Human Microbiota of the Argentine Population- A pilot study

Carbonetto, Belén; Fabbro, Mónica; Sciara, Mariela; Seravalle, Analía; Mejico, Guadalupe; Revale, Santiago; Romero, Soledad; Brun, Bianca; Fay, Marcelo; Fay, Fabián; Vázquez, Martı́n

doi:10.1101/030361

Cited by 4 publications

(4 citation statements)

References 30 publications

(10 reference statements)

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“…For instance, Firmicutes are decreased but Bacteroidetes have no changes in Colombian adults compared with Americans, Europeans and Asians (Escobar et al 2014;Magne et al 2016). Moreover, Ruminococcaceae, Lachnospiraceae, Rikenellaceae and Prevotellaceae are more abundant in Argentinan than Americans (Carbonetto et al 2016;Hansen et al 2019).…”

Section: Geographical Locationmentioning

confidence: 96%

Gut bacteria formation and influencing factors

Yang

et al. 2021

FEMS Microbiology Ecology

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The gut microbiota plays an important role in human health. In modern life, with the improvement of living conditions, the intake of high-sugar and high-fat diets as well as the large-scale use of antibacterial drugs have an extensive impact on the gut microbiota, even leading to gut microbiota-orchestrating disorders. This review discusses the effects of various factors, including geographic location, age, diet, antibacterial drugs, psychological situation and exercise on gut bacteria, which helps us profoundly to understand the significance of gut bacteria to human health and to find effective solutions to prevent or treat related diseases.

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Section: Geographical Locationmentioning

confidence: 96%

Gut bacteria formation and influencing factors

Yang

et al. 2021

FEMS Microbiology Ecology

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“…Overall, 25 cases and 25 controls (wo-HCC) were well matched according to those variables previously described and were included in the final assessement of gut microbiome (Figure 1). A subgroup of 25 healthy individuals were also enrolled, matched according to gender in a 1:1 ratio 26 . Baseline patient characteristics are shown on Table 1.…”

Section: Participating Patients Characteristicsmentioning

confidence: 99%

“A different gut microbiome linked to inflammation found in cirrhotic patients with and without hepatocellular carcinoma”

Piñero

Vázquez

Baré

et al. 2017

Preprint

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SUMMARYBackgroundNo specific microbiome in patients with hepatocellular carcinoma (HCC) has been reported to date.AimTo compare the gut microbiome found in cirrhotic patients with and without HCC.MethodsFrom 407 patients with Child Pugh A/B cirrhosis prospectively followed, 25 with HCC (cases) were matched with 25 without HCC (wo-HCC) in a 1:1 ratio according to age, gender, etiology, Child Pugh and severity of portal hypertension. In addition results were also compared with 25 healthy subjects. Faecal stool samples were collected noninvasively, aliquoted for DNA extraction and sequenced for the V3-V4 region of the microbial 16S rRNA (Illumina MiSeq Platform).ResultsThere were no significant clinical differences among cases and controls. We found a differential abundance in family members of Firmicutes with a 3-fold increased of Erysipelotrichaceae and a 5-fold decrease in family Leuconostocaceae in HCC when compared to wo-HCC controls. Genus Fusobacterium was found 5-fold decreased in HCC versus wo-HCC. The ratio bacteriodes/prevotella was increased in HCC due to the significant decrease in the genus prevotella. Genus Odoribacter and Butyricimonas were more differentialy abundant in HCC. This pattern has been previously associated with an inflammatory milieu with a putative increased activation of NOD-like receptor signalling pathways. A Random Forest model trained with differential abundant taxa correctly classifyed HCC individuals with an error of 22%.ConclusionsA pattern of microbiome linked to inflammation was observed in cirrhotic patients with HCC. These findings open the discussion whether or not microbiota has a physiopathologic role in HCC development in cirrhosis.

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“…In particular only three articles have been published studying the gut microbiome of different Argentine populations, two of them of our authorship. The first local pilot study reported the most relevant and abundant bacteria in samples from different body regions in individuals from Rosario, Santa Fe (Argentina) [21] . We recently published the microbial gut diversity of Buenos Aires (BA), being the first local report in this area [22] .…”

Section: Introductionmentioning

confidence: 99%

New insights in Ulcerative Colitis Associated Gut Microbiota in South American Population: Akkermansia and Collinsella, two distinctive genera found in Argentine subjects

Aguilera

Quesada

et al. 2020

Preprint

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Background Globally, ulcerative colitis (UC) is the most common form of intestinal inflammation, which is believed to be the result of a deregulated immune system response to commensal microbiota in a genetically susceptible host. Multicellular organisms rely heavily on their commensal symbiotic microbiota, whose composition is closely related to intrinsic local characteristics and regulated or modified by environmental factors. In the present study we aim to describe the unknown gut microbiota of patients with UC in comparison with healthy individuals in order to find novel biomarkers for UC in our region. Methods We evaluated 46 individuals, 26 healthy non-UC controls and 20 UC patients, from the metropolitan area of Buenos Aires (BA), Argentina. Clinical features, biochemical tests and anthropometric measurements were determined. Fecal samples were collected and DNA was extracted for microbiota analysis. The hypervariable regions V3-V4 of the bacterial 16SR gene were sequenced using a MiSeq platform and sequences were analyzed using the QIIME2 environment. In addition, we looked for differential functional pathways using PICRUSt and compared the performance of three machine learning models to discriminate the studied individuals, using taxa and functional annotations. Results All UC patients were under clinical treatment with 70% of individuals in remission. We found no significant differences in gut microbiota richness or evenness between UC patients and non-UC controls (alpha diversity). Remarcably, microbial compositional structure within groups (beta diversity) showed differences: At the phylum level, Verrucomicrobia was overrepresented in controls while Actinobacteria was distinctive of UC patients; At the genus level Bacteroides and Akkermancia were significantly more abundant among controls while Eubacterium and Collinsella in UC patients. In addition, our results showed that carbohydrates metabolism was preponderant in UC patients, not observing a distinctive biochemical pathway for the healthy non-UC controls. Finally, in order to define a robust classifying method in our population, we evaluated the capability of three machine learning models to classify individuals. Our results reinforced the idea of functional compensation in microbiome communities, as models that used KEGG orthologs annotations had better capabilities than taxonomy to distinguish UC patients. Conclusions Our study provides new knowledge on the differences and similarities of the gut microbiota of UC patients as compared to non-UC controls of our population. This allows not only the association of local changes in gut microbial diversity with the pathology process, but also the future development of personalized nutritional and pharmacological therapies through the use of omic strategies describing the metagenomic profiles of the Argentine population.

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Human Microbiota of the Argentine Population- A pilot study

Cited by 4 publications

References 30 publications

Gut bacteria formation and influencing factors

Gut bacteria formation and influencing factors

“A different gut microbiome linked to inflammation found in cirrhotic patients with and without hepatocellular carcinoma”

New insights in Ulcerative Colitis Associated Gut Microbiota in South American Population: Akkermansia and Collinsella, two distinctive genera found in Argentine subjects

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