Hydrophilic‐hydrophobic microdomain surfaces having an ability to suppress platelet aggregation and their <i>in vitro</i> antithrombogenicity

Okano, Teruo; Aoyagi, Takaaki; Kataoka, Kazunori; Abe, Kazuhiko; Sakurai, Yasuhisa; Shimada, Masashi; Shinohara, Isao

doi:10.1002/jbm.820200707

Cited by 142 publications

(53 citation statements)

References 5 publications

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“…Because platelets spontaneously detach immediately after attachment to the surfaces, platelets seem to be suppressed from proceeding to the subsequent irreversible step. 17 When a polymer surface is in contact with blood, the surface is rapidly covered with plasma proteins before interacting with platelets. 18 Platelets therefore adhere to the surface of the blood protein layer which is formed on the polymer surface.…”

Section: Discussionmentioning

confidence: 99%

Two different types of nonthrombogenic surfaces: PEG suppresses platelet adhesion ATP-independently but HEMA-St block copolymer requires ATP consumption of platelets to prevent adhesion

Uchida

Yamato

Ito

et al. 2000

J. Biomed. Mater. Res.

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Poly(ethylene glycol) (PEG) and a hydrophobic-hydrophilic microdomain structured block copolymer comprising poly(2-hydroxyethyl methacrylate) and polystyrene (HEMA-St) have been reported to show good blood compatibility owing to inhibition of platelet activation. By using a computer-assisted novel technique to analyze platelet behavior on the surfaces, we found two different mechanisms to prevent platelet adhesion. Platelets were prevented from adhesion and spreading on the microdomain surface and retained cell movement for a long time. The platelet movement velocity was not significantly different between PEG-grafted surfaces and HEMA-St block copolymer-cast surfaces. However, platelet motion was qualitatively different. Platelets on HEMA-St block copolymer-cast surfaces moved with rolling, spinning, and vibrating, whereas platelet movement was limited to oscillatory vibration on PEG-grafted surfaces. When platelets were treated with NaN(3), an adenosine triphosphate (ATP) synthesis inhibitor, before contacting the surfaces, platelets movement velocity was decreased only on HEMA-St block copolymer-cast surfaces. Such an inhibitory effect was hardly observed with platelets on PEG-grafted surfaces. We propose two different mechanisms to prevent platelet adhesion onto surfaces. One is ATP-independent as observed with PEG, and the other is ATP-dependent for HEMA-St block copolymer, where platelets consume ATP to prevent adhesion.

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Section: Discussionmentioning

confidence: 99%

Two different types of nonthrombogenic surfaces: PEG suppresses platelet adhesion ATP-independently but HEMA-St block copolymer requires ATP consumption of platelets to prevent adhesion

Uchida

Yamato

Ito

et al. 2000

J. Biomed. Mater. Res.

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“…These block copolymers, also called SMAs, are blended in a reactive mixture with polyurethane polymer resins and coated on device surfaces, as was the case for the SMA dialysis catheter (MC) we used (US patent 6841255). SMAs improve biocompatibility by delaying contact activation and reducing thrombin-antithrombin complex generation [12], and diminish the thrombogenicity by hampering adhesion of platelets [22,23]. The SMA-coated catheters we used had a very smooth surface compared to the conventional catheters, and more importantly, this coating prevented the release of BaSO 4 by sealing the surface with a continuous and closed polymer film.…”

Section: Discussionmentioning

confidence: 99%

The role of polymer surface degradation and barium sulphate release in the pathogenesis of catheter-related infection

Verbeke

Haug

Dhondt

et al. 2009

Nephrology Dialysis Transplantation

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Background. Susceptibility to infection and thrombosis of intravascular catheters is increased by surface irregularities, which might be prevented by coating. Methods. BaSO4 release from conventional haemodialysis catheters (CC) and modified catheters (MC) which had been coated with a surface-modifying additive (SMA) was assessed in vivo and in vitro. For the in vivo part, patients were randomized to receive a temporary CC or MC, with crossover after 1 week. After retrieval, catheters were examined using scanning electron microscopy to assess surface integrity, and an in vitro model of catheter exposure to the bloodstream was used to evaluate surface morphology and susceptibility to bacterial adhesion and proliferation. Results. BaSO 4 moieties covered 14.7 ± 3.7% of the surface of unused CC. After in vivo use in 16 patients, 62.7 ± 32.9 × 10 3 holes/mm 2 were detected, indicating BaSO 4 detachment from 3.3 ± 1.7% of the catheter surface. No defects were observed in unused CC and in MC, whether used or unused. After incubation of four catheters (two of each type) with Staphylococcus epidermidis, the two degraded CC showed an immediate and strong bacterial growth as indicated by an increase in medium impedance of 0.512%/10 min compared to −0.021%/10 min in MC (P < 0.001). Conclusions. Short-term exposure of CC to the bloodstream causes BaSO 4 particle release, resulting in surface irregularities predisposing to bacterial proliferation. BaSO 4 release can be prevented by SMA coating.

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“…The aqueous solution of the salt of nylon 610 and sebacic acid corresponding to the amount of a,v-bisaminoisopropyl poly ( propylene oxide) ( diamino-PPO, M w Å 2000, Sun Techno Chemical Co. Ltd., Japan ) ( 25 wt %) or a,v-bisaminopropyl poly ( tetramethylene ox- 3 . S is the membrane area where V is the measured water flux ( mL/ h ) , S is (cm 2 ) and 10 cm 2 .…”

Section: Experimental Preparation Of Ppo-ny610 and Ptmo-ny610mentioning

confidence: 99%

Polyether-segmented nylon hemodialysis membranes. I. Preparation and permeability characteristics of polyether-segmented nylon 610 hemodialysis membrane

Seita

Mochizuki

Agawa

et al. 1997

J. Appl. Polym. Sci.

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ABSTRACT:The effect of the coagulation condition in the phase inversion method on the permeability characteristics of poly( propylene oxide ) or poly(tetramethylene oxide )-segmented nylon 610 (PPO-Ny610 or PTMO-Ny610 ) hemodialysis membranes, the stability of the membrane performance, and the mechanical strength were investigated. The polymers were dissolved in a solvent such as formic acid and methanol saturated with calcium chloride, and thus PPO-Ny610 and PTMO-Ny610 membranes were prepared using formic acid and a calcium chloride /methanol/water mixture as a polymer solvent and a coagulant, respectively. It is concluded that PPO-Ny610 membrane has better permeability characteristics than PTMO-Ny610 membrane, and possesses additional properties for hemodialysis membranes such as mechanical properties and permeability stability in the drying and sterilizing processes. Furthermore, the blood compatibilities of PPO-Ny610 and PTMO-Ny610 membranes were superior to regenerated cellulose membranes on the basis of the result of platelet adhesion test.

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Hydrophilic‐hydrophobic microdomain surfaces having an ability to suppress platelet aggregation and their in vitro antithrombogenicity

Cited by 142 publications

References 5 publications

Two different types of nonthrombogenic surfaces: PEG suppresses platelet adhesion ATP-independently but HEMA-St block copolymer requires ATP consumption of platelets to prevent adhesion

Two different types of nonthrombogenic surfaces: PEG suppresses platelet adhesion ATP-independently but HEMA-St block copolymer requires ATP consumption of platelets to prevent adhesion

The role of polymer surface degradation and barium sulphate release in the pathogenesis of catheter-related infection

Polyether-segmented nylon hemodialysis membranes. I. Preparation and permeability characteristics of polyether-segmented nylon 610 hemodialysis membrane

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