Hydrolysis of isoflavone in black soy milk using cellulose bead as enzyme immobilizer

Abstract

The establishment of a catalytic system to enrich isoflavone aglycones in black soybean milk was investigated in this study. Beta-glucosidase, which was covalently immobilized onto cellulose beads, exhibited a significant efficiency for the conversion of 4-nitrophenyl β-d-glucuronide to p-nitrophenol over the sol-gel method. The Michaelis constant (K) of the cellulose bead enzymatic system was determined to be 1.50±0.10 mM. Operational reusability of the cellulose bead enzymatic system was justified for more than 10 batch reactions in black soy milk. Moreover, the storage stability verification indicated that the cellulose bead catalytic system was able to sustain its highest catalytic activity for 10 days. High-performance liquid chromatography results demonstrated that this enzymatic system required only 30 minutes to achieve complete isoflavone deglycosylation, and the aglycone content in the total isoflavones in black soy milk was enriched by 67% within 30 minutes by the cellulose bead enzymatic system.

Lipid-Lowering Effects and Intestinal Transport of Polyphenol Extract from Digested Buckwheat in Caco-2/HepG2 Coculture Models

“…It was also illustrated in previous studies that deglycosylation occurs only via colonic enzymes for its hydrolysis. 31,32 Although hydroxycinnamic acid and quercetin are the most abundant in BW extracts and possess high bioavailability, their rate of BL/AP uptake in the Caco-2/HepG2 coculture model was less than 50%. The main reason for this phenomenon is that the presence of a catechol-type structure, 2,3-double bond, and 3-and 5-hydroxyl groups in the aromatic ring weakened hydroxycinnamic acid stability in DMEM.…”

Biocompatible natural deep eutectic solvent-based extraction and cellulolytic enzyme-mediated transformation of Pueraria mirifica isoflavones: a sustainable approach for increasing health-bioactive constituents

“…Cellulolytic enzymes did not change the level of PUE in the reaction mixture because PUE contains a C-glycosidic bond. β-Glucosidase is reactive toward the β-O-glycosidic bonds of DZ and GT (Chen et al 2016). Thus, the enzymes cannot cleave PUE with C-glycosidic bonds.…”

Lactobacillus plantarumTWK10‐fermented soymilk improves cognitive function in type 2 diabetic rats

“…8 Moreover, Chen et al showed that the cellulose bead enzymatic system can enrich the aglycone content in black soy milk. 9 Food waste utilized as Lactobacillus cultivation medium can enhance the antitumor and immunomodulatory properties. 10 In fermented soybean products, the high content of aglycone isoflavones prevents high fat diet-induced fat accumulation and improves antioxidant capacities in vivo.…”

“…Moreover, Chen et al . showed that the cellulose bead enzymatic system can enrich the aglycone content in black soy milk 9 . Food waste utilized as Lactobacillus cultivation medium can enhance the antitumor and immunomodulatory properties 10 .…”