Hydrogen peroxide-induced neuronal apoptosis is associated with inhibition of protein phosphatase 2A and 5, leading to activation of MAPK pathway

Chen, Long; Liu, Lei; Yin, Jun; Luo, Yan; Huang, Shile

doi:10.1016/j.biocel.2008.10.029

Cited by 210 publications

(197 citation statements)

References 43 publications

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“…This suggested that • OH-induced cytochrome c release and ROS generation in fish erythrocytes. This is consistent with the report that H 2 O 2 induces cytochrome c release in HeLa cells [36] and ROS production in rat neuronal cells [78]. The cytochrome c release and ROS generation may be related to mitochondrion in fish erythrocytes.…”

Section: • Oh-induced Apoptosis In Fish Erythrocytessupporting

confidence: 93%

The metabolites of glutamine prevent hydroxyl radical-induced apoptosis through inhibiting mitochondria and calcium ion involved pathways in fish erythrocytes

Jiang

Liu

et al. 2016

Free Radical Biology and Medicine

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a b s t r a c tThe present study explored the apoptosis pathways in hydroxyl radicals ( • OH)-induced carp erythrocytes. Carp erythrocytes were treated with the caspase inhibitors in physiological carp saline (PCS) or Ca 2 þ -free PCS in the presence of 40 μM FeSO 4 /20 μM H 2 O 2 . The results showed that the generation of reactive oxygen species (ROS), the release of cytochrome c and DNA fragmentation were caspase-dependent, and Ca 2 þ was involved in calpain activation and phosphatidylserine (PS) exposure in • OH-induced carp erythrocytes. Moreover, the results suggested that caspases were involved in PS exposure, and Ca 2 þ was involved in DNA fragmentation in • OH-induced fish erythrocytes. These results demonstrated that there might be two apoptosis pathways in fish erythrocytes, one is the caspase and cytochrome c-dependent apoptosis that is similar to that in mammal nucleated cells, the other is the Ca 2 þ -involved apoptosis that was similar to that in mammal non-nucleated erythrocytes. So, fish erythrocytes may be used as a model for studying oxidative stress and apoptosis in mammal cells. Furthermore, the present study investigated the effects of glutamine (Gln)'s metabolites [alanine (Ala), citrulline (Cit), proline (Pro) and their combination (Ala 10 Pro 4 Cit 1 )] on the pathways of apoptosis in fish erythrocytes. The results displayed that Ala, Cit, Pro and Ala 10 Pro 4 Cit 1 effectively suppressed ROS generation, cytochrome c release, activation of caspase-3, caspase-8 and caspase-9 at the physiological concentrations, prevented Ca 2 þ influx, calpain activation, PS exposure, DNA fragmentation and the degradation of the cytoskeleton and oxidation of membrane and hemoglobin (Hb) and increased activity of anti-hydroxyl radical (AHR) in • OH-induced carp erythrocytes. Ala 10 Pro 4 Cit 1 produced a synergistic effect of inhibited oxidative stress and apoptosis in fish erythrocytes. These results demonstrated that Ala, Cit, Pro and their combination can protect mammal erythrocytes and nucleated cells against oxidative stress and apoptosis. The studies supported the use of Gln, Ala, Cit and Pro as oxidative stress and apoptosis inhibitors in mammal cells and the hypothesis that the inhibited effects of Gln on oxidative stress and apoptosis are at least partly dependent on that of its metabolites in mammalian.

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Section: • Oh-induced Apoptosis In Fish Erythrocytessupporting

confidence: 93%

The metabolites of glutamine prevent hydroxyl radical-induced apoptosis through inhibiting mitochondria and calcium ion involved pathways in fish erythrocytes

Jiang

Liu

et al. 2016

Free Radical Biology and Medicine

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“…We have detected cleaved caspase‐3, caspase‐6, and, caspase‐7 in the H 2 O 2 ‐treated cells, but found antiapoptotic effects of Cheongja#3 by the rescued cleaved caspases. Mitogen‐activated protein kinase pathway, especially phosphorylated p38 and JNK, is en route to apoptosis in response to environmental stress such as ROS (Chen, Liu, Yin, Luo, & Huang, 2009; Sui et al., 2014). Phosphorylated JNK and phosphorylated p38 protein expressions were increased upon treatment of H 2 O 2 as other studies have shown.…”

Section: Discussionmentioning

confidence: 99%

Black soybeans protect human keratinocytes from oxidative stress‐induced cell death

Yoon

Lee

Song

et al. 2018

Food Science & Nutrition

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Black soybeans are functional foods containing a variety of bioactives such as isoflavones, carotenoids, tocopherols, phenolic acid as well as anthocyanins. Here, we examined whether Cheongja#3 black soybean extract has a protective effect on oxidative stress‐induced cell death in human keratinocytes HaCaT. First, we identified fat‐soluble bioactives in three varieties of soybean extracts (Saedanbaek, Daechan, and Cheongja#3). In particular, black soybean Cheongja#3 had high amounts of lutein than other varieties. We demonstrated that Cheongja#3 extract reduced intracellular reactive oxygen species levels in HaCaT cells. Furthermore, Cheongja#3 protected cells from hydrogen peroxide (H2O2)‐induced oxidative stress and triggered cell death determined by cell viabilities and apoptotic caspase activities. Next, we identified the underlying mechanism is due to increased Nrf2 antioxidant system by Cheongja#3, thus increasing the expression of heme oxygenases (HO)‐1. These results indicated that Cheongja#3 soybean extract has protective role against oxidative stress by upregulating the Nrf‐2 antioxidant system in human keratinocyte HaCaT cells.

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“…The data from other groups (Kim et al, 2005) suggested that ROS activation of the mitogen activated protein kinase (MAPK) cascades might be a common mechanism by which oxidative stress induces cell death. Chen et al (2009) identified that oxidative stress activates the MAPK pathway by induction of ROS and inhibition of PP 2A leading to apoptosis of neuronal cells. MC-RR can strongly inhibit PP 1 and PP 2A (Gehringer, 2004).…”

Section: Resultsmentioning

confidence: 99%

Mitochondrial electron transport chain is involved in microcystin-RR induced tobacco BY-2 cells apoptosis

Huang

Liu

2014

Journal of Environmental Sciences

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Hydrogen peroxide-induced neuronal apoptosis is associated with inhibition of protein phosphatase 2A and 5, leading to activation of MAPK pathway

Cited by 210 publications

References 43 publications

The metabolites of glutamine prevent hydroxyl radical-induced apoptosis through inhibiting mitochondria and calcium ion involved pathways in fish erythrocytes

The metabolites of glutamine prevent hydroxyl radical-induced apoptosis through inhibiting mitochondria and calcium ion involved pathways in fish erythrocytes

Black soybeans protect human keratinocytes from oxidative stress‐induced cell death

Mitochondrial electron transport chain is involved in microcystin-RR induced tobacco BY-2 cells apoptosis

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