Hybrid necrosis as a barrier to gene transfer in hexaploid winter wheat × triticale crosses

Bizimungu, Benoît; Collin, J.; Comeau, A.; St-Pierre, C.

doi:10.4141/p96-185

Cited by 15 publications

(12 citation statements)

References 19 publications

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“…Therefore, although outcrossing with common wheat where triticale was the male parent occurred at a higher frequency, these outcrosses rarely produced viable seed. With the exception of the single emergent F 1 , these results support previous observations that hybrid seed produced from crosses between triticale and common wheat are only viable when triticale acts as the female parent in the cross (Bizimungu et al, 1998;Chaubey and Khanna, 1986;Khanna, 1990;Nkongolo et al, 1991). While crossability between triticale and common wheat is minimal, the scale of wheat acreage and the importance of this crop suggest testing should continue.…”

Section: Discussionsupporting

confidence: 77%

“…In contrast, emergence of 89TT108-durum wheat F 1 hybrids was 41% where triticale was the female parent, but no AC Alta-durum wheat F 1 hybrids emerged. A genetic component to crossability has been established in previous research (Bizimungu et al, 1998;Guedes-Pinto et al, 2001;Lelley, 1992;Lima-Brito and Guedes-Pinto, 1998;Nkongolo et al, 1991) and these results are not unexpected. Observations that outcrossing is influenced by differences in cultivars as well as environmental factors suggest that it may be advisable to conduct tier 2 testing with more cultivars in several environments.…”

Section: Discussionmentioning

confidence: 49%

“…Triticale was previously reported to outcross with common wheat (Triticum aestivum; 2n = 6x = 42; AABBDD) and rye (Chaubey and Khanna 1986;Guedes-Pinto et al, 2001;Lelley, 1992), though crossability was higher with wheat than with rye (Lelley, 1992). Crosses between triticale and common wheat where triticale was the female parent (ABR × ABD) produced viable F 1 seed, but the reciprocal cross (ABD × ABR) produced non-viable seed (Bizimungu et al, 1998;Chaubey and Khanna, 1986;Khanna, 1990;Nkongolo et al, 1991). Although the seed is non-viable, common wheat × triticale (ABD × ABR) hybrids can be obtained using in vitro embryo rescue (Kapila and Sethi, 1993).…”

Section: Introductionmentioning

confidence: 99%

“…Although the seed is non-viable, common wheat × triticale (ABD × ABR) hybrids can be obtained using in vitro embryo rescue (Kapila and Sethi, 1993). Hybridization was influenced by the genotypes of the parents used in the interspecific cross (Bizimungu et al, 1998;Guedes-Pinto et al, 2001;Lelley, 1992;Lima-Brito and Guedes-Pinto, 1998;Nkongolo et al, 1991). In addition to genetic factors, observations of variation in hybridization success in different years and at different temperatures suggest an environmental influence on hybridization (Guedes-Pinto et al, 2001;Nkongolo et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of crossability between triticale (XTriticosecaleWittmack) and common wheat, durum wheat and rye

Hills¹,

Hall²,

Messenger³

et al. 2007

Environ. Biosafety Res.

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Development of transgenic triticale as a platform for novel bio-industrial products is predicated on an environmental biosafety assessment that quantifies the potential risks associated with its release. Pollen-mediated gene flow to related species and conventional triticale varieties is one pathway for transgene movement. A tier 1 quantification of triticale hybridization was conducted by emasculating and hand pollinating flowers under greenhouse conditions. Approximately 2000 manual pollinations were conducted for each cross and its reciprocal between two triticale genotypes: a modern triticale cultivar (AC Alta) and primary triticale (89TT108), and common wheat, durum wheat and rye. The frequency of outcrossing, hybrid seed appearance and weight, and F 1 emergence and fertility were recorded. Outcrossing, F 1 emergence and fertility rates were high from crosses between triticale genotypes. Outcrossing in inter-specific crosses was influenced by the species, and the genotype and gender of the triticale parent. In crosses to common and durum wheat where triticale was the male parent, outcrossing was ≥ 73.0% and ≥ 69.5%, respectively, but ≤ 23.9% and ≤ 3.0% when triticale was the female parent. Overall, outcrossing with rye was lower than with common and durum wheat. F 1 hybrid emergence was greater when triticale was the female parent. With the exception of a single seed, all wheat-triticale F 1 hybrid seeds were non-viable when triticale was the male parent in the cross. Only seven durum wheat-triticale F 1 hybrids emerged from 163 seeds sown, and all were produced with triticale 89TT108 as female parent. With rye, 8 F 1 hybrids emerged from 38 seeds sown, and all were produced from crosses to AC Alta; five with AC Alta as the female parent and three as the male. Interspecific F 1 hybrids were self-sterile, with the exception of those produced in crosses between common wheat and triticale where triticale was the female parent. Tier 2 hybridization quantification will be conducted under field conditions.

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Section: Discussionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 49%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of crossability between triticale (XTriticosecaleWittmack) and common wheat, durum wheat and rye

Hills¹,

Hall²,

Messenger³

et al. 2007

Environ. Biosafety Res.

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“…Only the hybrid 'Yécora' × HTC178 reached maturity and was able to be backcrossed with tritordeum. Hybrid necrosis has been observed in hybrids of similar genome constitution such as wheat × triticale and this fact has been one of the obstacles to interchange genetic information between both species (Bizimungu et al 1998). In order to increase the chance of obtaining viable and fertile plants we used a mixture of pollen from different genotypes when backcrossing.…”

Section: Resultsmentioning

confidence: 99%

Introgression of 1Dx5+1Dy10 into Tritordeum

et al. 2003

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The uses of hexaploid tritordeum as a crop for human consumption require improvement of its bread-making quality. For this purpose chromosome 1D of bread wheat with the Glu-D1 allele encoding for high-molecular-weight glutenin subunits Dx5+Dy10 was introgressed into tritordeum. Different primary tritordeums were crossed with wheats carrying subunits Dx5+Dy10. The hybrids were backcrossed to tritordeum and seeds for the next backcross (or selfing) were selected for the presence of chromosome 1D using SDS-PAGE. Forty two chromosome plants carrying subunits Dx5+Dy10 were obtained after two backcrosses and selfing. Chromosome characterization of these plants using fluorescence in situ hybridisation (FISH) proved that either chromosome substitution 1H(ch)/1D or 1A/1D had been obtained. A homozygous plant with a translocation of the entire 1DL arm to 1H(ch)S was also obtained. The complete chromosome substitution lines have better agronomic characteristics than the lines with translocations.

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Molecular mapping of hybrid necrosis genes Ne1 and Ne2 in hexaploid wheat using microsatellite markers

et al. 2006

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Hybrid necrosis is the gradual premature death of leaves or plants in certain F1 hybrids of wheat (Triticum aestivum L.), and it is caused by the interaction of two dominant complementary genes Ne1 and Ne2 located on chromosome arms 5BL and 2BS, respectively. To date, molecular markers linked to these genes have not been identified and linkage relationships of the two genes with other important genes in wheat have not been established. We observed that the F1 hybrids from the crosses between the bread wheat variety 'Alsen' and four synthetic hexaploid wheat (SHW) lines (TA4152-19, TA4152-37, TA4152-44, and TA4152-60) developed at the International Maize and Wheat Improvement Center (CIMMYT) exhibited hybrid necrosis. This study was conducted to determine the genotypes of TA4152-60 and Alsen at the Ne1 and Ne2 loci, and to map the genes using microsatellite markers in backcross populations. Genetic analysis indicated that Alsen has the genotype ne1ne1Ne2Ne2 whereas the SHW lines have Ne1Ne1ne2ne2. The microsatellite marker Xbarc74 was linked to Ne1 at a genetic distance of 2.0 cM on chromosome arm 5BL, and Xbarc55 was 3.2 cM from Ne2 on 2BS. Comparison of the genetic maps with the chromosome deletion-based physical maps indicated that Ne1 lies in the proximal half of 5BL, whereas Ne2 is in the distal half of 2BS. Genetic linkage analysis showed that Ne1 was about 35 cM proximal to Tsn1, a locus conferring sensitivity to the host selective toxin Ptr ToxA produced by the tan spot fungus. The closely linked microsatellite markers identified in this study can be used to genotype parental lines for Ne1 and Ne2 or to eliminate the two hybrid necrosis genes using marker-assisted selection.

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Hybrid necrosis as a barrier to gene transfer in hexaploid winter wheat × triticale crosses

Cited by 15 publications

References 19 publications

Evaluation of crossability between triticale (XTriticosecaleWittmack) and common wheat, durum wheat and rye

Evaluation of crossability between triticale (XTriticosecaleWittmack) and common wheat, durum wheat and rye

Introgression of 1Dx5+1Dy10 into Tritordeum

Molecular mapping of hybrid necrosis genes Ne1 and Ne2 in hexaploid wheat using microsatellite markers

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