Hyaluronic Acid Reduces Bacterial Fouling and Promotes Fibroblasts’ Adhesion onto Chitosan 2D-Wound Dressings

Abstract

Wound healing is a dynamic process that can be seriously delayed by many factors including infectious complications. The development of dressings with intrinsic wound healing activity and/or releasing bioactive compounds may help with addressing such an issue. In this study, hyaluronic acid (HA) at different percentages (1–35%) was used to modify chitosan (CS) biological and physico-chemical properties in order to obtain 2D-matrices able to promote healing and protect from infection. HA incorporation in the CS matrix decreased film transparency and homogeneity, but improved film water uptake and surface wettability. The water vapor transmission rate (WVTR) increased up to a 5% HA content, where it reached the highest value (672 g/m2 day), and decreased for higher HA contents. At all of the tested HA concentrations, HA affected mechanical properties providing matrices more flexible than pure CS with benefit for wound care. Pure CS films permitted S. epidermidis adhesion and biofilm formation. That was not true for CS/HA matrices, where HA at concentrations equal to or greater than 5% was able to avoid S. epidermidis adhesion. Fibroblasts adhesion also took benefit from the HA presence in the film, especially at 5% content, where the best adhesion and proliferation was found.

Chitosan scaffolds with enhanced mechanical strength and elastic response by combination of freeze gelation, photo-crosslinking and freeze-drying

“…However, these soft materials often suffer from poor mechanical resistance and low stability in water environment, which may compromise their in-use performance. That's also the case of chitosan, a natural polycation deriving from the partial deacetylation of chitin, largely investigated for biomedical applications, because of its outstanding biological properties including mucoadhesivity and antimicrobial properties (Amato et al, 2018;Cuzzucoli Crucitti et al, 2018;Francolini, Donelli, Crisante, Taresco, & Piozzi, 2015;Silvestro et al, 2020;Wang et al, 2020). Additionally, more than other natural polymers, chitosan triggers a minimal foreign-body response and fibrous encapsulation (Bhattarai, Edmondson, Veiseh, Matsen, & Zhang, 2005;Jayakumar, Prabaharan, Nair, & Tamura, 2010;Kim et al, 2008).…”

Preparation and Characterization of TPP-Chitosan Crosslinked Scaffolds for Tissue Engineering

“…Among polysaccharides, chitosan (CS) is the most employed thanks to its similarity to naturally occurring glycosaminoglycan and its excellent properties like biocompatibility, biodegradability, non-toxicity, and easy processability. Recently, CS combined with nanoparticles, fibres or blended with other polymers has achieved a predominant role as a candidate for biomedical applications [9][10][11]. CS is a linear copolymer composed of D-glucosamine and N-acetyl-D-glucosamine units connected by (1)(2)(3)(4) glyosidic bonds obtained by N-deacetylation of chitin.…”

Recent Advances in Nanozymes for Bacteria-Infected Wound Therapy

“…The materials used include the antioxidants polyurethane, collagen, bacterial cellulose, CS, silica gel, HA, alginate, etc. [141][142][143][144][145][146][147][148] In recent years, studies have found that lasers and tissue-engineered alternatives can shorten the healing time of leg ulcers, pressure ulcers, diabetes, and wounds at high risk of infection. 15 Compared with traditional treatment methods, modern treatment methods have solved the problems of dosage, safety, formulation, cost, production method, batch, and efficacy to a certain extent.…”