Humoral intestinal immunity against Encephalitozoon cuniculi (Microsporidia) infection in mice

Sak, Bohumil; Ditrich, Oleg

doi:10.14411/fp.2005.020

Cited by 12 publications

(11 citation statements)

References 15 publications

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“…The central role of cell-mediated immunity, IFNγ and IL-12 in the defence against microsporidial infection has been demonstrated in adoptive transfer experiments using SCID or athymic mice. [11][12][13][14] Furthermore, the significance of individual T-cell subsets in immunity against E. cuniculi has been shown using gene knock-out mice, highlighting the role of CD8 cytotoxic T lymphocytes. [15][16][17] However, all of the above-mentioned studies were focused only on extending the hosts' survival time regardless of the possible persistence of microsporidia and the development of latent infection.…”

Section: Most Of What Is Known About Microsporidia Is Based Onmentioning

confidence: 99%

“…In subsequent studies, host immunity against microsporidia has been examined intensively and the potential protective role of both humoral and cellular immunity was established. The central role of cell‐mediated immunity, IFN‐γ and IL‐12 in the defence against microsporidial infection has been demonstrated in adoptive transfer experiments using SCID or athymic mice . Furthermore, the significance of individual T‐cell subsets in immunity against E. cuniculi has been shown using gene knock‐out mice, highlighting the role of CD8 cytotoxic T lymphocytes …”

Section: Introductionmentioning

confidence: 99%

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Limited effect of adaptive immune response to control encephalitozoonosis

Sak

Kotková

Hlásková

et al. 2017

Parasite Immunology

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This study revises our understanding of the effectiveness of cell-mediated adaptive immunity and treatment against microsporidia using molecular detection and quantification of microsporidia in immunocompetent C57Bl/6 and immunodeficient CD4 and CD8 mice for the first time. We demonstrate an intense dissemination of microsporidia into most organs within the first weeks post-infection in all strains of mice, followed by a chronic infection characterized by microsporidia persistence in CD4 and C57Bl/6 mice and a lethal outcome for CD8 mice. Albendazole application reduces microsporidia burden in C57Bl/6 and CD4 mice, whereas CD8 mice experience only a temporary effect of the treatment. Surprisingly, treated CD8 mice survived the entire experimental duration despite enormous microsporidia burden. On the basis of our results, we conclude that microsporidia survive despite the presence of immune mechanisms and treatments that are currently considered to be effective and therefore that CD8 T lymphocytes represent a major, but not sole effector mechanism controlling microsporidiosis. Furthermore, the survival of mice does not correspond to spore burden, which provides new insight into latent microsporidiosis from an epidemiological point of view.

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Section: Most Of What Is Known About Microsporidia Is Based Onmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Limited effect of adaptive immune response to control encephalitozoonosis

Sak

Kotková

Hlásková

et al. 2017

Parasite Immunology

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“…As described previously in mice, humoral immunity is part of complex defence mechanisms against microsporidiosis. The main barrier against multiplication of microsporidia after oral infection is probably IELs, and specific antibodies produced in the intestine are able to enhance its effect [47]. In E. cuniculi infected rabbits, production of specific antibodies has been well documented.…”

Section: Cd4+ Cells (Si) Cd8+ Cells (Si) Pt+ Cells (Si) Igm+ Cells (Si)mentioning

confidence: 99%

Characterization of humoral and cell-mediated immunity in rabbits orally infected with Encephalitozoon cuniculi

Jeklová¹,

Levá²,

Matiašovic³

et al. 2020

Vet Res

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Encephalitozoonosis is a common infectious disease widely spread among rabbits. Encephalitozoon cuniculi, is considered as a zoonotic and emerging pathogen capable of infecting both immunocompetent and immunocompromised hosts. The aim of the study was to describe in detail the spread of the E. cuniculi in a rabbit organism after experimental infection and the host humoral and cellular immune response including cytokine production. For that purpose, healthy immunocompetent rabbits were infected orally in order to simulate the natural route of infection and euthanised at 2, 4, 6 and 8-weeks post-infection. Dissemination of E. cuniculi in the body of the rabbit was more rapid than previously reported. As early as 2 weeks post-infection, E. cuniculi was detected using immunohistochemistry not only in the intestine, mesenteric lymph nodes, spleen, liver, kidneys, lungs and heart, but also in nervous tissues, especially in medulla oblongata, cerebellum, and leptomeninges. Based on flow cytometry, no conspicuous changes in lymphocyte subpopulations were detected in the examined lymphoid organs of infected rabbits. Cell-mediated immunity was characterized by ability of both CD4 + and CD8 + T cells to proliferate after stimulation with specific antigens. Th1 polarization of immune response with a predominance of IFN-γ expression was detected in spleen, mesenteric lymph nodes and Peyer's patches. The increased expression of IL-4 and IL-10 mRNA in mixed samples from the small intestine is indicative of balanced control of IFN-γ, which prevents tissue damage. On the other hand, it can enable E. cuniculi to survive and persist in the host organism in a balanced host-parasite relationship. The Th17 immunity lineage seems to play only a minor role in E. cuniculi infection in rabbits.

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“…aged 5-8 weeks were orally inoculated with the washed spores in a dose of 10 5 spores per mouse (Sak and Ditrich 2005). The mice were grouped as follows:…”

Section: Comparison Of Zinc Pva and Potassium Dichromate Preservationmentioning

confidence: 99%

Zinc PVA versus potassium dichromate for preservation of microsporidian spores of human origin

et al. 2012

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Humoral intestinal immunity against Encephalitozoon cuniculi (Microsporidia) infection in mice

Cited by 12 publications

References 15 publications

Limited effect of adaptive immune response to control encephalitozoonosis

Limited effect of adaptive immune response to control encephalitozoonosis

Characterization of humoral and cell-mediated immunity in rabbits orally infected with Encephalitozoon cuniculi

Zinc PVA versus potassium dichromate for preservation of microsporidian spores of human origin

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