volume 16, issue 8, P688-695 1994
DOI: 10.1016/0141-0229(94)90091-4
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B. Amos, M. Al-Rubeai, A.N. Emery

Abstract: Hybridoma cells were grown in perfusion culture using a stirred reactor within which a tubular membrane was suspended. Nutrient and product flows through the membrane to and from the culture environment occurred by diffusion processes alone. A mathematical model of the transfer and reaction process enabled both the characterization of a membrane mass transfer coefficient and the prediction of the maximum cell number achievable under set conditions. Steady states in cell concentration were observed for a range …

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