Hybrid 3D Printing of Synthetic and Cell‐Laden Bioinks for Shape Retaining Soft Tissue Grafts

Belleghem, Sarah Van; Torres, Leopoldo; Santoro, Marco; Mahadik, Bhushan; Wolfand, Arley; Kofinas, Peter; Fisher, John P.

doi:10.1002/adfm.201907145

Cited by 57 publications

(41 citation statements)

References 47 publications

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“…Naturally derived polymers such as alginate, fibrin, HA, collagen, and gelatin are highly printable using extrusion-based bioprinting. They offer a low donor-site morbidity and invoke a low immune response [ 88 ]. However, in vivo, they often undergo rapid degradation and/or remodeling which decreases their mechanical stability and fidelity over time.…”

Section: Bioink Requirementsmentioning

confidence: 99%

Bioprintability: Physiomechanical and Biological Requirements of Materials for 3D Bioprinting Processes

et al. 2020

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Three-dimensional (3D) bioprinting is an additive manufacturing process that utilizes various biomaterials that either contain or interact with living cells and biological systems with the goal of fabricating functional tissue or organ mimics, which will be referred to as bioinks. These bioinks are typically hydrogel-based hybrid systems with many specific features and requirements. The characterizing and fine tuning of bioink properties before, during, and after printing are therefore essential in developing reproducible and stable bioprinted constructs. To date, myriad computational methods, mechanical testing, and rheological evaluations have been used to predict, measure, and optimize bioinks properties and their printability, but none are properly standardized. There is a lack of robust universal guidelines in the field for the evaluation and quantification of bioprintability. In this review, we introduced the concept of bioprintability and discussed the significant roles of various physiomechanical and biological processes in bioprinting fidelity. Furthermore, different quantitative and qualitative methodologies used to assess bioprintability will be reviewed, with a focus on the processes related to pre, during, and post printing. Establishing fully characterized, functional bioink solutions would be a big step towards the effective clinical applications of bioprinted products.

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Section: Bioink Requirementsmentioning

confidence: 99%

Bioprintability: Physiomechanical and Biological Requirements of Materials for 3D Bioprinting Processes

et al. 2020

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“…It has been reported that 3D printing can be an ideal approach to fabricate various micro/macroscale intricate structures owing to its outstanding repeatability and reproducibility which is assisted by a computer-aided method. This emerging technology has started a new era of designing and manufacturing of tissue engineering cell-laden substitutes and biological constructs [ 25 , 26 ]. In this study, CPC scaffolds with interconnected pores (diameter 5 mm, thickness 2 mm) printed using calcium phosphate semi-solid paste (α-tricalcium phosphate and calcium-deficient hydroxyapatite) exhibited rough surface morphology which could be attributed to the microcrystalline morphology of the CPC.…”

Section: Resultsmentioning

confidence: 99%

3D Printed Calcium Phosphate Cement (CPC) Scaffolds for Anti-Cancer Drug Delivery

Woodbine

Carr

et al. 2020

Pharmaceutics

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One of the main applications of bone graft materials is filling the gap after the surgical removal of bone cancer or tumors. Insufficient healing commonly leads to non-union fracture which could lead to cancer resurgence or infection. Emerging 3D printing of on-demand bone graft biomaterials can deliver personalized solutions with minimized risk of relapse and recurrence of cancer after bone removal surgery. This research aims to explore 3D printed calcium phosphate cement (CPC) based scaffolds as novel anti-cancer drug delivery systems to treat bone cancer. For the study, various 3D printed CPC based scaffolds (diameter 5 mm) with interconnected pores were utilized. Various optimized polymeric solutions containing a model anticancer drug 5-fluorouracil (5-FU) was used to homogenously coat the CPC scaffolds. Both hydrophilic Soluplus (SOL) and polyethylene glycol (PEG) and a combination of both were used to develop stable coating solutions. The surface morphology of the coated scaffolds, observed via SEM, revealed deposition of the polymeric solution represented by a semi-smooth surface as opposed to the blank scaffolds that showed a smoother surface. An advanced surface analysis conducted via confocal microscopy showed a homogenous distribution of the drug throughout the coated scaffolds. Solid-state analysis studied by applying differential scanning calorimetry (DSC) and X-ray diffraction (XRD) revealed semi-crystalline nature of the drug whereas mechanical analysis conducted via texture analysis showed no evidence in the change of the mechanical properties of the scaffolds after polymeric solutions were applied. The FTIR analysis revealed no major intermolecular interactions between 5-FU and the polymers used for coatings except for F2 where a potential nominal interaction was evidenced corresponding to higher Soluplus content in the formulation. In vitro dissolution studies showed that almost 100% of the drug released within 2 h for all scaffolds. Moreover, in vitro cell culture using two different cell lines (Hek293T-human kidney immortalized cell line and HeLa-human bone osteosarcoma epithelial cell line) showed significant inhibition of cell growth as a function of decreased numbers of cells after 5 days. It can be claimed that the developed 5-FU coated 3D printed scaffolds can successfully be used as bone graft materials to potentially treat bone cancer or bone neoplasm and for personalized medical solutions in the form of scaffolds for regenerative medicine or tissue engineering applications.

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“…Nevertheless, the type of plastic molten wax can easily produce the desired object [20]. And the use of software that can be used for 3d printers is already present as software that is capable of producing shapes with the concept of 3 axes (x, y, z) or, in other words displaying data in 3D format [21].…”

Section: Aspects In a 3d Printermentioning

confidence: 99%

Utilization of Three Dimensional Printers as a Production Tool

Ramadhan¹,

Syarifuddin²,

Cahyaningrum³

et al. 2021

Advances in Social Science, Education and Humanities Research

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A 3D printer is a product resulting from the development of a product. As a product, a 3-dimensional printer has its value as an object with a function because with its function as a printing tool; the printer can produce an object in physical form. So that it can produce real objects as the output. By using a qualitative descriptive method, this research gets the result that all elements contained in a 3-dimensional printer can not only produce a real object that is following its initial function. In line with the method, this study aims to explain the function of a 3-dimensional printer, which can directly produce products. Other than that, 3 Dimensional printers are now present as tools that can make objects real. So that it has a positive impact on the creation of an object. 3-dimensional printers are still owned by limited circles, but indirectly it can influence potential users of these tools in the form of demands regarding knowledge in the process of making an object using a 3-dimensional printer.

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Hybrid 3D Printing of Synthetic and Cell‐Laden Bioinks for Shape Retaining Soft Tissue Grafts

Cited by 57 publications

References 47 publications

Bioprintability: Physiomechanical and Biological Requirements of Materials for 3D Bioprinting Processes

Bioprintability: Physiomechanical and Biological Requirements of Materials for 3D Bioprinting Processes

3D Printed Calcium Phosphate Cement (CPC) Scaffolds for Anti-Cancer Drug Delivery

Utilization of Three Dimensional Printers as a Production Tool

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