Hyaluronic Acid Alleviates Oxidative Stress and Apoptosis in Human Tenocytes via Caspase 3 and 7

Gallorini, M.; Passeri, Cecilia; Cataldi, Amelia; Berardi, Anna C.; Osti, Leonardo

doi:10.3390/ijms23158817

Cited by 8 publications

(6 citation statements)

References 34 publications

(45 reference statements)

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“…The mixture of HA with other natural substances was also found to improve the therapeutic activity of HA [ 36 , 37 ], and recently, HA was also proposed in the treatment of airway diseases improving functional parameters in COPD patients [ 38 ]. The interrelationship between oxidative damage and inflammation is well demonstrated, and the role of HA in scavenging oxygen radicals has been recently reported [ 39 , 40 ].…”

Section: Discussionmentioning

confidence: 99%

The Anti-Inflammatory Effect of Lactose-Modified Hyaluronic Acid Molecules on Primary Bronchial Fibroblasts of Smokers

et al. 2023

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The progression of smoking-related diseases is characterized by macrophage-mediated inflammation, which is responsible for an increased expression of proinflammatory cytokines and galectins, molecules that bind specifically to β-galactoside sugars. This study aimed to assess the anti-inflammatory and antioxidant effects of a broad selection of differently lactose-modified hyaluronic acids (HA) named HYLACH®, which are able to bind proinflammatory galectins. The best HYLACH ligands for Gal-3 were selected in silico and their activities were tested in vitro on primary human bronchial fibroblasts obtained from smokers and inflamed with the conditioned medium of activated U937 monocytes. Changes in cell viability, ROS generation, proinflammatory mediators, and MMP expression, at both gene and protein levels, were analyzed. The in silico results show that HYLACH with a percentage of lactosylation of 10–40% are the best ligands for Gal-3. The in vitro study revealed that HYLACH compounds with 10, 20, and 40% lactosylation (HYLACH-1-2-3) administrated to inflamed cell cultures counteracted the oxidative damage and restored gene and protein expression for IL-1β, TNF-α, IL-6, Gal-1, Gal-3, and MMP-3 to near baseline values. The evidence that HYLACH attenuated macrophage-induced inflammation, inhibited MMP expression, and exhibited antioxidative effects provide an initial step toward the development of a therapeutic treatment suitable for smoking-related diseases.

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Section: Discussionmentioning

confidence: 99%

The Anti-Inflammatory Effect of Lactose-Modified Hyaluronic Acid Molecules on Primary Bronchial Fibroblasts of Smokers

et al. 2023

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“…Hyaluronic acid, which has been suggested as a treatment for tendon disorders and has strong antioxidant potential, protects against oxidative-stress-induced apoptosis in tenocytes by reducing the activation of Caspase 3 and Caspase 7 (stimulators of apoptosis). Hyaluronic acid treatment also significantly improves cell viability [107]. Similarly, in a study by Gallorini et al, hyaluronic acid administration provided protection against oxidative-stress-related cytotoxicity and reduced Nrf2 expression [108].…”

Section: Oxidative Stress Inhibition Promotes Cell Viability In Vitromentioning

confidence: 75%

Hypoxia-Inducible Factor and Oxidative Stress in Tendon Degeneration: A Molecular Perspective

Shahid,

Morya,

et al. 2024

Antioxidants

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Tendinopathy is a debilitating condition marked by degenerative changes in the tendons. Its complex pathophysiology involves intrinsic, extrinsic, and physiological factors. While its intrinsic and extrinsic factors have been extensively studied, the role of physiological factors, such as hypoxia and oxidative stress, remains largely unexplored. This review article delves into the contribution of hypoxia-associated genes and oxidative-stress-related factors to tendon degeneration, offering insights into potential therapeutic strategies. The unique aspect of this study lies in its pathway-based evidence, which sheds light on how these factors can be targeted to enhance overall tendon health.

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“…In SAOS-2 cells, all changes were observed following HA treatment, particularly at a concentration of 1000 µg/mL. Gallorini and his colleagues highlighted the potential pro-apoptotic effect of HA in tendon-derived cells [9]. The use of HA in a targeted transport system of doxorubicin led to similar morphological changes in breast cancer cells -MCF-7 [34].…”

Section: Resultsmentioning

confidence: 95%

In Vitro Assessment of Two Types of Hyaluronic Acid’s Antitumor Potential in Osteosarcoma Cells

ILIA

2023

FARMACIA

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Even after the remarkable progress of the last decade, osteosarcoma remains a serious problem on a global scale, mainly affecting teenagers and elderly people. Hyaluronic acid is a ubiquitous polysaccharide in vertebrates and plays important physiological roles in the body, including the regulation of cell proliferation and inflammation. There are a variety of medical applications for hyaluronic acid; recent research has highlighted its role in the mineralization and growth of bones. Following these theoretical considerations, the present study sought to evaluate the antitumor potential of two types of hyaluronic acid in osteosarcoma cells. In order to achieve this goal, the viability and morphology of the cells, the effect on the structure of the nuclei, and the gene expression of some pro-and anti-apoptotic genes were evaluated. The results of the study indicated that hyaluronic acid causes a dose-dependent decrease in cell viability and induces changes in morphology and nuclei characteristic of cell apoptosis. Additionally, the highest concentrations tested resulted in an increase in pro-apoptotic genes (Bax and Bad) and a decrease in anti-apoptotic genes (Bcl-2). In conclusion, hyaluronic acid may represent a possible effective candidate for the treatment of osteosarcoma, but further studies are required in order to clarify the molecular mechanisms involved. RezumatÎn ciuda progreselor remarcabile din ultima perioadă, osteosarcomul reprezintă în continuare o problemă ingrijorătoare la nivel global, afectând în principal adolescenții și persoanele de peste 60 de ani. Acidul hialuronic este o polizaharidă omniprezentă la vertebrate și care joacă roluri fiziologice importante în organism, precum reglarea proliferării celulare și inflamației celulare. În practica medicală, acidul hialuronic prezintă o gamă largă de aplicabilități medicale, recent fiind evidențiat rolul benefic în mineralizarea și creșterea osului. Pornind de la aceste considerente teoretice, principalul obiectiv al studiului prezent a fost evaluarea potențialului antitumoral al două tipuri de acid hialuronic, la nivelul celulelor de osteosarcom. În acest scop au fost evaluate viabilitatea și morfologia celulară, impactul la nivelul structurii nucleilor precum și influența asupra unor gene pro-și anti-apoptotice. Rezultatele studiului au indicat faptul că acidul hialuronic determina o scadere dozodependentă a viabilității celulare și induce modificări la nivelul morfologiei și nucleilor caracteristice apoptozei celulare. În plus, concentrația cea mai mare testată, a determinat o creștere semnificativă a genelor pro-apoptotie (Bax si Bad) și o scădere a genei anti-apoptotice (Bcl-2). În concluzie, acidul hialuronic poate reprezenta un posibil candidat eficient în terapia antitumorala a osteosarcomului, însă sunt necesare studii suplimentare pentru elucidarea mecanismelor biologice.

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Hyaluronic Acid Alleviates Oxidative Stress and Apoptosis in Human Tenocytes via Caspase 3 and 7

Cited by 8 publications

References 34 publications

The Anti-Inflammatory Effect of Lactose-Modified Hyaluronic Acid Molecules on Primary Bronchial Fibroblasts of Smokers

The Anti-Inflammatory Effect of Lactose-Modified Hyaluronic Acid Molecules on Primary Bronchial Fibroblasts of Smokers

Hypoxia-Inducible Factor and Oxidative Stress in Tendon Degeneration: A Molecular Perspective

In Vitro Assessment of Two Types of Hyaluronic Acid’s Antitumor Potential in Osteosarcoma Cells

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