Hydrogen-rich saline solution attenuates renal ischemia–reperfusion injury

Shingu, Chihiro; Koga, Hironori; Hagiwara, Satoshi; Matsumoto, Shigekiyo; Goto, Koji; Yokoi, Isao; Noguchi, Takayuki

doi:10.1007/s00540-010-0942-1

Cited by 67 publications

(50 citation statements)

References 24 publications

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“…Our present study found that intraperitoneal injection of hydrogen-rich saline significantly attenuated I/R-induced apoptosis, MPO activity, MDA, 8-OhdG, TNF-a, IL-1b, and IL-6 levels and increased SOD and CAT activities in the renal tissues compared with those in I/R plus physiologic saline treatment rats, which was in consistent with a recent study that demonstrated a decrease in the serum level of 8-OhdG after continuous intravenous administration of hydrogen-rich saline [9].…”

Section: Discussionsupporting

confidence: 93%

Hydrogen-Rich Saline Protects Against Renal Ischemia/Reperfusion Injury in Rats

Wang

Liu

et al. 2011

Journal of Surgical Research

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Section: Discussionsupporting

confidence: 93%

Hydrogen-Rich Saline Protects Against Renal Ischemia/Reperfusion Injury in Rats

Wang

Liu

et al. 2011

Journal of Surgical Research

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“…Renal ischemia/reperfusion (I/R) is a significant cause of acute kidney injury (AKI) and an inevitable consequence of several operations, including the treatment of suprarenal aortic aneurysms, partial nephrectomy, surgical revascularization of the renal artery and renal transplantation [1]. Renal ischemia-reperfusion injury (IRI) is clearly a leading clinical problem in native kidneys as well as in the setting of renal transplantation, but the pathogenesis of renal IRI remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Up-Regulation of HMGB1 Exacerbates Renal Ischemia-Reperfusion Injury by Stimulating Inflammatory and Immune Responses through the TLR4 Signaling Pathway in Mice

Chen¹,

Liu²,

Zhou³

et al. 2017

Cell Physiol Biochem

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Background/Aims: The aim of this study was to elucidate how high-mobility group box 1 (HMGB1) exacerbates renal ischemic-reperfusion injury (IRI) by inflammatory and immune responses through the toll-like receptor 4 (TLR4) signaling pathway. Methods: A total of 30 wild-type (WT) mice and 30 TLR4 knockout (TLR4 -/-) mice were selected and then randomly assigned to the Sham, I/R or HMGB1 groups. The serum and kidney tissues of all mice were collected 24 h after the perfusion. The fully automatic biochemical detector and ELISA were applied to determine the blood urea nitrogen (BUN) and serum creatinine (Scr) levels, and TNF-α, IL-1β, IL-6, IFN-γ and IL-10 levels, respectively. HE staining was used to evaluate kidney tissue damage, immunofluorescence and immunohistochemical staining were performed to observe CD68 and MPO cell infiltration, and flow cytometry was applied to detect immune cells. qRT-PCR and Western blotting were used to detect the expressions of TLR signaling pathwayrelated genes and proteins, respectively. Results: Compared with the Sham group, the levels of BUN, Scr, TNF-α, IL-1β, IL-6, IFN-γ and IL-10, kidney tissue damage score, CD68 and MPO cell infiltration, the numbers of immune cells, and the expressions of TLR signaling pathwayrelated genes and proteins in the I/R and HMGB1 groups were significantly up-regulated. In the I/R and HMGB1 groups, the levels of BUN and Scr, TNF-α, IL-1β, IL-6 and IFN-γ, kidney tissue damage score, CD68 and MPO cell infiltration, immune cell numbers, and TLR signaling pathway-related gene and protein expressions in the WT mice were all higher than those in the TLR4 -/-mice, but IL-10 level was significantly lower. Similarly, all aforementioned indexes but

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“…Renal ischemia/reperfusion (I/R) injuries are the major causes of acute renal failure and may be involved in the development and progression of certain forms of chronic kidney disease (1). Ischemia is associated with oxidative stress and apoptosis.…”

Section: Introductionmentioning

confidence: 99%

Effects of quercetin on apoptosis, NF-κB and NOS gene expression in renal ischemia/reperfusion injury

Kinaci

Erkasap

Küçük

et al. 2011

Experimental and Therapeutic Medicine

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Abstract. The aim of this study was to investigate the effects of quercetin on nitric oxide synthase (NOS), nuclear factor-κB (NF-κB) and apoptosis in renal ischemia/reperfusion (I/R) injury in rats. A total of 42 Sprague-Dawley rats were divided into three groups. The control, I/R and I/R+quercetin (I/R+Q) groups were treated with quercetin (50 mg/kg intraperitoneal) 1 h prior to the induction of ischemia. Tissue malondialdehyde (MDA) and glutathione (GSH) levels were determined by high-performance liquid chromatography (HPLC). p53, endothelial NOS (eNOS) and NF-κB expression were assessed immunohistochemically, and apoptosis assesment was performed using terminal deoxy nucleotidyl transferase dUTP nick end-labeling (TUNEL) assay. The mRNA levels of inducible NOS (iNOS) in renal tissue were determined by realtime polymerase chain reaction (RT-PCR). MDA levels were significantly decreased in the quercetin group compared to the I/R group. However, GSH levels were significantly increased with quercetin treatment in the I/R group. Histological results, the number of apoptotic and p53-positive cells, NF-κB and eNOS expression levels were significantly decreased in the quercetin treatment group compared to the I/R group. iNOS gene expression increased in the I/R group, but no significant difference was found between the I/R and quercetin treatment groups. Therefore, quercetin not only has antioxidant and antiapoptotic activities, but also has an inhibitory effect on eNOS and NF-κB for renal tissue protection during I/R injury in rats. Therefore, quercetin may be a promising renoprotective therapeutic agent.

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Hydrogen-rich saline solution attenuates renal ischemia–reperfusion injury

Cited by 67 publications

References 24 publications

Hydrogen-Rich Saline Protects Against Renal Ischemia/Reperfusion Injury in Rats

Hydrogen-Rich Saline Protects Against Renal Ischemia/Reperfusion Injury in Rats

Up-Regulation of HMGB1 Exacerbates Renal Ischemia-Reperfusion Injury by Stimulating Inflammatory and Immune Responses through the TLR4 Signaling Pathway in Mice

Effects of quercetin on apoptosis, NF-κB and NOS gene expression in renal ischemia/reperfusion injury

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