Hydrogen-Peroxide-Enhanced Nonthermal Plasma Effluent for Biomedical Applications

Gołkowski, Mark; Golkowski, C.; Leszczynski, Jori K; Plimpton, S. Reed; Masłowski, Piotr; Foltynowicz, Aleksandra; Ye, Jun; McCollister, Bruce D.

doi:10.1109/tps.2012.2200910

Cited by 52 publications

(47 citation statements)

References 27 publications

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“…Several nonthermal plasma generators display higher levels of hydroxyl radicals, compared with non-water vapor plasma discharges, when supplied with water vapor [18,19,25]. Alteration of the amount of oxygen in the starting plasma, gaseous medium has been shown to affect the amount of nitrogen oxide during association with nitrogen.…”

Section: Plasma Spectra Characteristicsmentioning

confidence: 99%

“…Several recent studies have referenced the use of water vapor in the generation of nonthermal plasma. These studies have identified discharge spectra profiles, composed of elevated hydroxyl radicals and hydrogen peroxide [18,19]. This current paper does not discuss the use of humidified air or water vapor as a source of plasma starting material.…”

Section: Introductionmentioning

confidence: 99%

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Characterization, Properties and Applications of Nonthermal Plasma: A Novel Pulsed-Based Option

Ferrell¹,

Aleks²,

Galov³

et al. 2013

J Biotechnol Biomaterial

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The glow discharge from a novel spark-based nonthermal plasma generator is described and characterized using spectrophotometry, radiometry and gas detectors. Spectral information identified ultraviolet radiation within the plasma discharge. High levels of reactive oxygen and nitrogenous species were also detected within the analyzed plasma. The plasma discharge is found to have a significant germicidal effect on Gram-positive bacteria, independent of temperature increase. The ultraviolet radiation and ionized gaseous species are found to contribute to this observed effect, synergistically. Plasma filtration reduced germicidal survivability significantly. The liquid environment in which bacterial cells were exposed to the nonthermal plasma also was important, when determining the efficacy of nonthermal plasma, as a germicidal agent. Staphylococcus aureus cultures exposed to nonthermal plasma for two minutes within low nutrient sources, such as water or phosphate-buffered saline, had very low survival. Cultures exposed to the same plasma interval in nutrient rich liquids tolerated the plasma exposure better, and survived at higher numbers. This plasma discharge is highly bactericidal, due to the high concentrations of known antimicrobial agents, but its effectiveness is subject to a synergistic effect, that depends upon the surrounding environment.

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Section: Plasma Spectra Characteristicsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization, Properties and Applications of Nonthermal Plasma: A Novel Pulsed-Based Option

Ferrell¹,

Aleks²,

Galov³

et al. 2013

J Biotechnol Biomaterial

View full text Add to dashboard Cite

show abstract

“…In many instances, hydrogen peroxide (H 2 O 2 ) was an important mediator in these in vitro experiments [53][54][55]. H 2 O 2 is not necessarily toxic by itself but rather exerts its biological effects through secondary processes, for example, Fenton reaction [56], by acting as substrate for oxygenases [57], and in redox signaling events enabling the translation of redox events into distinct biological responses [58].…”

Section: Introductionmentioning

confidence: 99%

A Comparison of Floating-Electrode DBD and kINPen Jet: Plasma Parameters to Achieve Similar Growth Reduction in Colon Cancer Cells Under Standardized Conditions

Bekeschus

Lin

Fridman

et al. 2017

Plasma Chem Plasma Process

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A comparative study of two plasma sources (floating-electrode dielectric barrier discharge, DBD, Drexel University; atmospheric pressure argon plasma jet, kINPen, INP Greifswald) on cancer cell toxicity was performed. Cell culture protocols, cytotoxicity assays, and procedures for assessment of hydrogen peroxide (H 2 O 2 ) were standardized between both labs. The inhibitory concentration 50 (IC50) and its corresponding H 2 O 2 deposition was determined for both devices. For the DBD, IC50 and H 2 O 2 generation were largely dependent on the total energy input but not pulsing frequency, treatment time, or total number of cells. DBD cytotoxicity could not be replicated by addition of H 2 O 2 alone and was inhibited by larger amounts of liquid present during the treatment. Jet plasma toxicity depended on peroxide generation as well as total cell number and amount of liquid. Thus, the amount of liquid present during plasma treatment in vitro is key in attenuating short-lived species or other physical effects from plasmas. These in vitro results suggest a role of liquids in or on tissues during plasma treatment in a clinical setting. Additionally, we provide a platform for correlation between different plasma sources for a predefined cellular response.

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“…Golkowski et al . () investigated the antibiofilm effect of hydrogen peroxide‐enhanced DBD effluent against E. coli biofilms. The discharge was physically removed from the disinfection zone (3 m physical distance), and plasma‐induced free radicals were delivered through an air stream.…”

Section: Inactivation Of Biofilm‐associated Bacteria By Ntpsmentioning

confidence: 99%

Potential applications of nonthermal plasmas against biofilm-associated micro-organisms in vitro

Puligundla

Mok

2017

J Appl Microbiol

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Summary Biofilms as complex microbial communities attached to surfaces pose several challenges in different sectors, ranging from food and healthcare to desalination and power generation. The biofilm mode of growth allows microorganisms to survive in hostile environments and biofilm cells exhibit distinct physiology and behaviour in comparison with their planktonic counterparts. They are ubiquitous, resilient and difficult to eradicate due to their resistant phenotype. Several chemical‐based cleaning and disinfection regimens are conventionally used against biofilm‐dwelling micro‐organisms in vitro. Although such approaches are generally considered to be effective, they may contribute to the dissemination of antimicrobial resistance and environmental pollution. Consequently, advanced green technologies for biofilm control are constantly emerging. Disinfection using nonthermal plasmas (NTPs) is one of the novel strategies having a great potential for control of biofilms of a broad spectrum of micro‐organisms. This review discusses several aspects related to the inactivation of biofilm‐associated bacteria and fungi by different types of NTPs under in vitro conditions. A brief introduction summarizes prevailing methods in biofilm inactivation, followed by introduction to gas discharge plasmas, active plasma species and their inactivating mechanism. Subsequently, significance and aspects of NTP inactivation of biofilm‐associated bacteria, especially those of medical importance, including opportunistic pathogens, oral pathogenic bacteria, foodborne pathogens and implant bacteria, are discussed. The remainder of the review discusses majorly about the synergistic effect of NTPs and their activity against biofilm‐associated fungi, especially Candida species.

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Hydrogen-Peroxide-Enhanced Nonthermal Plasma Effluent for Biomedical Applications

Cited by 52 publications

References 27 publications

Characterization, Properties and Applications of Nonthermal Plasma: A Novel Pulsed-Based Option

Characterization, Properties and Applications of Nonthermal Plasma: A Novel Pulsed-Based Option

A Comparison of Floating-Electrode DBD and kINPen Jet: Plasma Parameters to Achieve Similar Growth Reduction in Colon Cancer Cells Under Standardized Conditions

Potential applications of nonthermal plasmas against biofilm-associated micro-organisms in vitro

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