Human Turbinate-derived Mesenchymal Stem Cells Differentiated into Keratocyte Progenitor Cells

Park, Moon Nyeo; Kim, Bonglee; Kim, Hyeon Ji; Park, Sun Hwa; Lim, Mi-Hyun; Choi, Yeong Jin; Yi, Hee; Jang, Jinah; Kim, Sung Won; Cho, Dong‐Woo

doi:10.4172/2155-9570.1000627

Cited by 2 publications

(2 citation statements)

References 35 publications

(50 reference statements)

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“…Bioink consists of decellularized corneal ECM (Co-dECM) hydrogel encapsulating differentiated keratocytes. Human turbinate derived mesenchymal stem cells (hTMSCs) were prepared as previously described in detail [24]. Briefly, hTMSCs were obtained from the Catholic University of Korea, St. Mary's Hospital and cultured in normal Dulbecco's modified Eagle's medium (Gibco, USA) containing 10% (v/v) fetal bovine serum (Gibco, USA) and 1% (v/v) penicillin/streptomycin (Sigma-Aldrich, USA) at 37 °C in a humidified 5% CO 2 atmosphere.…”

Section: Preparation Of Bioinkmentioning

confidence: 99%

“…After preparing the samples using a SYBR Green PCR Master Mix assay (Applied Biosystems, USA), real-time PCR was conducted using an ABI 7500 Realtime PCR system (Applied Biosystems, USA). Primers were designed based on the previous study [24]. After denaturation at 95 °C for 10 min, the amplification reaction was conducted for 40 cycles of annealing at 95 °C for 15 s and extension and detection at 60 °C for 1 min.…”

Section: Gene Expression Analysismentioning

confidence: 99%

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Shear-induced alignment of collagen fibrils using 3D cell printing for corneal stroma tissue engineering

et al. 2019

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The microenvironments of tissues or organs are complex architectures comprised of structural proteins including collagen. Particularly, the cornea is organized in a lattice pattern of collagen fibrils which play a significant role in its transparency. This paper introduces a transparent bioengineered corneal structure for transplantation. The structure is fabricated by inducing shear stress to a corneal stroma-derived decellularized extracellular matrix bioink based on a 3D cell printing technique. The printed structure recapitulates the native macrostructure of the cornea with aligned collagen fibrils which results in the construction of a highly matured and transparent cornea stroma analog. The level of shear stress, controlled by the various size of the printing nozzle, manipulates the arrangement of the fibrillar structure. With proper parameter selection, the printed cornea exhibits high cellular alignment capability, indicating a tissue-specific structural organization of collagen fibrils. In addition, this structural regulation enhances critical cellular events in the assembly of collagen over time. Interestingly, the collagen fibrils that remodeled along with the printing path create a lattice pattern similar to the structure of native human cornea after 4 weeks in vivo. Taken together, these results establish the possibilities and versatility of fabricating aligned collagen fibrils; this represents significant advances in corneal tissue engineering.

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Section: Preparation Of Bioinkmentioning

confidence: 99%

Section: Gene Expression Analysismentioning

confidence: 99%