Human Sulfide:Quinone Oxidoreductase Catalyzes the First Step in Hydrogen Sulfide Metabolism and Produces a Sulfane Sulfur Metabolite

Jackson, Michael R.; Melideo, Scott L.; Jörns, Marilyn Schuman

doi:10.1021/bi300778t

Cited by 245 publications

(326 citation statements)

References 60 publications

(115 reference statements)

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“…As shown in Figure 3, only H 2 S n (at 10 μM) elicited a dramatic increase in the fluorescence intensity of Mito-ss. Some other RSS, such as Cys, Hcy, GSH, CysSSCys, GSSG, NaHS, Cys-poly sulfide, S 8 , did not trigger any fluorescence enhancement. In addition, Mito-ss was also considerably inert to other reductive species such as ascorbic acid and tocopherol.…”

Section: ■ Results and Discussionmentioning

confidence: 90%

Near-Infrared Fluorescent Probe for Imaging Mitochondrial Hydrogen Polysulfides in Living Cells and in Vivo

et al. 2015

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Hydrogen polysulfides (H 2 S n , n > 1), derived from hydrogen sulfide (H 2 S), have attracted increasing attention in biochemical research, which may perform as the actual signaling molecules during cell signaling processes. Because of the closed biological and chemical relationship between H 2 S and H 2 S n , it is of great value to develop sensitive and specific techniques to distinguish the intracellular level of H 2 S n . To improve the understanding of the physiological and pathological roles played by H 2 S n , we now develop a specific fluorescent probe Mito-ss for capturing H 2 S n in cells and in vivo. When triggered by H 2 S n , Mito-ss replies a turn-on fluorescence signal and exhibits a higher selectivity toward H 2 S n than other abundant competing biothiols, such as glutathione, cysteine and H 2 S. The probe Mito-ss can also be applied to visual H 2 S n in living cells, as well as in vivo, providing a potentially powerful approach for probing H 2 S n in biological systems.

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Section: ■ Results and Discussionmentioning

confidence: 90%

Near-Infrared Fluorescent Probe for Imaging Mitochondrial Hydrogen Polysulfides in Living Cells and in Vivo

et al. 2015

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“…One electron from each of the two H 2 S are fed via the quinone pool (Q) into the respiratory chain (98) and they ultimately reduce O 2 at complex IV. SQR is bound to the inner mitochondrial membrane, and it is believed that sulfur is shuttled from SQR by an as-yet unidentified mobile carrier, possibly glutathione (GSH), dihydrolipoate, Trx, or sulfite (59,63,165). SQR is found in all tissues except the brain (58,82,89) (but see ''2.…”

Section: Metabolic Relationships Between H 2 S and Omentioning

confidence: 99%

Hydrogen sulfide as an oxygen sensor

Olson

2013

Clinical Chemistry and Laboratory Medicine

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Significance: Although oxygen (O 2 )-sensing cells and tissues have been known for decades, the identity of the O 2 -sensing mechanism has remained elusive. Evidence is accumulating that O 2 -dependent metabolism of hydrogen sulfide (H 2 S) is this enigmatic O 2 sensor. Recent Advances: The elucidation of biochemical pathways involved in H 2 S synthesis and metabolism have shown that reciprocal H 2 S/O 2 interactions have been inexorably linked throughout eukaryotic evolution; there are multiple foci by which O 2 controls H 2 S inactivation, and the effects of H 2 S on downstream signaling events are consistent with those activated by hypoxia. H 2 S-mediated O 2 sensing has been demonstrated in a variety of O 2 -sensing tissues in vertebrate cardiovascular and respiratory systems, including smooth muscle in systemic and respiratory blood vessels and airways, carotid body, adrenal medulla, and other peripheral as well as central chemoreceptors. Critical Issues: Information is now needed on the intracellular location and stoichometry of these signaling processes and how and which downstream effectors are activated by H 2 S and its metabolites. Future Directions: Development of specific inhibitors of H 2 S metabolism and effector activation as well as cellular organelle-targeted compounds that release H 2 S in a timeor environmentally controlled way will not only enhance our understanding of this signaling process but also provide direction for future therapeutic applications. Antioxid. Redox Signal. 22, 377-397.

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“…26,27 We hypothesize that the mitochondrial redox state may have closed relationship between H 2 S n and O 2…”

Section: Introductionmentioning

confidence: 99%

Near-Infrared Fluorescence Probe for in Situ Detection of Superoxide Anion and Hydrogen Polysulfides in Mitochondrial Oxidative Stress

et al. 2016

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H 2 S plays important physiological and pathological roles in cardiovascular system and nervous system. But recent evidences imply that hydrogen polysulfides (H 2 S n ) are the actual signaling molecules in cells. Although H 2 S n have been demonstrated to be responsible for mediating tumor suppressors, ion channels, and transcription factors, more of their biological effects are still need to be elaborated. On one hand, H 2 S n have been suggested to be generated from endogenous H 2 S upon reaction with reactive oxygen species (ROS). On the other hand, H 2 S n derivatives are proposed to be a kind of direct antioxidant against intracellular oxidative stress. This conflicting results should be attributed to the regulation of redox homeostasis between ROS and H 2 S n . Superoxide anion (O 2 •− ) is undoubtedly the primary ROS existing in mitochondria. We reason that the balance of O 2•− and H 2 S n are pivotal in physiological and pathological processes. Herein, we report two near-infrared fluorescent probes Hcy-Mito and Hcy-Biot for the detection of O 2•− and H 2 S n in cells and in vivo. Hcy-Mito is conceived to be applied in mitochondria, and Hcy-Biot is designed to target tumor tissue. Both of the probes were successfully applied for visualizing exogenous and endogenous O 2•− and H 2 S n in living cells and in tumor mice models. The results demonstrate that H 2 S n can be promptly produced by mitochondrial oxidative stress. Flow cytometry assays for apoptosis suggest that H 2 S n play critical roles in antioxidant systems.

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Human Sulfide:Quinone Oxidoreductase Catalyzes the First Step in Hydrogen Sulfide Metabolism and Produces a Sulfane Sulfur Metabolite

Cited by 245 publications

References 60 publications

Near-Infrared Fluorescent Probe for Imaging Mitochondrial Hydrogen Polysulfides in Living Cells and in Vivo

Near-Infrared Fluorescent Probe for Imaging Mitochondrial Hydrogen Polysulfides in Living Cells and in Vivo

Hydrogen sulfide as an oxygen sensor

Near-Infrared Fluorescence Probe for in Situ Detection of Superoxide Anion and Hydrogen Polysulfides in Mitochondrial Oxidative Stress

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