volume 16, issue 1, P10-17 1994
DOI: 10.1016/0141-0229(94)90103-1
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Min Zhang, Henry Y. Wang

Abstract: A new method has been developed to prepare intact cell systems to produce hydrogen peroxide (H202) using methylotrophic yeast Pichia pastoris. The high alcohol oxidase (EC 1.1.3.13; AOXase) content in the living P. pastoris cells is always associated with high activity of catalase (about 200-to 400-fold higher than that of AOXase) to protect the cells from oxidative damage from 1-1202. To obtain catalasefree intact cells, a combination of a cationic detergent, cetyldimethylbenzyl-ammonium chloride (Cation M2),…

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