Hydrophilic modification of polystyrene with hydrophobin for time-resolved immunofluorometric assay

Wang, Zefang; Huang, Yujian; Li, Shan; Xu, Haijin; Linder, Markus B.; Qiao, Mingqiang

doi:10.1016/j.bios.2010.08.059

Cited by 45 publications

(33 citation statements)

References 27 publications

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“…The oldest processes are wet chemical techniques. [16][17][18][19][20] Polystyrene functionalization employing atomic oxygen treatments 17,21 is a well-documented example of such wet chemistry approaches. However, the complex downstream treatment of costly and sometimes hazardous solvents hinders the application of wet chemistry approaches on a larger scale.…”

Section: Introductionmentioning

confidence: 99%

Gas-Phase Surface Engineering of Polystyrene Beads Used to Challenge Automated Particle Inspection Systems

Labonté

Marion

Virgilio

2016

Ind. Eng. Chem. Res.

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Container challenge sets, used in the qualification and validation of automated visible particle inspection systems in the parenteral drug industry, are prepared by seeding a single standardized polystyrene-divinylbenzene (PS-DVB) bead inside the commercial product to mimic foreign particulates. Due to its low surface energy and wettability, the bead adheres to container walls, hindering its detection by the motion based inspection system. The aim of this research is to modify the surface properties of the bead in such a way that it repulses the inner walls and stays in suspension inside the liquid product. The surface treatment consists of a photoinduced chemical vapor deposition (PICVD) process using syngas and UVC light. Following 2 treatment, newly grafted C-OH, C-O-C, C=O and COOH functional groups on the bead's surface are observed by XPS and FTIR spectroscopy, leading to an increase in the surface energy from 31 ± 1 to 65 ± 2 mJ/m 2 , and a corresponding zeta potential decrease from -38 mV to -61 mV.Finally, treated 100 µm, 200 µm and 500 µm PS-DVB beads suspended in water exhibit higher dispersion stability over time than untreated beads. These results show the potential of syngas PICVD to provide an effective solution to the stability issue of containers challenge sets for the validation of automated particle inspection systems, enabling significant savings of time and money to the parenteral drug industry.

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Section: Introductionmentioning

confidence: 99%

Gas-Phase Surface Engineering of Polystyrene Beads Used to Challenge Automated Particle Inspection Systems

Labonté

Marion

Virgilio

2016

Ind. Eng. Chem. Res.

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“…Most importantly, a discrete portion of their exposed surface is composed of amino acids with hydrophobic sidechains; this hydrophobic patch endows hydrophobins with exceptional amphiphilic properties and drives their spontaneous and rapid self-assembly at hydrophobic/hydrophilic interfaces, such as air/water and oil/water boundaries, where they pack into ordered structures and form remarkably strong and elastic fi lms. [ 22,23 ] Hydrophobins also effi ciently assemble onto solid surfaces to form amphiphilic fi lms that are able to reverse the surface wettability of the coated material [ 24 ] and allow for the immobilization of, e.g., proteins or enzymes onto solid surfaces [25][26][27][28] while preserving all of the features of the immobilized biomolecules. [ 29 ] The main advantage of this method is its simplicity: hydrophobin self-assembly proceeds spontaneously at room temperature within seconds or minutes and requires only a very tiny amount of protein.…”

Section: Introductionmentioning

confidence: 99%

Hydrophobin as a Nanolayer Primer That Enables the Fluorinated Coating of Poorly Reactive Polymer Surfaces

Gazzera

Corti

Pirrie

et al. 2015

Adv Materials Inter

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thick fi lm to achieve signifi cant durability and, therefore, requires a relatively large amount of fl uoropolymer. Alternative approaches, such as chemical grafting of fl uoropolymers by radical methods through irradiation, [ 6−8 ] plasma, [ 9−12 ] or direct fl uorination with fl uorine gas, [ 1,2,13 ] require only moderate amounts of fl uorinated surface modifi ers but are much more aggressive and, in some cases, potentially hazardous.Other possibilities involve functionalization with fl uorinated molecules/ polymers consisting of specifi c chemical moieties that are able to bind either covalently or noncovalently to the polymer surface. [ 4,5,14,15 ] However, to achieve sufficiently stable bonding, this general strategy requires the presence of reactive hydrophilic sites, typically OH groups, which are not typically observed on the surfaces of apolar, hydrophobic polymers, such as polyolefi ns, and need to be introduced via oxidizing pretreatments that are usually either energy-intensive or not environmentally friendly. [ 16−19 ] Expanding on this strategy, we present an effi cient, rapid, and more environmentally friendly method to perform the fl uorocarbon coating of hydrophobic polymer surfaces. The method is based on the use of hydrophobins, i.e., amphiphilic surface-active proteins, as a nanosized primer layer that adheres to the hydrophobic polymer surface, making the surface hydrophilic and preparing it for the subsequent binding of a fl uoropolymer containing ionic moieties.Hydrophobins are a class of nontoxic, surface-active, and fi lm-forming proteins that are produced by fi lamentous A new and simple method is presented to fl uorinate the surfaces of poorly reactive hydrophobic polymers in a more environmentally friendly way using the protein hydrophobin (HFBII) as a nanosized primer layer. In particular, HFBII, via electrostatic interactions, enables the otherwise ineffi cient binding of a phosphate-terminated perfl uoropolyether onto polystyrene, polypropylene, and low-density polyethylene surfaces. The binding between HFBII and the perfl uoropolyether depends signifi cantly on the environmental pH, reaching the maximum stability at pH 4. Upon treatment, the polymeric surfaces mostly retain their hydrophobic character but also acquire remarkable oil repellency, which is not observed in the absence of the protein primer. The functionalization proceeds rapidly and spontaneously at room temperature in aqueous solutions without requiring energy-intensive procedures, such as plasma or irradiation treatments.

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“…In contrast, biomolecules have less conformational change and retain functional activity when are immobilized on hydrophilic surfaces, as the major driving force between biomolecules and the hydrophilic surface is the electrostatic force (Goddard and Hotchkiss, 2007;Kaur et al, 2004;Lubarsky et al, 2005). Moreover the strength and selectivity of protein-protein interactions make proteins excellent candidates to serve as linkers to form ordered structures (Wang 2010). Taking into consideration the above mentioned evidences, self assembling proteins like hydrophobins that have the remarkable property of adhering to almost any surface forming stable amphiphylic films are very good candidates to easily manufacture stable, enzymebased catalytic surfaces for applications in biosensing.…”

Section: Biosensors Based On Organic/inorganic Interfacesmentioning

confidence: 99%

“…In particular, time resolved fluorescence assay has been used for the quantitative determination of carcinoembryonic antigen. A detection limit of 0.24 ng/mL is claimed by authors (Wang et al 2010). Even if class II is less chemically stable, HFBI has been used in glucose biosensing once decorated by multi wall carbon nanotube and glucose oxidase (Wang et al 2010).…”

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confidence: 99%

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Organic-inorganic Interfaces for a New Generation of Hybrid Biosensors

Rea¹,

Giardina²,

Longobardi³

et al. 2011

Biosensors - Emerging Materials and Applications

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Hydrophilic modification of polystyrene with hydrophobin for time-resolved immunofluorometric assay

Cited by 45 publications

References 27 publications

Gas-Phase Surface Engineering of Polystyrene Beads Used to Challenge Automated Particle Inspection Systems

Gas-Phase Surface Engineering of Polystyrene Beads Used to Challenge Automated Particle Inspection Systems

Hydrophobin as a Nanolayer Primer That Enables the Fluorinated Coating of Poorly Reactive Polymer Surfaces

Organic-inorganic Interfaces for a New Generation of Hybrid Biosensors

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