Human smooth muscle myosin heavy chain gene mapped to chromosomal region 16q12

Abstract

The partial nucleotide sequence encoding the rod portion of the entire amino acid sequence of human smooth muscle myosin heavy chain (MHC) which corresponds to MYH11, according to Human Gene Mapping nomenclature, has been determined by cloning a complementary DNA (cDNA) and sequencing the cDNA (UMYHSM). Northern blot analysis with the UMYHSM fragment (4.3 Kb) showed that the smooth muscle MHC of the human umbilical artery is expressed in the human umbilical artery, bladder, esophagus and trachea. Southern blot analysis of human genomic DNA from human-mouse or human-Chinese hamster somatic cell hybrids demonstrated that the human smooth muscle MHC was mapped to human chromosome 16. Regional mapping of UMYHSM was performed using human cell lines with partial deletion and trisomy of chromosome 16. As a result, the human smooth muscle MHC gene segregated with 16p11-q12. In situ hybridization of biotin-labeled human smooth muscle MHC probe (UMYHSM fragment) to normal human metaphase chromosome independently showed that the human smooth muscle MHC gene (MYH11) is assigned to chromosome region 16q12. Analysis of early metaphase chromosomes showed that hybridization signals were in 16q12.1. In the human, although skeletal, cardiac, smooth muscle, and nonmuscle MHC genes are mapped to chromosomes 17, 14, 16, and 22, respectively, structural similarities of these MHC genes strongly suggest the common origin of these genes.

Mimicry between the hepatitis C virus polyprotein and antigenic targets of nuclear and smooth muscle antibodies in chronic hepatitis C virus infection

“…We provide evidence for immunological cross-reactivity as a mechanism for the generation of SMA and ANA in chronic HCV [11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30] 2·11 ± 0·61 2·09 ± 0·49 0·97 HCV-pol [1479][1480][1481][1482][1483][1484][1485][1486][1487][1488][1489][1490][1491][1492][1493][1494][1495][1496][1497][1498] 2·33 ± 0·63 2·72 ± 0·95 0·14 Matrin [722][723][724][725][726][727][728][729][730][731][732][733][734][735][736][737][738]…”

“…Computer-assisted protein database search revealed six human proteins with high local sequence similarities with the HCV polyprotein: three nuclear proteins, namely, histone H2A.Z [22], matrin 3 [23] and replication protein A [24] and three smooth muscle proteins, namely, smooth-muscle myosin [25], vimentin [26] and smoothelin [27]. Of the nuclear proteins, histone H2A.Z and matrin 3 serve as structural components within the nucleus, while replication protein A is involved in DNA replication.…”

Pro-Inflammatory Cytokines Induce Expression of Matrix-Metabolizing Enzymes in Human Cervical Smooth Muscle Cells

“…18 Using primers to specifically amplify transcripts for SM1 and SM2, myosin heavy chain isoforms that are different at the Cterminal region, 19,20 we found that the CSMC expressed predominantly SM1. The analysis of myosin light chain transcripts indicated that the cultured CSMC had a light chain pattern consisting of 74% LC17b, which is smooth muscle-specific, and 26% of the LC17a isoform, which is expressed by both smooth muscle and non-muscle cells in humans.…”

Real-time quantitation of minimal residual disease in inv(16)-positive acute myeloid leukemia may indicate risk for clinical relapse and may identify patients in a curable state

“…5,6 It generates a chimeric mRNA transcript, CBF␤-MYH11, by fusing the CBF␤ gene located on band q22 of chromosome 16 7,8 and the MYH11 gene, which encodes a heavy chain of the smooth-muscle myosin protein, located at the p13 breakpoint. 9,10 Messenger RNA analysis by reverse transcription-polymerase chain reaction (RT-PCR) can help define leukemic subsets and provide potentially valuable diagnostic and prognostic information. 11 Qualitative RT-PCR reveals the presence of detectable levels of minimal residual disease (MRD) in patients in complete clinical remission (CR) after induction chemotherapy.…”