Hybrid Cell Line from a Cloned Immunoglobulin-Producing Mouse Myeloma and a Nonproducing Mouse Lymphoma

Mohit, Behzad; Fan, Kang

doi:10.1126/science.171.3966.75

Cited by 55 publications

(15 citation statements)

References 11 publications

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“…This cell line was established from a lymphoma induced in a C57BL/6n mouse by 9,10-dimethyl-1,2-benzanthracene (9 (4). This underestimation of chromosome numbers was the result of errors of direct counting of chromosomes under the microscope.…”

Section: Resultsmentioning

confidence: 99%

“…A hybrid cell line was established by cell fusion between a cloned BALB/c myeloma that produced immunoglobulin IgG and free kappa chain, and C57BL/6n lymphoma that did not produce any immunoglobulins. The resulting hybrid cell population synthesized free kappa chain, but no synthesis of IgG (Y2a) heavy chain was detected (4). I now report isolation of IgG (Y2a) heavy-chain producers from these hybrid cells by a single-cell cloning technique.…”

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confidence: 99%

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Immunoglobulin G and Free Kappa-Chain Synthesis in Different Clones of a Hybrid Cell Line

Mohit

1971

Proc. Natl. Acad. Sci. U.S.A.

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ABSTRACT13 clonal cell lines were isolated from a hybrid cell population established by cell fusion between cloned BALB/c myeloma cells that were resistant to 8-azaguanine and produced immunoglobulin G and free kappa chain and Cs7BL/6n lymphoma cells that were resistant to bromodeoxyuridine and did not produce immunoglobulins. Some of the histocompatibility-2 antigens of both parental cell lines could be demonstrated on all of the hybrid clones. 11 clones synthesized only free kappa chain. Two clones synthesized IgG of BALB/c type, as well as free kappa chain. These clones had higher chromosome number than did the clones that synthesized only kappa chains.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Immunoglobulin G and Free Kappa-Chain Synthesis in Different Clones of a Hybrid Cell Line

Mohit

1971

Proc. Natl. Acad. Sci. U.S.A.

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“…1). Although significant killing was observed as early as 24 hr after addition of the steroid, the data shown represent the effect at 48 hr. The observation that CL4 cells were sensitive to dexamethasone was rather unexpected, since certain myelomas are reported to be insensitive to glucocorticoids (9).…”

Section: Characteristics Of the Parental Cell Lines And The Hybrid CLmentioning

confidence: 99%

“…The myeloma parent cell line (CL4) was an 8-azaguanineresistant clone derived from the Balb/c mouse myeloma tumor, RPC-5 (24). It synthesized IgG as well as free K chains and had a modal chromosome number of 60 (see Table 1).…”

Section: Characteristics Of the Parental Cell Lines And The Hybrid CLmentioning

confidence: 99%

Glucocorticoid Action on Hybrid Clones Derived from Cultured Myeloma and Lymphoma Cell Lines

Gehring

Mohit

Tomkins

1972

Proc. Natl. Acad. Sci. U.S.A.

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Cells of a cloned myeloma line from a Balb/c mouse contain specific cytoplasmic glucocorticoid receptors and are killed by dexamethasone. Cells of a lymphoma line (from mouse strain C57BL) also contain specific glucocorticoid receptors but are resistant to the steroid. Cells of two hybrid clones with widely differing chromosome numbers, derived by fusion between the resistant lymphoma and the sensitive myeloma, contain specific glucocorticoid receptors with similar binding properties as the parental receptors and are killed by dexainethasone. Since the lethal effect of the steroid is expressed ini the hybrid cells, failure of the parent lymphoma line to be affected by dexamethasone is probably not due to an inhibitor of the lethal reaction.

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“…8 RPC-5.4 myeloma cells were obtained from ATCC (TIB-12; Manassas, VA) 36 and used as a source for isotype-matched control antibody. Antibodies were affinity purified on protein-G Sepharose (Amersham Biosciences, Piscataway, NJ) from cell supernatants.…”

Section: Production Of Uv3mentioning

confidence: 99%

The antitumor activity of an anti‐CD54 antibody in SCID mice xenografted with human breast, prostate, non‐small cell lung, and pancreatic tumor cell lines

Brooks

Coleman

Vitetta

2008

Intl Journal of Cancer

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We have previously described the development and testing of a monoclonal anti-human CD54 antibody (UV3) in SCID mice xenografted with human multiple myeloma, lymphoma, and melanoma cell lines. In all 3 cases, UV3 was highly effective at slowing the growth of tumors and/or prolonging survival. Since CD54 (ICAM-1) is up-regulated on many different types of cancer cells, we have now investigated the anti-tumor activity of UV3 in several other CD54 1 epithelial tumors. A panel of 16 human breast, prostate, non-small cell (NSC) lung, and pancreatic tumor cell lines was examined for reactivity with UV3, and 13 were positive. A representative CD54 1 cell line from each cancer was grown subcutaneously in SCID mice. Once the tumors were established, UV3 was administered using different dose regimens. UV3 slowed the growth of all 4 tumors, although it was not curative. When UV3 or gemcitabine were administered to SCID mice xenografted with a NSC lung tumor cell line or a pancreatic tumor cell line, UV3 was as effective as the chemotherapy alone. When gemcitabine and UV3 were administered together, the best anti-tumor responses were observed. UV3 has been chimerized (cUV3) and both toxicology studies and clinical trials are planned to assess the safety and activity of cUV3 in patients with one or more of these tumors.

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Hybrid Cell Line from a Cloned Immunoglobulin-Producing Mouse Myeloma and a Nonproducing Mouse Lymphoma

Cited by 55 publications

References 11 publications

Immunoglobulin G and Free Kappa-Chain Synthesis in Different Clones of a Hybrid Cell Line

Immunoglobulin G and Free Kappa-Chain Synthesis in Different Clones of a Hybrid Cell Line

Glucocorticoid Action on Hybrid Clones Derived from Cultured Myeloma and Lymphoma Cell Lines

The antitumor activity of an anti‐CD54 antibody in SCID mice xenografted with human breast, prostate, non‐small cell lung, and pancreatic tumor cell lines

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