Human T lymphocyte receptor for sheep erythrocytes: Characterization of a specific antiserum and its application in the detection and quantitation of the receptor in a soluble form

Mendes, Nelson F.; Bernd, Luiz Antonio Guerra; Cintra, Heloisa H.A.; Mendes, Caio; Praça, Cristiano L.; Moura, Nayla C.

doi:10.1016/0008-8749(82)90291-x

Cited by 12 publications

(8 citation statements)

References 17 publications

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“…"Sensitized" E (ERs) was washed three times in cold Hanks' balanced salt solution (HBSS) at pH 7.2. The immunization schedule has been described by us elsewhere (13).…”

Section: Anti-e-receptor Serum (Anti-rs)mentioning

confidence: 99%

“…This antiserum is cytotoxic for T cells, inhibits Erosette formation, agglutinates ERs complexes, identifies T lymphocytes by immunofluorescence, and shows a single precipitation line in gel diffusion with preparations containing Rs (13,14).…”

Section: Introductionmentioning

confidence: 97%

“…We have developed several methods for quantitation of Rs in our laboratory (13,14) and the most practical is "rocket electrophoresis" (electroimmunodiffusion). With this method we have found abnormally high levels of Rs in the serum from patients with diseases associated with depression of cellmediated immunity such as leukemia, lymphoma, carcinoma, uremia, leprosy, and bone marrow aplasia (15)(16)(17).…”

Section: Introductionmentioning

confidence: 99%

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Quantitation of soluble E-Receptor of T lymphocytes in serum from HIV-1 positive patients

Nuñez

Moura

Hernández

et al. 1997

J. Clin. Lab. Anal.

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“…"Sensitized" E (ERs) was washed three times in cold Hanks' balanced salt solution (HBSS) at pH 7.2. The immunization schedule has been described by us elsewhere (13).…”

Section: Anti-e-receptor Serum (Anti-rs)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Quantitation of soluble E-Receptor of T lymphocytes in serum from HIV-1 positive patients

Nuñez

Moura

Hernández

et al. 1997

J. Clin. Lab. Anal.

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“…[5] To determine the level of the soluble E-receptor (SER) in different pathological conditions, sheep antiserum to the E-receptor (ERSA) was prepared by immunization of the sheep with the E-receptor coated autologous erythrocytes. [6] An elevated serum level of SER and CD2 was found in HIV-1 positive and AIDS patients. [7,8] We observed an increased serum level of SER in 60% of gastric carcinoma patients and in only 5% of donors.…”

Section: Introductionmentioning

confidence: 97%

“…EXPERIMENTAL Antisera ERSA was produced as described by Mendes et al, [6] with some modifications. [5] Rabbit antisera to such human plasma proteins as fibrinogen, haptoglobins (Dakopatts, Denmark), fibronectin, sheep antiserum to IgG (HþL chains) (Institute of Molecular Pathology, Estonia), apo-B100 and apo-A1 lipoproteins (Orion Diagnostica, Espoo, Finland) were used.…”

Section: Introductionmentioning

confidence: 99%

Association of CD2 with Fibrinogen in Human Plasma: Depletion of the Soluble E‐Receptor in Blood Clotting

Smorodin

Kurtenkov

Shevchuk

2007

Journal of Immunoassay and Immunochemistry

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The soluble E-receptor (SER) of lymphocytes that is related to CD2 was detected in human plasma and serum using immunoelectrophoresis with sheep antiserum. All plasma samples (n=18) demonstrated reactivity with antiserum, whereas the reactivity of the corresponding sera remained low or undetectable. The depletion of SER in clotting is associated with fibrinogen, as shown by crossed-affinity immunoelectrophoresis with antisera to plasma proteins. The SER-associated fibrinogen was purified and analysed by the SDS-polyacrylamide gel electrophoresis and immunoblotting. A band close to 66 kDa was detected with monoclonal antibodies to CD2. The association of CD2 and other soluble receptors with fibrinogen via domains is suggested. It is recommended that the fresh plasma, not serum, should be used to study circulating receptors because coagulation may appreciably diminish their physiological level in blood samples.

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Molecular forms of soluble human t lymphocyte receptor for sheep erythrocytes in serum and saliva

Itano

Ono

Sumigawa

et al. 1991

Clinical Laboratory Analysis

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Using a specific serum anti-soluble T lymphocytes receptor for sheep erythrocytes (E) and SDS-PAGE, we detected radioactive bands of molecular weight 58,000 in immunoprecipitates of supernatant of heated human lymphocytes (SHL), in the supernatant of PHA stimulated lymphocyte cultures (SLC), normal human serum (NHS), and serum from cancer and uremia patients, labelled with 131I. By Sephadex G-200 chromatography, in addition to this fraction, we detected molecules of molecular weight higher than 150,000 which interact with the anti-soluble receptor serum (anti-RS), in serum from cancer and uremia patients. These molecules were detected in NHS or SHL after concentration or by prolonged exposure of SDS-PAGE with some labelled and immunoprecipitated SHL samples. The soluble receptors of molecular weights 58,000 (RS1) and more than 150,000 (RS2) were fully identical when analyzed by immunodiffusion with anti-RS serum. When submitted to immunoelectrophoresis, RS1 showed electrophoretic migration similar to that of albumin, while RS2 showed a pattern close to that of alpha 2-globulin. However, RS2 did not show antigenic relationship with IgM and was not an immune complex with IgG. Even though the presence of RS in human saliva has not yet been reported, molecules that interact with anti-RS serum have been detected in human saliva and are fully identical to molecules found in supernatant of heated human T lymphocytes and NHS. The RS molecules present in human saliva have a molecular weight and electrophoretic migration similar to those of RS1 from SLC and from human serum and have no antigenic relationship with human albumin.

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Human T lymphocyte receptor for sheep erythrocytes: Characterization of a specific antiserum and its application in the detection and quantitation of the receptor in a soluble form

Cited by 12 publications

References 17 publications

Quantitation of soluble E-Receptor of T lymphocytes in serum from HIV-1 positive patients

Quantitation of soluble E-Receptor of T lymphocytes in serum from HIV-1 positive patients

Association of CD2 with Fibrinogen in Human Plasma: Depletion of the Soluble E‐Receptor in Blood Clotting

Molecular forms of soluble human t lymphocyte receptor for sheep erythrocytes in serum and saliva

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