section: Discussionmentioning

confidence: 75%

“…Major advantages of using fractioned antigen with TX-114 are: reduced cost of production in laboratory, simple and easy extraction and high efficiency [31]. Considering usefulness of S. venezuelensis anti- gen and the fact that fractions obtained from TX-114, mainly D phase, are highly sensitive and specific [7,27,32] the potential of this fraction to detect IgA and IgG anti-Strongyloides in different biological samples: serum and saliva was analyzed.…”

Section: Discussionmentioning

confidence: 99%

“…Total saline extract of S. venezuelensis (SE) was submitted to protein separation using Triton X-114, according to Feliciano et al [7]. Briefly, Tris-buffered saline (TBS), pH 7.4 (10 mmol/L Tris-HCl, 150 mmol/L NaCl), was added to the SE followed by 1% TX-114 and the solution was incubated at 0 • C for 10 min.…”

Section: Obtention Of the Detergent Phase (D)mentioning

confidence: 99%

“…As strongyloidiasis immunodiagnosis continues to be a challenge because of the increasing difficulty in obtaining sufficient quantities of S. stercoralis larvae [4,5], alternative antigens, including heterologous antigens from Strongyloides venezuelensis have also been used with satisfactory results to diagnose human strongyloidiasis [6,7].…”

Section: Introductionmentioning

confidence: 99%

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Detergent fraction of heterologous antigen to detect IgA and IgG in strongyloidiasis using saliva and serum paired samples

et al. 2010

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Strongyloides stercoralis causes chronic asymptomatic infections in immunocompetent human hosts and systemic invasion in immunocompromised patients, developing into a fatal hyperinfection syndrome. IgA and IgG detection in saliva and serum paired samples were tested using total saline extract from Strongyloides venezuelensis (SE(*)) and its detergent phase (D) extracted with Triton X-114. Saliva and serum paired samples were obtained from: 25 patients with confirmed strongyloidiasis; 25 patients with other parasitoses and 20 from apparently healthy individuals. Sensitivity, specificity, diagnostic efficiency, positive and negative predictive values and likelihood ratio were calculated at the optimum point of reaction. Using D phase sensitivity and specificity to detect IgA in saliva were 76.0% and 88.9% and in serum 80.0% and 86.7%, respectively. To detect IgG, D phase showed sensitivity and specificity of 88.0% and 88.9% in saliva and 88.0% and 84.4% in serum, respectively. D phase proved to be specific and efficient and could be utilized as an alternative antigen for IgA and IgG detection in saliva and serum samples for strongyloidiasis diagnosis.

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Section: Discussionmentioning

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Section: Obtention Of the Detergent Phase (D)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Detergent fraction of heterologous antigen to detect IgA and IgG in strongyloidiasis using saliva and serum paired samples

et al. 2010

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“…The feasibility of this congeneric species approach has already been demonstrated by the diagnoses of patients who are infected with parasites that share epitopes, such as S. stercoralis and Strongyloides venezuelensis (Feliciano et al 2010) or Strongyloides ratti (Rodrigues et al 2007). This approach has been particularly promising in the development of alternative methods for the diagnoses of cisticercosis, strongyloidiasis and hidatidosis (Vaz et al 1997, Sako et al 2006, Feliciano et al 2010, Oliveira et al 2010, da S Ribeiro et al 2010). In addition, Dekumyoy et al (2000) studied the use of a crude antigen preparation that was derived from A. costaricensis as a heterologous target for the detection of antibodies in patients with eosinophilic meningitis.…”

Section: Discussionmentioning

confidence: 99%

Use of heterologous antigens for the immunodiagnosis of abdominal angiostrongyliasis by an enzyme-linked immunosorbent assay

Ben

Rodrigues

Agostini

et al. 2010

Mem. Inst. Oswaldo Cruz

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Angiostrongylus costaricensis has a broad geographic distribution spanning from North to South America and the infections of vertebrates with this nematode can result in abdominal complications. Human infections are diagnosed by histological or serological methods because the isolation of larvae from feces is not feasible, as most parasites become trapped in intestinal tissues due to intense eosinophilic inflammation. Because A. costaricensis is difficult to maintain in the laboratory, an immunodiagnostic IgG enzyme-linked immunosorbent assay (ELISA) using antigens from the congeneric Angiostrongylus cantonensis species was evaluated against a panel of serum samples from patients who were histologically diagnosed with A. costaricensis infections. Sera from uninfected individuals and individuals infected with other parasites were used as controls. The sensitivity and specificity of the assay were estimated at 88.4% and 78.7%, respectively. Because the use of purified or cloned antigens has not been established as a reliable diagnostic tool, the use of heterologous antigens may provide a viable alternative for the development of an ELISA-based immunodetection system for the diagnosis of abdominal angiostrongyliasis

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Diethylaminoethyl (DEAE) binding fraction from Taenia solium metacestode improves the neurocysticercosis serodiagnosis

et al. 2014

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Neurocysticercosis (NC) is one of the most important diseases caused by parasites affecting the central nervous system. We fractionated by ion-exchange chromatography using diethylaminoethyl (DEAE)-sepharose resin the total saline extract (S) from Taenia solium metacestodes and evaluated obtained fractions (DEAE S1 and DEAE S2) by enzyme-linked immunosorbent assay (ELISA, n = 123) and immunoblotting (IB, n = 22) to detect human NC in serum. Diagnostic parameters were established by ROC and TG ROC curves for ELISA tests. IB was qualitatively analyzed. S and DEAE S1 presented sensitivity of 87. 5% and DEAE S2 90%. The best specificity was observed for DEAE S2 (90.4%). In IB, using DEAE S2 samples from NC patients presented bands of 20-25, 43-45, 55-50, 60-66, 82, 89, and 140 kDa. The great diagnostic parameters reached by DEAE S2 suggest the potential applicability of this fraction in NC immunodiagnosis.

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Hydrophobic fractions from Strongyloides venezuelensis for use in the human immunodiagnosis of strongyloidiasis

Cited by 48 publications

References 34 publications

Detergent fraction of heterologous antigen to detect IgA and IgG in strongyloidiasis using saliva and serum paired samples

Detergent fraction of heterologous antigen to detect IgA and IgG in strongyloidiasis using saliva and serum paired samples

Use of heterologous antigens for the immunodiagnosis of abdominal angiostrongyliasis by an enzyme-linked immunosorbent assay

Diethylaminoethyl (DEAE) binding fraction from Taenia solium metacestode improves the neurocysticercosis serodiagnosis

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