Hyaline bodies in subepidermal papillae. Immunohistochemical studies in several dermatoses

Ueki, H.

doi:10.1001/archderm.100.5.610

Cited by 35 publications

(21 citation statements)

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“…DIF patterns considered typical of LP, as previously described [1, 2, 5, 11,14,15,16,17], are globular deposits of immunoglobulins, principally IgM, and deposits of C3 and fibrinogen localized in the papillary dermis adjacent to the dermoepidermal junction. In some cases, globular deposits may be present in the external root sheath of the hair follicles [20].…”

Section: Methodsmentioning

confidence: 88%

“…Additionally, a diffuse band of fibrin stains along the dermoepidermal junction and around follicles [15, 16]. Typically the globular deposits in LP are numerous, tend to cluster and usually contain various immunoglobulins and C3 [14, 16, 17]. …”

Section: Methodsmentioning

confidence: 99%

“…The most characteristic DIF findings in LP consist of grouped globular deposits of IgM (cytoid bodies), adjacent to the follicular epithelium or at the dermoepidermal junction (fig. 1), and heavy deposits of fibrin at the dermal-epidermal junction [1, 2, 5, 11,14,15,16,17]. In LE, DIF studies most commonly demonstrate granular deposits of immunoglobulin (fig.…”

Section: Introductionmentioning

confidence: 99%

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Value of Direct Immunofluorescence for Differential Diagnosis of Cicatricial Alopecia

Trachsler

Trüeb²

2005

Dermatology

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Background: There are diverse causes of cicatricial alopecia characterized by lack of follicular ostia and irreversible loss of hair. While clinical differentiation between the causes may be difficult, particularly with regard to lichen planus (LP), lupus erythematosus (LE) and pseudopelade of Brocq (PB), it has been suggested that both histopathologic examination and direct immunofluorescence studies (DIF) are necessary for an accurate diagnosis. Objective: The aim of this study was to evaluate the diagnostic value of DIF studies in addition to histopathology in patients with cicatricial alopecia as a clinical feature. Methods: 136 scalp biopsy specimens received for histopathology and DIF during a 5-year period were reviewed. Results: Definitive diagnosis was achieved by careful evaluation of scalp biopsies. The most prevalent diagnoses in order of frequency were LP (26%), LE (21%) and folliculitis decalvans (20%). PB was diagnosed in 10%. In most cases, the diagnosis could be made on the basis of histopathology and independently of DIF. Characteristic DIF patterns showed high specificity, but low sensitivity for LP, and high specificity and sensitivity for LE. The DIF pattern in PB showed no difference to LP. Conclusions: Histopathology permits diagnosis in the majority of cicatricial alopecias. DIF is of value in histopathologically inconclusive cases, particularly when LE is in question.

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Section: Methodsmentioning

confidence: 88%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Value of Direct Immunofluorescence for Differential Diagnosis of Cicatricial Alopecia

Trachsler

Trüeb²

2005

Dermatology

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“…Anti-DNP was labelled with fluorescein isothiocya nate (FITC) and purified by column chromatography (Sephadex G-25 and DEAE cellulose) by a procedure described previously [19]. The labeled antibody had the following characteristics: FITC 30 pg/ml, total protein 7.5 mg/ml, fluorescein protein ratio 1:64 (molar) or 4.0 x 10~3 (w/w).…”

Section: Immunofluorescent Methodsmentioning

confidence: 99%

Distribution of 2,4-Dinitrophenyl Groups on the Epidermal Langerhans Cells of Guinea Pigs following Skin Painting with 2,4-Dinitrochlorobenzene

Oka¹,

Nakagawa²,

Oota³

et al. 1986

Dermatology

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It has been postulated that epidermal Langerhans cells (LC) are essential for induction and elicitation of contact sensitivity (CS) to simple chemical allergens. Although the precise role of LC in CS has not been determined, recent reports have focused on a function for LC in the presentation of antigen to T lymphocytes. To investigate this possibility, the distribution of allergen on the epidermal LC of inbred strain 13 guinea pigs was observed by the immunofluorescent method using antibodies against la antigen and hapten. 2,4-Dinitrophenyl groups were found to be localized on/in Ia-positive epidermal LC of the animals following skin painting with 2,4-dinitrochlorobenzene. This finding is discussed in relation to the role of LC as antigen presenting cells in CS.

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“…Anti-DNP antibody was labeled with FIFC and purified by column chromatography (Sephadex G-2S and DEAE-cellulose) by a procedure described previously (8). The labeled antibody had the following characteristics: FITC 30 flg/ml, total protein 7.S mg/ml, fluorescein protein ratio 1.64 molar or 4.0 x 10-3 weight.…”

Section: If Methodsmentioning

confidence: 99%

The Distribution of 2,4‐Dinitrophenyl Groups on Thy‐1 Positive Cells in the Epidermis of Mouse Following Skin Painting with 2,4‐Dinitrochlorobenzene

Bang

Nakagawa

Oka

et al. 1988

The Journal of Dermatology

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The distribution of 2,4-dinitrophenyl (DNP) groups on Thy-l positive epidermal cells in vivo after 2,4-dinitrochlorobenzene (DNCB) was painted on the skin surface of C3H/He mice was examined. Epidermal cell suspensions (EC) derived from trypsin-separated mouse ear skin painted with DNCBwere double-stained for DNP groups and Thy-l protein using FITC-anti-DNP antibody, biotinylated anti-mouse Thy-l,2 monoclonal antibody, and phyco-erythrin conjugated avidin. There were on the average 1.7% Thy-I" ECs; DNP groups were detectable on 77.8% of them when the specimen was treated with anti-DNP followed by anti-Thy-l,2 incubation. The incidence of Thy-LZ" Ee with DNP groups decreased when cells were treated with these antibodies in the reverse order. The significance of these findings is discussed in relationship to the mode ofDNCB Thy-I+ EC binding.

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Hyaline bodies in subepidermal papillae. Immunohistochemical studies in several dermatoses

Cited by 35 publications

References 0 publications

Value of Direct Immunofluorescence for Differential Diagnosis of Cicatricial Alopecia

Value of Direct Immunofluorescence for Differential Diagnosis of Cicatricial Alopecia

Distribution of 2,4-Dinitrophenyl Groups on the Epidermal Langerhans Cells of Guinea Pigs following Skin Painting with 2,4-Dinitrochlorobenzene

The Distribution of 2,4‐Dinitrophenyl Groups on Thy‐1 Positive Cells in the Epidermis of Mouse Following Skin Painting with 2,4‐Dinitrochlorobenzene

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