Journal of Chromatography A 2012 DOI: 10.1016/j.chroma.2011.10.059 View full text
|
|
Share
Mio Iwasaki, Naoyuki Sugiyama, Nobuo Tanaka, Yasushi Ishihama

Abstract: We have developed one-dimensional liquid chromatography-tandem mass spectrometry systems with meter-scale reversed phase monolithic silica-C₁₈ capillary columns for human proteome analysis. When tryptic peptides from 4 μg HeLa cell lysate proteins were directly injected onto a 4-m, 100 μm i.d. monolithic silica-C₁₈ column and an 8-h gradient was applied at 500 nL/min, 41,319 non-redundant tryptic peptides from 5,970 proteins were successfully identified from quadruplicate measurements; this is the best result …

expand abstract