Human umbilical cord mesenchymal stem cell conditioned medium attenuates renal fibrosis by reducing inflammation and epithelial-to-mesenchymal transition via the TLR4/NF-κB signaling pathway in vivo and in vitro

Liu, Bo; Ding, Fengxia; Hu, Dong; Zhou, Yufeng; Chen, Long; Shen, Lianju; Zhang, Yuanyuan; Zhang, Deying; Wei, Guanghui

doi:10.1186/s13287-017-0760-6

Cited by 91 publications

(75 citation statements)

References 31 publications

(38 reference statements)

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“…As acquired immune cells, B cells secrete cytokines such as transforming growth factor-β1 (TGF-β1), tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1), and interleukin-6 (IL-6), which promotes brosis progression. A large number of studies have shown that cytokines were involved in an in ammatory response and the brosis process of kidney, liver, and lung tissues [30,31]. In recent years, there have been reports on the involvement of B cells in the myocardial brosis process of cardiovascular diseases, but the mechanism of B cells and their cytokines in the myocardial infarction process after AMI is still unclear.…”

Section: Discussionmentioning

confidence: 99%

Are activated B cells involved in the process of myocardial fibrosis after acute myocardial infarction? -An in vivo experiment

Luo

Yan

et al. 2020

Preprint

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Background: Inflammatory cells infiltrate into the ischemic and hypoxic myocardial tissue after myocardial infarction. B cells gather at the site of myocardial injury and secrete cytokines to regulate immune inflammation and fiber repair processes.Methods: The animal experiment intended to use ligation of the left anterior descending branch of C57BL/6 mice to establish a mice AMI model to observe the changes of activated B cells and cytokines at different time points. 88 12-week-old C57BL/6 male mice were divided into the Sham group (24 mice) and acute myocardial infarction (AMI ) group (64 mice) randomly. Besides, C57BL/6 Bmi-1 knock out (BKO) mice and C57BL/6 wild-type (WT) mice were used to establish AMI models to observe the expression levels of cardiomyocyte factors including TNF-α and IL-1β, IL-6, TGF-β1, COL1-A1, COL3-A1, TIMP-1, and MMP9. Moreover, pathological and collagen changes in the myocardium were analyzed. One-way ANOVA analysis of variance was used for comparison between multiple groups, and the LSD method was used for pairwise comparison between groups. P < 0.05 indicated statistical differences.Results: AMI model of C57BL/6 mice was established successfully. The ratio of activated B cells and expression of TNF-α, IL-1β, IL-6, TGF-β1, and BAFF in 5 days subgroup was highest in the myocardium, spleen, and peripheral blood with the most obvious myocardial inflammatory cells infiltration. mRNA expression levels of TNF-α, IL-1β, IL-6, TGF-β1 in 5 days subgroup of the BKO group were decreased compared with the WT group (P < 0.05). Among 2 weeks subgroups of Sham, WT, and BKO groups, the LVEDd and LVESd in the BKO and WT groups were less than those in the Sham group (P < 0.05). The LVEDd and LVESd of the BKO group were less than the WT group,EF was higher than the WT group (P < 0.05).Conclusion: Activated B cells participated in the sustained state of myocardial inflammation and immune system activation after AMI via promoting the secretion of cytokines, and may affect the metabolism of myocardial collagen. Moreover, B cells could damage the heart structure and left ventricular ejection function by promoting the expression of myocardial collagen Type I and type III.

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Section: Discussionmentioning

confidence: 99%

Are activated B cells involved in the process of myocardial fibrosis after acute myocardial infarction? -An in vivo experiment

Luo

Yan

et al. 2020

Preprint

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“…The cell-free CM derived from MSCs is enriched in various soluble factors, including growth factors, immunomodulatory molecules, cytokines, and chemokines [40,48,49]. MSC-derived CM has shown to have beneficial effects both in vivo and in vitro [50][51][52][53]. In addition, preconditioning strategies such as the environmental variations and stimulatory conditions of MSCs can change the composition of CM active factors and influence the CM's therapeutic effect.…”

Section: Msc-derived CMmentioning

confidence: 99%

Mesenchymal stem cell-derived secretomes for therapeutic potential of premature infant diseases

et al. 2020

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Preterm birth is a complex syndrome and remains a substantial public health problem globally. Its common complications include periventricular leukomalacia (PVL), bronchopulmonary dysplasia (BPD), necrotizing enterocolitis (NEC) and retinopathy of prematurity (ROP). Despite great advances in the comprehension of the pathogenesis and improvements in neonatal intensive care and associated medicine, preterm birth-related diseases remain essentially without adequate treatment and can lead to high morbidity and mortality. The therapeutic potential of mesenchymal stem/stromal cells (MSCs) appears promising as evidenced by their efficacy in preclinical models of pathologies relevant to premature infant complications. MSC-based therapeutic efficacy is closely associated with MSC secretomes and a subsequent paracrine action response to tissue injuries, which are complex and abundant in response to the local microenvironment. In the current review, we summarize the paracrine mechanisms of MSC secretomes underlying diverse preterm birth-related diseases, including PVL, BPD, NEC and ROP, are summarized, and focus is placed on MSC-conditioned media (CM) and MSC-derived extracellular vesicles (EVs) as key mediators of modulatory action, thereby providing new insights for future therapies in newborn medicine.lung function and help to remove the ventilator as soon as possible [12]. However, many studies have reported that glucocorticoid can increase the risk of adverse reactions, such as those by the nervous system, in the early or late stages, and its dose and course of treatment are not certain [13,14]. In terms of surgical treatment, preterm infants with surgical NEC are at risk of significant growth reduction and neurodevelopmental impairment (NDI), including cerebral palsy, deafness and blindness [15,16]. These sequelae and complications of prematurity-related diseases affect not only the neonatal period, but also infancy and childhood, and even adolescence and adulthood [17][18][19]. Currently, although various treatment methods have been found to achieve certain curative effects, there still exist several side effects and disputes above. Thus, it is necessary to explore and develop new therapies for the treatment and prevention of premature infant diseases.Accumulating studies have established the importance and feasibility of stem cell-based therapies to ameliorate many degenerative and inflammatory diseases. Among these stem/progenitor cell types, mesenchymal stem/stromal cells (MSCs) have received more extensive attention because they are easily extracted, exert anti-inflammatory effects, have low immunogenicity and can renew themselves. The therapeutic potential of MSCs has been demonstrated in many diseases such as cancer [20,21] and cardiovascular [22,23] and lung [24][25][26] diseases. MSCs also represent a promising and growing approach to the targeting of various pathological processes and cellular impairments underlying the major abnormalities in premature infant and preterm-associated neonatal diseases [27][28]...

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“…MSCs can differentiate into several types of cells including endothelial cells, myocytes, keratinocytes, and fibroblasts . Moreover, stem cells produce several anti‐inflammatory and antifibrotic mediators, and secrete factors that create a regenerative microenvironment . Cord blood stem cells embedded in a scaffold secrete for example MMP‐9, mediating collagen degradation in uterine scars, which improves the regeneration of the endometrium, myometrium and blood vessels .…”

Section: Umbilical Cord: From Waste Materials To Source Of Therapeuticmentioning

confidence: 99%

“…[127][128][129] Moreover, stem cells produce several anti-inflammatory and antifibrotic mediators, and secrete factors that create a regenerative microenvironment. [130][131][132][133] Cord blood stem cells embedded in a scaffold secrete for example MMP-9, mediating collagen degradation in uterine scars, which improves the regeneration of the endometrium, myometrium and blood vessels. 134 Administered cord blood stem cells also promote the recruitment of endogenous stem cells.…”

Section: Umbilical Cord: From Waste Materials To Source Of Therapeutmentioning

confidence: 99%

Tissue engineering strategies combining molecular targets against inflammation and fibrosis, and umbilical cord blood stem cells to improve hampered muscle and skin regeneration following cleft repair

Schreurs

Suttorp

Mutsaers

et al. 2019

Medicinal Research Reviews

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Cleft lip with or without cleft palate is a congenital deformity that occurs in about 1 of 700 newborns, affecting the dentition, bone, skin, muscles and mucosa in the orofacial region. A cleft can give rise to problems with maxillofacial growth, dental development, speech, and eating, and can also cause hearing impairment. Surgical repair of the lip may lead to impaired regeneration of muscle and skin, fibrosis, and scar formation. This may result in hampered facial growth and dental development affecting oral function and lip and nose esthetics. Therefore, secondary surgery to correct the scar is often indicated. We will discuss the molecular and cellular pathways involved in facial and lip myogenesis, muscle anatomy in the normal and cleft lip, and complications following surgery. The aim of this review is to outline a novel molecular and cellular strategy to improve musculature and skin regeneration and to reduce scar formation following cleft repair. Orofacial clefting can be diagnosed in the fetus through prenatal ultrasound screening and allows planning for the harvesting of umbilical cord blood stem cells upon birth. Tissue engineering techniques using these cord blood stem cells and molecular targeting of inflammation and fibrosis during surgery may promote tissue regeneration. We expect that this novel strategy improves both muscle and skin regeneration, resulting in better function and esthetics after cleft repair.

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Human umbilical cord mesenchymal stem cell conditioned medium attenuates renal fibrosis by reducing inflammation and epithelial-to-mesenchymal transition via the TLR4/NF-κB signaling pathway in vivo and in vitro

Cited by 91 publications

References 31 publications

Are activated B cells involved in the process of myocardial fibrosis after acute myocardial infarction? -An in vivo experiment

Are activated B cells involved in the process of myocardial fibrosis after acute myocardial infarction? -An in vivo experiment

Mesenchymal stem cell-derived secretomes for therapeutic potential of premature infant diseases

Tissue engineering strategies combining molecular targets against inflammation and fibrosis, and umbilical cord blood stem cells to improve hampered muscle and skin regeneration following cleft repair

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