Human Platelet-Derived Transforming Growth Factor-βStimulates Parameters of Bone Growth in Fetal Rat Calvariae*

Centrella, Michael; Massagué, Joan; Canalis, Ernesto

doi:10.1210/endo-119-5-2306

Cited by 190 publications

(62 citation statements)

References 26 publications

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“…Interleukin-1, which has limited amino acid sequence homology with acidic FGF (31), stimulates bone resorption and has stimulatory effects on collagen synthesis in 24-h cultures, whereas in 96 h cultures it is inhibitory and these effects are temporarily opposite to those of ECGF (32,33). Transforming growth factor-,B is the only growth factor that has effects resembling those of ECGF on bone formation, but it has been shown to stimulate bone resorption in mouse calvariae, an effect not reported in fetal rat long bones (26,34).…”

Section: Discussionmentioning

confidence: 99%

Effects of endothelial cell growth factor on bone remodelling in vitro.

Canalis¹,

Lorenzo²,

Burgess³

et al. 1987

J. Clin. Invest.

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Endothelial cell growth factor (ECGF) a was studied for its effects on bone formation in cultured fetal rat calvariae and on bone resorption in cultured fetal rat long bones.ECGF at 0.1-100 ng/ml stimulated I3Hlthymidine incorporation into DNA, an effect enhanced by heparin. Treatment with ECGF for 24 h decreased the incorporation of V3Hlproline into collagen but treatment for 48-96 h increased collagen and noncollagen protein synthesis, an effect that was concomitant with an increase in DNA content. ECGF did not alter collagen degradation in calvariae or 4"Ca release from long bones, which indicated it had no effect on bone resorption. Although ECGF increased prostaglandin E2 concentrations, its effect on DNA synthesis was not prostaglandin-mediated.In conclusion, ECGF stimulates calvarial DNA synthesis, which is an effect that results in a generalized increase in protein synthesis, but ECGF has no effect on matrix degradation or bone resorption.

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Section: Discussionmentioning

confidence: 99%

Effects of endothelial cell growth factor on bone remodelling in vitro.

Canalis¹,

Lorenzo²,

Burgess³

et al. 1987

J. Clin. Invest.

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“…TGF-P has also been shown to promote cartilage and bone growth when introduced subperiosteally (Noda and Camilliere, 1989;Joyce et al, 1990). The target of such TGF-p action may be mesenchymal cells, chondrocytes, preosteoblast, osteoblasts, and/or osteocytes (Centrella, 1986;Sporn and Roberts, 1990;Noda and Camilliere, 1989;Joyce et al, 1990;Massague, 1990). It has been shown that TGF-P acts through regulation of ECM synthesis (Rizzino, 1988;Massague, 1990;Sporn and Roberts, 1990), e.g., increasing the expression of fibronectin (Ignotz and Massague, 1986;Dean et al, 1988) and collagen (Seyedin et al, 1987;Ingotz and Massague, 1986;Roberts et al, 1986;Varga et al, 1987).…”

Section: Introductionmentioning

confidence: 99%

Effect of systemic calcium deficiency on the expression of transforming growth factor‐β in chick embryonic calvaria

Sato

Tuan

1992

Developmental Dynamics

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The developmental process of intramembranous ossification involves bone formation directly from mesenchymal differentiation without a cartilage intermediate. We have previously observed that systemic calcium deficiency in the developing chick embryo, produced by long-term shell-less culture, results in the appearance of chondrocyte-like cells in the calvarium, a parietal bone which normally develops via intramembranous ossification. This investigation aims to analyze the mechanism underlying this calcium deficiency-related, aberrant appearance of cartilage phenotype in the chick embryonic calvarium. In view of the reported involvement of transforming growth factor p (TGF-P) in osteogenesis and chondrogenesis, we have examined and compared here the expression of TGF-p in the chick embryonic calvaria of normal (in ovo development, NL), shell-less (SL), and calcium-supplemented SL (SL + Ca) embryos. TGF-f3 expression was analyzed at the mRNA level by blot and in situ cDNA hybridization, and at the protein level by immunohistochemistry and immunoblotting. The results presented here indicate that: 1) TGF-p is expressed in the chick embryonic calvarium by both periosteal cells and osteocytes, as revealed by in situ hybridization and immunohistochemistry; 2) TGF-P expression is significantly increased in SL calvarium compared to NL calvarium, at both protein and mRNA levels; 3) the number of TGF-f3 expressing cells increases in the SL calvarium, particularly along the central, subcambial core region of the bone; and 4) exogenous calcium repletion to the SL embryo affects the expression of TGF-P such that the pattern approaches that in the NL embryo. Taken together, these results indicate that altered TGF-f3 expression accompanies the aberrant appearance of cartilage phenotype caused by systemic calcium deficiency. We postulate that normal cellular differentiation along the osteogenic pathway during embryonic intramembranous ossification is crucially dependent on regulated TGF-0 expression. o 1992 Wiley-Liss, Inc.

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“…For example, rhBMP-2 increases ALP activity in C26, W-20-17, MC3T3-E1 cells, whereas TGF-β1 inhibits it [4,11,22]. Further, TGF-β1 does not appreciably affect cells in organ culture of rat calvariae [3]. These results suggest that TGF-β1 may not be a suitable agent to induce differentiation in osteosarcoma cells.…”

Section: Discussionmentioning

confidence: 92%

“…β1 are varied because the effect of TGF-β1 depends on the characteristics of the cell, namely whether it is tumorderived, its species of origin, and the stage of cell differentiation [3,4,11]. For example, rhBMP-2 increases ALP activity in C26, W-20-17, MC3T3-E1 cells, whereas TGF-β1 inhibits it [4,11,22].…”

Section: Discussionmentioning

confidence: 99%

1, 25-Dihydroxyvitamin D3, Recombinant Human Transforming Growth Factor-β1, and Recombinant Human Bone Morphogenetic Protein-2 Induce In Vitro Differentiation of Canine Osteosarcoma Cells

Nozaki

Kadosawa

Nishimura

et al. 1999

The Journal of Veterinary Medical Science

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ABSTRACT. Effects of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), recombinant human transforming growth factor (rhTGF)-β1 and recombinant human bone morphogenetic protein (rhBMP)-2 on differentiation in four different canine osteosarcoma cell lines (POS53B, 53C, 53D and 14A) were examined using markers specifically expressed by phenotypic osteoblasts. 1,25(OH)2D3 increased alkaline phosphatase (ALP) activity in one cell line, osteocalcin production in two lines and type I collagen production in three lines. RhTGF-β1 increased ALP activity in one clonal cell, osteocalcin production in one clonal cell and type I collagen production in two clonal cells. RhBMP-2 increased ALP activity in all clonal cells, osteocalcin production in two clonal cells and type I collagen production in three clonal cells. Thus, these agents induced differentiation in osteosarcoma cells at different efficacies. Electron microscopic study revealed that these agents increased cellular activity in all cell lines with no evidence of degeneration of cell organelle by drug cytotoxicity. In some cultures treated with either 1,25(OH)2D3 or rhBMP-2, apoptotic cells were observed. Based on the change in markers, rhBMP-2 and 1,25(OH)2D3 seemed to be more effective than rhTGF-β1. These agents are potential inducers of apoptosis.-KEY WORDS: BMP-2, differentiation, osteosarcoma, TGF-β1, vitamin D3.J. Vet. Med. Sci. 61(6): 649-656, 1999 1,25(OH)2D3 also enhanced antitumor chemotherapy both in vitro and in vivo [32]. TGF-β1, one of the most abundant growth regulatory factors in bone matrix, has complex effects. These include growth inhibition as well as a stimulation of both ALP activity and the production of bone matrix proteins such as collagen, osteonectin and fibronectin in osteoblasts [23]. BMPs (BMP-2 to BMP-15) belong to the TGF-β superfamily, and BMP-2, 3, 4 and 7 induce or enhance osteoblast differentiation [36]. Previously, four separate sublines of cells were established from the POS canine osteosarcoma cell line [10,24]. These sublines are immortal and vary from one another in morphology and ALP activity. The purpose of this study is to clarify the effects of 1,25(OH)2D3, TGF-β1 and BMP-2 on differentiation in these osteosarcoma cell lines. MATERIALS AND METHODS Cells:Four canine osteosarcoma cell sublines (POS53B, 53C, 53D, and 14A) were obtained from a POS parental cell line by limiting dilution as described previously [24]. POS53B forms chondroblastic-type tissue when transplanted into nude mice. Similarly, POS53C, 53D, and 14A form undifferentiated-, osteoblastic-, and fibroblastic-type tissues, respectively. Each cell line was maintained in RPMI 1640 medium (Nissui Seiyaku Co., Tokyo) supplemented with 10% fetal bovine serum (FBS) (Cell Culture Lab., Cleveland, OH), L-glutamine (Nissui) and antibiotics (50 mg/l gentamycin sulfate and 1.5 mg/l amphotericin B) at 37°C in a humidified atmosphere of 5% CO2.

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Human Platelet-Derived Transforming Growth Factor-βStimulates Parameters of Bone Growth in Fetal Rat Calvariae*

Cited by 190 publications

References 26 publications

Effects of endothelial cell growth factor on bone remodelling in vitro.

Effects of endothelial cell growth factor on bone remodelling in vitro.

Effect of systemic calcium deficiency on the expression of transforming growth factor‐β in chick embryonic calvaria

1, 25-Dihydroxyvitamin D<sub>3</sub>, Recombinant Human Transforming Growth Factor-β<sub>1</sub>, and Recombinant Human Bone Morphogenetic Protein-2 Induce In Vitro Differentiation of Canine Osteosarcoma Cells

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Human Platelet-Derived Transforming Growth Factor-βStimulates Parameters of Bone Growth in Fetal Rat Calvariae*

Cited by 190 publications

References 26 publications

Effects of endothelial cell growth factor on bone remodelling in vitro.

Effects of endothelial cell growth factor on bone remodelling in vitro.

Effect of systemic calcium deficiency on the expression of transforming growth factor‐β in chick embryonic calvaria

1, 25-Dihydroxyvitamin D<sub>3</sub>, Recombinant Human Transforming Growth Factor-&beta;<sub>1</sub>, and Recombinant Human Bone Morphogenetic Protein-2 Induce In Vitro Differentiation of Canine Osteosarcoma Cells

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Resources

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1, 25-Dihydroxyvitamin D<sub>3</sub>, Recombinant Human Transforming Growth Factor-β<sub>1</sub>, and Recombinant Human Bone Morphogenetic Protein-2 Induce In Vitro Differentiation of Canine Osteosarcoma Cells