Hydrolytic enzyme screening and carotenoid production evaluation of halophilic archaea isolated from highly heavy metal-enriched solar saltern sediments

Baati, Houda; Siala, Mariem; Azri, Chafai; Ammar, Emna; Trigui, Mohamed

doi:10.1007/s42770-022-00855-6

Cited by 3 publications

(3 citation statements)

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“…In particular, Chlorella vulgaris has been found to contain significant amounts of pigments such as carotenes, lutein, chlorophylls, and vitamins such as vitamin C, all of which have antioxidant capacity [37,55,56]. The antioxidant effect of various haloarchaea has been studied and associated with the presence of carotenes [57,58]. While our results suggest that Halobacterium salinarum does not exhibit antioxidant activity, several studies have reported that the AE of Halobacterium salinarum showed significant antioxidant capacity [27,38].…”

Section: Discussioncontrasting

confidence: 50%

Extracts from Microalgae and Archaea from the Andalusian Coast: A Potential Source of Antiproliferative, Antioxidant, and Preventive Compounds

Luque,

Perazzoli,

Gómez-Villegas

et al. 2024

JMSE

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Marine and extreme environments harbor a huge diversity of microorganisms able to produce new bioactive metabolites with beneficial health effects. In this study, ethanol, aqueous, methanol, and acetone extracts and protein hydrolysates were obtained from five different microalgae species and two haloarchaea. An in vitro study of cytotoxicity, migration, angiogenic effect, antioxidant capacity, and modulation of detoxifying enzyme expression was carried out using resistant (HCT-15) and non-resistant (T84) colon cancer tumor lines. Our results showed that the aqueous extract of the microalga Chlorella sorokiniana induced the greatest cytotoxic effect in both cell lines, while the ethanolic extracts of the archaea Haloarcula hispanica and Halobacterium salinarum caused the greatest inhibition on the migratory capacity. Meanwhile, the protein hydrolyzate and the aqueous extract of the microalga Chlorella sorokiniana significantly protected cells against hydrogen peroxide damage. Moreover, the aqueous extracts of Haloarcula hispanica and Halobacterium salinarum resulted in inducing the greatest increase in the activity of the detoxifying enzymes enzyme quinone oxidoreductase and glutathione S-transferase. These preliminary results suggest that aqueous extracts of some microalgae and haloarchaea may be promising candidates for an adjuvant therapy against colorectal cancer. However, additional research is required to identify the active principles and elucidate the mechanisms of action involved.

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Section: Discussioncontrasting

confidence: 50%

Extracts from Microalgae and Archaea from the Andalusian Coast: A Potential Source of Antiproliferative, Antioxidant, and Preventive Compounds

Luque,

Perazzoli,

Gómez-Villegas

et al. 2024

JMSE

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“…Haloarchaea constitutes one of the most predominant groups of the microbial community present in hypersaline habitats (Baati et al 2022;Martínez et al 2022;Najjari et al 2015;Najjari et al 2021). Adaptations in such harsh environments render the genome of halophilic archaea abundant in many essential genes that are lacking in other microorganisms (Feng et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Physiological and genomic insights into abiotic stress of halophilic archaeon Natrinema altunense 4.1R isolated from a saline ecosystem of Tunisian desert

et al. 2023

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Halophilic archaea are polyextremophiles with the ability to withstand fluctuations in salinity, high levels of ultraviolet radiation, and oxidative stress, allowing them to survive in a wide range of environments and making them an excellent model for astrobiological research. Natrinema altunense 4.1R is a halophilic archaeon isolated from the endorheic saline lake systems, Sebkhas, located in arid and semi-arid regions of Tunisia. It is an ecosystem characterized by periodic flooding from subsurface groundwater and fluctuating salinities. Here, we assess the physiological responses and genomic characterization of N. altunense 4.1R to UV-C radiation, as well as osmotic and oxidative stresses. Results showed that the 4.1R strain is able to survive up to 36% of salinity, up to 180 J/m2 to UV-C radiation, and at 50 mM of H2O2, a resistance profile similar to Halobacterium salinarum, a strain often used as UV-C resistant model. In order to understand the genetic determinants of N. altunense 4.1R survival strategy, we sequenced and analyzed its genome. Results showed multiple gene copies of osmotic stress, oxidative stress, and DNA repair response mechanisms supporting its survivability at extreme salinities and radiations. Indeed, the 3D molecular structures of seven proteins related to responses to UV-C radiation (excinucleases UvrA, UvrB, and UvrC, and photolyase), saline stress (trehalose-6-phosphate synthase OtsA and trehalose-phosphatase OtsB), and oxidative stress (superoxide dismutase SOD) were constructed by homology modeling. This study extends the abiotic stress range for the species N. altunense and adds to the repertoire of UV and oxidative stress resistance genes generally known from haloarchaeon.

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“…Способность галофильных микроорганизмов существовать в засоленных средах обосновывается синтезом ферментов, которые повышают устойчивость культуры в кислой среде (Kumudha & Sarada, 2016;Рязанцев & Муратов, 2021). Данный механизм помогает микроводорослям поддерживать протекание биохимических процессов в клетках (Baati et al, 2022;Jungmann et al, 2022).…”

Section: Introductionunclassified

The Effect of Sodium Chloride on Biomass Growth and Protein Synthesis during Cultivation of Dunaliella salina Microalgae on a Nutrient Medium from Beet Molasses

Iksanov,

Kanarsky,

Kanarskaya

et al. 2023

jour

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Introduction. Currently, the applications of halophiles of the genus Halobacteriaceae are mainly limited to the use of β-carotene and hydrolases in the food and cosmetic industries. The expansion of halophile applications in biotechnology is based on the synthesis of effective and highly specific biocatalysts that can meet industrial needs. Halophiles are excellent sources of enzymes that are not only resistant to salt, but can also be effective in other extreme conditions. The resistance of halophiles to external factors is of increasing interest to them from biotechnologists, because they are the source of many BAS, and their habitat allows cultivation in non-sterile conditions. In this regard, it is of scientific and practical interest to determine the possibility of using D. salina bacteria in industry, which are recommended for wastewater treatment, expression of recombinant proteins, production of biofuels, production of natural polymer materials.Purpose. To study the effectiveness of cultivation of microalgae Dunaliella salina (D. salina) on a nutrient medium from molasses, experiments with additional addition of sodium chloride were carried out in this work.Materials and Methods. The review includes foreign articles published in English for the period 2010-2023. Search for scientific articles on suitable topics in the bibliographic databases Google Scholar, Scopus, Web of Science. When selecting publications for review, priority was given to highly cited sourcesResults. It was found that an increase in the cultivation temperature of microalgae from 5 to 25℃ favorably affects the growth characteristics of D. Salina culture: the specific growth rate increases, and the cell generation time decreases. When the content of sodium chloride in the nutrient medium is up to 5%, D. salina microalgae exhibit halotolerant properties. With an increase in the NaCl content in the nutrient medium up to 30%, D. salina culture exhibits halophilic properties. The best kinetic characteristics of the growth of D. salina culture are manifested with halophilic physiological activity. The yield of biomass of D. salina microalgae during cultivation on a nutrient medium without application and when introducing 5% sodium chloride into the nutrient medium from molasses is higher compared to the introduction of 15 and 30% sodium chloride into the nutrient medium. According to the results obtained, it can be said that an increase in the content of sodium chloride leads to an increase in the growth rate of the culture and the generation time, also in the values of 30% NaCl, a higher content of protein and β-fructosidase is manifested. With an increase in the duration of cultivation up to 240 h, a decrease in the pH of the nutrient medium from 7.04 to 4.70 is observed, which is due to the assimilation of mineral and organic nitrogen by microalgae. With an increase in the duration of cultivation of microalgae, there is an increase in the number of cells in the culture fluid up to 4 × 107 cl /ml, which at the same time synthesize the extracellular enzyme β-fructosidase, hydrolyzing sucrose.Conclusions. In view of the studies obtained, it is possible to indicate the possibility of using beet molasses as a source of carbon and substances that stimulate the growth of D.salina to produce biomass, protein substances and the enzyme β-fructosidase.

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Hydrolytic enzyme screening and carotenoid production evaluation of halophilic archaea isolated from highly heavy metal-enriched solar saltern sediments

Cited by 3 publications

References 68 publications

Extracts from Microalgae and Archaea from the Andalusian Coast: A Potential Source of Antiproliferative, Antioxidant, and Preventive Compounds

Extracts from Microalgae and Archaea from the Andalusian Coast: A Potential Source of Antiproliferative, Antioxidant, and Preventive Compounds

Physiological and genomic insights into abiotic stress of halophilic archaeon Natrinema altunense 4.1R isolated from a saline ecosystem of Tunisian desert

The Effect of Sodium Chloride on Biomass Growth and Protein Synthesis during Cultivation of Dunaliella salina Microalgae on a Nutrient Medium from Beet Molasses

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