Human monocyte/macrophage adhesion and cytokine production on surface‐modified poly(tetrafluoroethylene/hexafluoropropylene) polymers with and without protein preadsorption

Yun, Jong K.; DeFife, Kristin M.; Colton, Erica; Stack, S.; Azeez, A.; Cahalan, Linda; Verhoeven, Marjolein; Cahalan, Patrick T.; Anderson, James M.

doi:10.1002/jbm.820290217

Cited by 67 publications

(47 citation statements)

References 26 publications

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“…In our study, collagen I coatings enhanced alkaline phosphatase and OCN expression levels, triggering increased osteogenic behavior, whereas laminin-1 coatings increased EGF, nestin, cadherin 1, and keratin 8 expression, directing cells toward neurogenic cell behavior. These findings confirm previous data on the effect of surface coatings on cell function [27][28][29] and suggest that surface coatings may be used to further enhance the regenerative potential of periodontal progenitors.…”

Section: Discussionsupporting

confidence: 90%

Successful Periodontal Ligament Regeneration by Periodontal Progenitor Preseeding on Natural Tooth Root Surfaces

Dangaria

Ito

Diekwisch

2011

Stem Cells and Development

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Section: Discussionsupporting

confidence: 90%

Successful Periodontal Ligament Regeneration by Periodontal Progenitor Preseeding on Natural Tooth Root Surfaces

Dangaria

Ito

Diekwisch

2011

Stem Cells and Development

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“…[109][110][111][112][113][114][115][116][117] These studies have shown that macrophage activation can be modulated by the surface properties, such as material surface chemistry and surface topography [118], of the biomedical polymer.…”

Section: Crosstalk Between Macrophages/fbgcs and Inflammatorymentioning

confidence: 99%

Foreign body reaction to biomaterials

Anderson

Rodriguez

Chang

2008

Seminars in Immunology

4,172

4,024

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The foreign body reaction composed of macrophages and foreign body giant cells is the end-stage response of the inflammatory and wound healing responses following implantation of a medical device, prosthesis, or biomaterial. A brief, focused overview of events leading to the foreign body reaction is presented. The major focus of this review is on factors that modulate the interaction of macrophages and foreign body giant cells on synthetic surfaces where the chemical, physical, and morphological characteristics of the synthetic surface are considered to play a role in modulating cellular events. These events in the foreign body reaction include protein adsorption, monocyte/ macrophage adhesion, macrophage fusion to form foreign body giant cells, consequences of the foreign body response on biomaterials, and cross-talk between macrophages

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“…The surface onto which this cell population adheres has been shown to influence such secretion. 6,27,46 Our antibody array analysis revealed greater production of soluble factors by macrophages differentiated on RGD compared with those cultured on Ch-based films. Consistent with this, enhanced secretion of matrix metaloproteinases (MMPs) and TIMPs by RGD-differentiated macrophages, as well as of more cytokine production by RGD-adherent dendritic cells, has been previously described.…”

Section: Discussionmentioning

confidence: 83%

“…47 In addition, Yun et al showed that Fg preadsorbed onto poly(tetrafluoroethylene/ hexafluoropropylene) (FEP) copolymers induced higher monocyte adhesion on surfaces containing carboxyl groups compared with an FEP surface with only amine groups. 27 Moreover, preadsorption of Fg onto three distinct polymers, previously coated with poly(2-methacryloyloxyethyl phosphorylcholine [MCP]-co-n-butyl methacrylate [BMA]), affected macrophage adhesion differently, according to the substrate used. Still, the number of adherent cells diminished in comparison to uncoated polymers and TCPS.…”

Section: Discussionmentioning

confidence: 99%

“…24,25 Preadsorption of Fg has been shown to have an important role in initiating inflammatory responses to implanted biomaterials and, in particular, in the regulation of the macrophage response. [26][27][28][29][30] Fg is also crucial in wound healing and repair. Fg( -/-) mice form granulation tissue morphologically similar to control animals, but less mechanically stable, with a consequent wound instability.…”

mentioning

confidence: 99%

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Adsorbed Fibrinogen Enhances Production of Bone- and Angiogenic-Related Factors by Monocytes/Macrophages

Maciel

Oliveira²,

Colton

et al. 2014

Tissue Engineering Part A

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Macrophages are phagocytic cells with great importance in guiding multiple stages of inflammation and tissue repair. By producing a large number of biologically active molecules, they can affect the behavior of other cells and events, such as the foreign body response and angiogenesis. Since protein adsorption to biomaterials is crucial for the inflammatory process, we addressed the ability of the pro-inflammatory molecule fibrinogen (Fg) to modulate macrophage behavior toward tissue repair/regeneration. For this purpose, we used chitosan (Ch) as a substrate for Fg adsorption. Freshly isolated human monocytes were seeded on Ch substrates alone or previously adsorbed with Fg, and allowed to differentiate into macrophages for 10 days. Cell adhesion and morphology, formation of foreign body giant cells (FBGC), and secretion of a total of 80 cytokines and growth factors were evaluated. Both substrates showed similar numbers of adherent macrophages along differentiation as compared with RGD-coated surfaces, which were used as positive controls. Fg did not potentiate FBGC formation. In addition, actin cytoskeleton staining revealed the presence of punctuate F-actin with more elongated and interconnecting cells on Ch substrates. Antibody array screening and quantification of inflammation-and wound-healing-related factors indicated an overall reduction in Ch-based substrates versus RGD-coated surfaces. At late times, most inflammatory agents were downregulated in the presence of Fg, in contrast to growth factor production, which was stimulated by Fg. Importantly, on Ch + Fg substrates, fully differentiated macrophages produced significant amounts of macrophage inflammatory protein-1delta (MIP-1d), platelet-derived growth factor-BB, bone morphogenetic protein (BMP)-5, and BMP-7 compared with Ch alone. In addition, other important factors involved in bone homeostasis and wound healing, such as growth hormone, transforming growth factor-b3, and insulin-like growth factor-binding proteins, as well as several angiogenic mediators, including endocrine gland-derived vascular endothelial factor, fibroblast growth factor-7, and placental growth factor, were significantly promoted by Fg. This work provides a new perspective on the inflammatory response in the context of bone repair/regeneration mediated by a pro-inflammatory protein (Fg) adsorbed onto a biomaterial (Ch) that does not otherwise exhibit osteogenic properties.

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Human monocyte/macrophage adhesion and cytokine production on surface‐modified poly(tetrafluoroethylene/hexafluoropropylene) polymers with and without protein preadsorption

Cited by 67 publications

References 26 publications

Successful Periodontal Ligament Regeneration by Periodontal Progenitor Preseeding on Natural Tooth Root Surfaces

Successful Periodontal Ligament Regeneration by Periodontal Progenitor Preseeding on Natural Tooth Root Surfaces

Foreign body reaction to biomaterials

Adsorbed Fibrinogen Enhances Production of Bone- and Angiogenic-Related Factors by Monocytes/Macrophages

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