Hydrogel Encapsulation: Taking the Therapy of Mesenchymal Stem Cells and Their Derived Secretome to the Next Level

Huang, Yingna; Li, Xin; Yang, Lina

doi:10.3389/fbioe.2022.859927

Cited by 17 publications

(19 citation statements)

References 70 publications

(63 reference statements)

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“…Therefore, tissue engineering strategies for designing an effective biomaterial for vocal fold treatment have been highlighted to facilitate local healing and restore their native function 12 . An example is the use of mesenchymal stem cells (MSCs) or secretome encapsulation in hydrogel to promote native tissue regeneration and reduce the local immune system 13 . With that, current study serves as a preliminary study to develop a hydrogel which has potential for cell encapsulation in future work, in providing regenerative properties.…”

Section: Introductionmentioning

confidence: 99%

In vitro evaluation of genipin-crosslinked gelatin hydrogels for vocal fold injection

Lokanathan

Fauzi

et al. 2023

Sci Rep

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Glottic insufficiency is one of the voice disorders affecting all demographics. Due to the incomplete closure of the vocal fold, there is a risk of aspiration and ineffective phonation. Current treatments for glottic insufficiency include nerve repair, reinnervation, implantation and injection laryngoplasty. Injection laryngoplasty is favored among these techniques due to its cost-effectiveness and efficiency. However, research into developing an effective injectable for the treatment of glottic insufficiency is currently lacking. Therefore, this study aims to develop an injectable gelatin (G) hydrogel crosslinked with either 1-ethyl-3-(3-dimethylaminpropyl)carbodiimide hydrochloride) (EDC) or genipin (gn). The gelation time, biodegradability and swelling ratio of hydrogels with varying concentrations of gelatin (6–10% G) and genipin (0.1–0.5% gn) were investigated. Some selected formulations were proceeded with rheology, pore size, chemical analysis and in vitro cellular activity of Wharton's Jelly Mesenchymal Stem Cells (WJMSCs), to determine the safety application of the selected hydrogels, for future cell delivery prospect. 6G 0.4gn and 8G 0.4gn were the only hydrogel groups capable of achieving complete gelation within 20 min, exhibiting an elastic modulus between 2 and 10 kPa and a pore size between 100 and 400 μm. Moreover, these hydrogels were biodegradable and biocompatible with WJMSCs, as > 70% viability were observed after 7 days of in vitro culture. Our results suggested 6G 0.4gn and 8G 0.4gn hydrogels as potential cell encapsulation injectates. In light of these findings, future research should focus on characterizing their encapsulation efficiency and exploring the possibility of using these hydrogels as a drug delivery system for vocal fold treatment.

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Section: Introductionmentioning

confidence: 99%

In vitro evaluation of genipin-crosslinked gelatin hydrogels for vocal fold injection

Lokanathan

Fauzi

et al. 2023

Sci Rep

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“…Regarding cell therapy, hydrogels provide clear advantages to conventional culture. Their biophysical characteristics can be easily adjusted, other external physical stimuli (such as cyclic stretch) can be applied, are used as bioinks for bioprinting, and can be used as vehicle facilitating MSCs engraftment and viability after transplantation [12,13,24]. This work reveals that L-HG is also an important source of bioactive proteins and derived vesicles that can modulate multiple cellular mechanisms.…”

Section: Discussionmentioning

confidence: 94%

“…In a recent study, hydrogel-encapsulated MSCs could further alleviate acute lung injury, increasing the expression of several growth factors and interleukin-10 [12]. In addition, hydrogel-encapsulated MSCs showed better cell survival and could increase their engraftment in the injured tissue [13]. Thus, biomechanical preconditioning of cultured MSCs in physiomimetic hydrogels is a promising strategy to improve cell therapy in future clinical trials.…”

Section: Introductionmentioning

confidence: 99%

Lung Extracellular Matrix Hydrogels-Derived Vesicles Contribute to Epithelial Lung Repair

et al. 2022

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The use of physiomimetic decellularized extracellular matrix-derived hydrogels is attracting interest since they can modulate the therapeutic capacity of numerous cell types, including mesenchymal stromal cells (MSCs). Remarkably, extracellular vesicles (EVs) derived from MSCs display similar functions as their parental cells, mitigating tissue damage in lung diseases. However, recent data have shown that ECM-derived hydrogels could release other resident vesicles similar to EVs. Here, we aim to better understand the contribution of EVs and ECM-vesicles released from MSCs and/or lung-derived hydrogel (L-HG) in lung repair by using an in vitro lung injury model. L-HG derived-vesicles and MSCs EVs cultured either in L-HG or conventional plates were isolated and characterized. The therapeutic capacity of vesicles obtained from each experimental condition was tested by using an alveolar epithelial wound-healing assay. The number of ECM-vesicles released from acellular L-HG was 10-fold greater than EVs from conventional MSCs cell culture revealing that L-HG is an important source of bioactive vesicles. MSCs-derived EVs and L-HG vesicles have similar therapeutic capacity in lung repair. However, when wound closure rate was normalized by total proteins, the MSCs-derived EVs shows higher therapeutic potential to those released by L-HG. The EVs released from L-HG must be considered when HG is used as substrate for cell culture and EVs isolation.

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“…In order to overcome limitations associated with 2D culture, 3D cell culture techniques have been developed in the last decades that aim to recapitulate aspects of in vivo microenvironment. − Studies have shown that physiological functionalities and characteristics of MSCs can be maintained to a greater extent if cells are cultured in suitable 3D systems. − The suitability of such systems is highly dependent on employed biomaterials and the associated biophysical properties, which ultimately affect adherence, proliferation, differentiation, and migration of MSCs. − In this context, hydrogels have attracted attention in regenerative medicine due to their capacity to mimic aspects of the extracellular matrix (ECM) and thus provide a more in vivo -like microenvironment to the embedded cells during in vitro culture . Several types of synthetic and natural origin hydrogels, such as hyaluronic acid, alginate, collagen, gelatin, poly(ethylene glycol), and poly(lactic- co -glycolic acid) have been studied for cultivation of MSCs in 3D .…”

Section: Introductionmentioning

confidence: 99%

“… 18 − 20 In this context, hydrogels have attracted attention in regenerative medicine due to their capacity to mimic aspects of the extracellular matrix (ECM) and thus provide a more in vivo -like microenvironment to the embedded cells during in vitro culture. 21 Several types of synthetic and natural origin hydrogels, such as hyaluronic acid, alginate, collagen, gelatin, poly(ethylene glycol), and poly(lactic- co -glycolic acid) have been studied for cultivation of MSCs in 3D. 22 Hydrogels for biomedical applications are often associated with certain drawbacks, such as low mechanical stability, high variability, use of chemical cross-linking agents or irradiation and manufacturing costs.…”

Section: Introductionmentioning

confidence: 99%

Hydrogels from TEMPO-Oxidized Nanofibrillated Cellulose Support In Vitro Cultivation of Encapsulated Human Mesenchymal Stem Cells

Nikolits

Radwan

Liebner

et al. 2023

ACS Appl. Bio Mater.

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Mesenchymal stem cells (MSCs) are the most prominent type of adult stem cells for clinical applications. Three-dimensional (3D) cultivation of MSCs in biomimetic hydrogels provides a more physiologically relevant cultivation microenvironment for in vitro testing and modeling, thus overcoming the limitations of traditional planar cultivation methods. Cellulose nanofibers are an excellent candidate biomaterial for synthesis of hydrogels for this application, due to their biocompatibility, tunable properties, availability, and low cost. Herein, we demonstrate the capacity of hydrogels prepared from 2,2,6,6tetramethylpiperidine-1-oxyl -oxidized and subsequently individualized cellulose-nanofibrils to support physiologically relevant 3D in vitro cultivation of human MSCs at low solid contents (0.2−0.5 wt %). Our results show that MSCs can spread, proliferate, and migrate inside the cellulose hydrogels, while the metabolic activity and proliferative capacity of the cells as well as their morphological characteristics benefit more in the lower bulk cellulose concentration hydrogels.

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Hydrogel Encapsulation: Taking the Therapy of Mesenchymal Stem Cells and Their Derived Secretome to the Next Level

Cited by 17 publications

References 70 publications

In vitro evaluation of genipin-crosslinked gelatin hydrogels for vocal fold injection

In vitro evaluation of genipin-crosslinked gelatin hydrogels for vocal fold injection

Lung Extracellular Matrix Hydrogels-Derived Vesicles Contribute to Epithelial Lung Repair

Hydrogels from TEMPO-Oxidized Nanofibrillated Cellulose Support In Vitro Cultivation of Encapsulated Human Mesenchymal Stem Cells

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