Hybrid Embryo Rescue in Crop Improvement

Sahijram, Leela; Rao, B. Madhusudhana

doi:10.1007/978-81-322-2283-5_18

Cited by 8 publications

(12 citation statements)

References 45 publications

(23 reference statements)

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“…It has been used in crop improvement by intraspecific/interspecific/intergeneric hybrid development, haploid/double haploid production, overcoming embryo abortion, overcoming seed dormancy, overcoming self- and cross-incompatibility, shortening the breeding cycle, propagating rare plants, etc. [ 226 , 227 , 228 ]. For example, breeding cycles were shortened by embryo rescue in rose [ 229 ], and lily [ 230 ].…”

Section: Application Of In Vitro Techniques In Ornamentalsmentioning

confidence: 99%

Tissue Culture in Ornamentals: Cultivation Factors, Propagation Techniques, and Its Application

Mumivand

Akter

et al. 2022

Plants

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Ornamentals come in a variety of shapes, sizes, and colors to suit a wide range of climates, landscapes, and gardening needs. Compared to demand, a shortage of plant materials and diversity force the search for solutions for their constant acquisition and improvement to increase their commercial value, respectively. In vitro cultures are a suitable solution to meet expectations using callus culture, somatic embryogenesis, protoplast culture, and the organogenesis of protocorm-like bodies; many of these techniques are commercially practiced. Factors such as culture media, explants, carbohydrates, plant growth regulators, and light are associated with the success of in vitro propagation. Techniques, especially embryo rescue and somatic hybridization, are widely used to improve ornamentals. The development of synthetic seed allows season-independent seed production and preservation in the long term. Despite the advantages of propagation and the improvement of ornamentals, many barriers still need to be resolved. In contrast to propagation and crop developmental studies, there is also a high scope for molecular studies, especially epigenetic changes caused by plant tissue culture of ornamentals. In this review, we have accumulated and discussed an overall update on cultivation factors, propagation techniques in ornamental plant tissue culture, in vitro plant improvement techniques, and future perspectives.

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Section: Application Of In Vitro Techniques In Ornamentalsmentioning

confidence: 99%

Tissue Culture in Ornamentals: Cultivation Factors, Propagation Techniques, and Its Application

Mumivand

Akter

et al. 2022

Plants

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“…Complete elimination of bulb barley chromatin occurs within 5-9 days after pollination. The use of embryo rescue technology (embryo rescue) provides an increase in the efficiency of the method at the next stage and the possibility of its use in selection (Sahijram, Rao, 2015). 4.…”

Section: Haploidymentioning

confidence: 99%

Haploid biotechnology as a tool for creating a selection material for sugar beets

Колесникова¹,

Donskikh²,

Berdnikov³

2022

Vestn. VOGiS

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Since the discovery of the phenomenon of haploidy, biotechnology has become an integral part in the successful creation of new varieties and hybrids of various plant species. In particular, these technologies are actively used in agriculture, which is concerned with increasing the volume and improving the quality of products. The integration of haploid production techniques together with other available biotechnological tools such as marker selection (MAS), induced mutagenesis and genetic engineering technologies can significantly accelerate crop breeding. This article shows the main stages in the development of biotechnology since 1921. Now they are successfully used to create doubled haploids to accelerate the selection process of various plants and, in particular, sugar beet, which is the most important sugar crop in regions with a temperate climate. There are several methods for obtaining forms with a single set of chromosomes. For sugar beets, the use of gynogenesis turned out to be expedient, since in this case the other methods turned out to be ineffective in the mass production of haploids. The article considers the stages of obtaining the H and DH lines of Beta vulgaris L., as well as the main stages of biotechnological production of homozygous breeding material of this culture. These stages include selecting parental forms – donor explants, sterilizing buds and introducing non-pollinated ovules in vitro, obtaining haploids, doubling their chromosome set, creating doubled haploids, determining ploidy at different stages, relocating the obtained plants to greenhouses and growing stecklings. A number of advantages that the technology of creating doubled haploids in vitro has in comparison with traditional methods of selection are described. It has been shown that the use of these approaches is relevant when obtaining new highly productive hybrids and varieties of agricultural plants; however, the methods for the production of homozygous forms in sugar beet still require additional research aimed at increasing the efficiency and reproducibility of each stage of the process.

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“…Результаты этих исследований не только подтвердили элиминацию хромосом луковичного ячменя, но и выявили критические периоды развития зародыша, сроки гибели эндосперма и необходимость дальнейшего культивирования эмбрионов на искусственной питательной среде для завершения процесса развития и прорастания. Применение технологии embryo rescue обеспечило на следующем этапе повышение эффективности метода и его дальнейшее использования в селекционном процессе (Konzak et al, 1951;Bhojwani, Dantu, 2013;Filippis, 2014;Lulsdorf et al, 2014;Sahijram, Rao, 2015). В работе Sanie с коллегами (Sanie et al, 2011) установлено, что селективная элиминации хромосом H. bulbosum опосредована утратой одного из видов гистонового белка H3(CENH3) в центромере.…”

Section: получение гаплоидов в скрещиванияхunclassified

“…Одно из проявлений постгамной несовместимости -отсутствие развития эндосперма, приводящее к голоданию зародыша и его последующей гибели. Своевременная изоляция зародыша и его культивирование на искусственной питательной среде in vitro является основополагающим принципом технологии embryo rescue («спасения» незрелых зародышей), обеспечивающей получение жизнеспособных растений в отдаленных скрещиваниях (Filippis, 2014;Lulsdorf et al, 2014;Sahijram, Rao, 2015;Gosal, Wani, 2018).…”

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Distant hybridization as a method of haploid production in cereals

Дьячук

Akinina

Хомякова

et al. 2019

Biotehnologiâ i selekciâ rastenij

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Elimination of chromosomes is a phenomenon widespread in distant hybrids. It ranges from the loss of one or two chromosomes to elimination of whole chromosome complement of one of the parents. Such elimination leads to the production of haploid plants, which then are treated with colchicine to double the chromosome number and to develop DH‑lines. Homozygosity of doubled haploids serves as a basis for their wide use in plant genetics and breeding. The use of this approach reduces the time required for obtaining homozygous lines by 5 years on the average. It leads to savings in human resources, energy and acreage. The development of the “bulbosum” method for haploid barley production had a strong influence on the chromosome engineering in cereals and its implementation in plant breeding. However, the method developed on that basis could not be used effectively for producing haploids of wheat, triticale, etc. because of Hordeum bulbosum L. pollen sensitivity to genes inhibiting wheat crossability (Kr genes). The crosses with Imperata cylindrica (L.) Raeusch. is an efficient alternative to the widely used wheat × maize and triticale × maize crosses due to abundant pollen supply within a longer time period, significantly higher frequency of embryos formation and haploid plants regeneration.

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Hybrid Embryo Rescue in Crop Improvement

Cited by 8 publications

References 45 publications

Tissue Culture in Ornamentals: Cultivation Factors, Propagation Techniques, and Its Application

Tissue Culture in Ornamentals: Cultivation Factors, Propagation Techniques, and Its Application

Haploid biotechnology as a tool for creating a selection material for sugar beets

Distant hybridization as a method of haploid production in cereals

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