Humidity gradients in the air spaces of leaves

Wong, Sherman; Canny, M. J.; Holloway‐Phillips, Meisha; Stuart‐Williams, Hilary; Cernusak, Lucas A.; Márquez, Diego A.; Farquhar, Graham D.

doi:10.1038/s41477-022-01202-1

Cited by 46 publications

(102 citation statements)

References 34 publications

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“…These two oddities are likely to be caused by unsaturation or patchiness and the principles presented by Wong et al . (2022) may help to identify when either of them occurs.…”

Section: Discussionmentioning

confidence: 99%

“…We followed the Wong et al . 's (2022) approach to estimating R ias , which is to keep a constant CO 2 concentration in the upper cuvette at about atmospheric [CO 2 ] (400 μmol mol −1 ) and reduce the CO 2 concentration in the lower cuvette until the assimilation of the lower surface reaches zero. Then, under these conditions, abaxial c i equals c w and Eqn 18 can be used to estimate R ias , having chosen m (rate of change in photosynthetic uptake with x (d u /d x )).…”

Section: Methodsmentioning

confidence: 99%

“…Experiments were carried out on a gas exchange analysis system built by Wong et al (1978Wong et al ( , 1985aWong et al ( , 2022, which employs two LI-6251 gas analyzers (Li-Cor, Lincoln, NE, USA) to measure the CO 2 concentration of the upper and lower cuvettes independently. Additionally, two LI-6800 gas exchange analyzers (Li- Cor) were used simultaneously to evaluate the gas exchange from the adaxial and abaxial surfaces of the leaf using the setup presented by Márquez et al (2021a).…”

Section: Measurementsmentioning

confidence: 99%

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Assessing the CO₂ concentration at the surface of photosynthetic mesophyll cells

et al. 2023

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We present a robust estimation of the CO 2 concentration at the surface of photosynthetic mesophyll cells (c w ), applicable under reasonable assumptions of assimilation distribution within the leaf. We used Capsicum annuum, Helianthus annuus and Gossypium hirsutumas model plants for our experiments.We introduce calculations to estimate c w using independent adaxial and abaxial gas exchange measurements, and accounting for the mesophyll airspace resistances.The c w was lower than adaxial and abaxial estimated intercellular CO 2 concentrations (c i ). Differences between c w and the c i of each surface were usually larger than 10 μmol mol −1 . Differences between adaxial and abaxial c i ranged from a few μmol mol −1 to almost 50 μmol mol −1 , where the largest differences were found at high air saturation deficits (ASD). Differences between adaxial and abaxial c i and the c i estimated by mixing both fluxes ranged from −30 to +20 μmol mol −1 , where the largest differences were found under high ASD or high ambient CO 2 concentrations.Accounting for c w improves the information that can be extracted from gas exchange experiments, allowing a more detailed description of the CO 2 and water vapor gradients within the leaf.

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“…These two oddities are likely to be caused by unsaturation or patchiness and the principles presented by Wong et al . (2022) may help to identify when either of them occurs.…”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Measurementsmentioning

confidence: 99%

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Assessing the CO₂ concentration at the surface of photosynthetic mesophyll cells

et al. 2023

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“…We assumed saturated leaf internal water vapor, but leaf internal vapor pressure is thought to be unsaturated for drought-stressed plants (Vesala et al, 2017;Cernusak et al, 2018;Wong et al, 2022). Under the assumption of unsaturated leaf internal vapor, smaller leaf internal vapor pressure can be expected by adding a relative-humidity parameter lower than unity in Eq.…”

Section: Effect Of Nrw Inputs On the Air-leaf Water Exchange And Leaf...mentioning

confidence: 99%

The role of radiative cooling and leaf wetting in air–leaf water exchange during dew and radiation fog events in a temperate grassland

Riedl

Eugster

et al. 2023

Agricultural and Forest Meteorology

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“…The area of mesophyll cell wall exposed to intercellular air space ( S mes ) and the relative proportions of air, cell, and cell wall within the mesophyll can determine its resistance to CO 2 diffusion (Evans, 2021), which limits photosynthesis (Flexas et al ., 2012). In a similar way, mesophyll structure influences the rate of water loss during transpiration, as CO 2 and water travel in opposite directions along a common pathway through the mesophyll (Wong et al ., 2022). Light movement through the leaf is also affected by the shape of mesophyll cells, with elongated palisade cells facilitating the penetration of light deeper into the leaf, and the more irregularly shaped spongy mesophyll cells helping to scatter light and maximise absorption (Vogelmann and Evans, 2002; Johnson et al ., 2005).…”

Section: Introductionmentioning

confidence: 99%

Stomatal density affects rice mesophyll cell size and shape and modulates a conserved pattern of cells through the leaf

Sloan

Im-Chai

Ngai

et al. 2022

Preprint

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The structure of the mesophyll influences how light, CO2 and water travels inside a leaf, affecting the rates of both photosynthesis and transpiration. Recent studies in wheat and Arabidopsis have shown that the structure of the mesophyll is influenced by the density and activity of stomata, consistent with the hypothesis that gas flow via stomata can modulate internal cell growth and separation to co-ordinate leaf structure and function. To investigate whether this also occurs in rice, a staple food crop for a large fraction of the world's population, we examined mesophyll structure in rice mutants with altered stomatal density. Our data show that stomatal function modulates mesophyll structure in rice. Variation in the degree of mesophyll lobing made a major contribution to altered mesophyll structure, suggesting that modified leaf gas flux through stomata influences an aspect of cell shape directly linked to gas exchange capacity in rice. In addition, our analysis revealed a previously unreported underlying pattern in cell size, shape and axiality across layers of the rice mesophyll, which further investigation revealed is present in a range of rice species and cultivars. The potential origin and significance of this mesophyll patterning are discussed.

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Humidity gradients in the air spaces of leaves

Cited by 46 publications

References 34 publications

Assessing the CO₂ concentration at the surface of photosynthetic mesophyll cells

Assessing the CO₂ concentration at the surface of photosynthetic mesophyll cells

The role of radiative cooling and leaf wetting in air–leaf water exchange during dew and radiation fog events in a temperate grassland

Stomatal density affects rice mesophyll cell size and shape and modulates a conserved pattern of cells through the leaf

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Humidity gradients in the air spaces of leaves

Cited by 46 publications

References 34 publications

Assessing the CO2 concentration at the surface of photosynthetic mesophyll cells

Assessing the CO2 concentration at the surface of photosynthetic mesophyll cells

The role of radiative cooling and leaf wetting in air–leaf water exchange during dew and radiation fog events in a temperate grassland

Stomatal density affects rice mesophyll cell size and shape and modulates a conserved pattern of cells through the leaf

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Assessing the CO₂ concentration at the surface of photosynthetic mesophyll cells

Assessing the CO₂ concentration at the surface of photosynthetic mesophyll cells