2011
DOI: 10.1074/jbc.m110.210971
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Abstract: Human vitamin K 2,3-epoxide reductase complex subunit 1-like 1 (VKORC1L1), expressed in HEK 293T cells and localized exclusively to membranes of the endoplasmic reticulum, was found to support both vitamin K 2,3-epoxide reductase (VKOR) and vitamin K reductase enzymatic activities. Michaelis-Menten kinetic parameters for dithiothreitol-driven VKOR activity were:

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Cited by 85 publications
(131 citation statements)
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References 48 publications
(44 reference statements)
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“…As shown previously, VKORC1L1 proteins were able to reduce vit KϾO in vit KH2 (24). The reaction rates of recombinant human and rat VKORC1L1 toward vit K 1 ϾO followed the Michaelis-Menten model, allowing the determination of kinetic parameters (Table 1).…”
Section: Vkorc1 and Vkorc1l1 Mrna Distribution In Rat Tissues-mentioning
confidence: 98%
See 1 more Smart Citation
“…As shown previously, VKORC1L1 proteins were able to reduce vit KϾO in vit KH2 (24). The reaction rates of recombinant human and rat VKORC1L1 toward vit K 1 ϾO followed the Michaelis-Menten model, allowing the determination of kinetic parameters (Table 1).…”
Section: Vkorc1 and Vkorc1l1 Mrna Distribution In Rat Tissues-mentioning
confidence: 98%
“…Nevertheless, vertebrate genomes include two paralogous enzymes, VKORC1 and VKORC1-like 1 (VKORC1L1), likely resulting from a gene duplication of an early common VKOR ancestor. In 2011, Westhofen et al demonstrated that the VKORC1L1 gene encodes a protein able to reduce vit KϾO to vit K when VKORC1L1 is expressed in HEK293T cells (24). Nevertheless, this VKOR activity was described to present a low enzymatic efficiency.…”
mentioning
confidence: 99%
“…Consequently, almost all previous studies have assayed only the first reaction, i.e. KO to K (14,15,16,18). Previously, the antioxidant ␤-hydroxytoluene was used to try to stabilize KH 2 (20); however, tests with multiple antioxidants, including ␤-hydroxytoluene, were ineffective in blocking KH 2 oxidation (e.g.…”
Section: Vkorc1 Reduces Ko To Kh 2 To Drivementioning
confidence: 99%
“…1), and VKORC1 can only provide two electrons for a single reaction. Although it is well established that VKORC1 is responsible for KO reduction to the vitamin K quinone (K) intermediate, it has recently been proposed that an enzyme other than VKORC1 performs the reduction of K to KH 2 (17,18). A non-VKORC1 vitamin K quinone reductase exists, as bleeding defects in patients undergoing warfarin therapy can be corrected by the administration of K. Treatment involves large amounts of vitamin K, and in vitro studies suggest a high K m for the quinone reductase (19), which has not yet been identified.…”
Section: The Vitamin K Oxidoreductase (Vkorc1) Recycles Vitamin K To mentioning
confidence: 99%
“…Thus, decreased MKH availability in HCC cells is a possible causative mechanism of DCP production Vitamin K2 -Vital for Health and Wellbeingin HCC. MKH is mainly generated by a vitamin K 2, 3-epoxide reductase complex subunit 1-like-1 (VKORC1L1) in mammalian cells [38]. Key reasons for further development are that MK-4 delivery to HCC cells is poor and reductive activation of MK-4 is low in HCC cells.…”
Section: Delivery Of Menahydroquinone-4 the Active Form Of Mk-4mentioning
confidence: 99%