Human mesenchymal stem cells differentiate into an osteogenic lineage in presence of strontium containing bioactive glass nanoparticles

Naruphontjirakul, Parichart; Tsigkou, Olga; Li, Siwei; Porter, Alexandra E.; Jones, Julian R.

doi:10.1016/j.actbio.2019.03.038

Cited by 83 publications

(63 citation statements)

References 106 publications

(137 reference statements)

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“…Nanoparticles containing as little as 4.4 mol% SrO (90.6 mol% SiO2, 5.0 mol% CaO, 4.4% mol% SrO) stimulated hMSCs to differentiated down an osteogenic route, where Sr-free nanoparticles did not differentiate [18].…”

Section: Introductionmentioning

confidence: 99%

Osteogenic potential of sol–gel bioactive glasses containing manganese

Barrioni

Norris

et al. 2019

J Mater Sci: Mater Med

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We report that the release of manganese ions from bioactive glass provoked human mesenchymal stem cell (hMSC) differentiation down a bone pathway, whereas hMSCs exposed to the Mn-free glass did not differentiate. Bioactive glasses (BGs) are widely used for bone regeneration, and allow the incorporation of different ions with therapeutic properties into the glass network. Amongst the different ions with therapeutic benefits, manganese (Mn) has been shown to influence bone metabolism and activate human osteoblasts integrins, improving cell adhesion, proliferation and spreading. Mn has also been incorporated into bioceramics as a therapeutic ion for improved osteogenesis. Here, up to 4.4 mol% MnO was substituted for CaO in the 58S composition (60 mol% SiO2, 36 mol% CaO, 4 mol% P2O5) and its effects on the glass properties and capability to influence the osteogenic differentiation were evaluated. Mncontaining BGs with amorphous structure, high specific surface area and nanoporosity were obtained. The presence of Mn 2+ species was confirmed by X-ray photoelectron spectroscopy (XPS). Mn-containing BGs presented no cytotoxic effect on human mesenchymal stem cells (hMSCs) and enabled sustained ion release in culture medium. hMSCs osteogenic differentiation stimulation and influence on the mineralisation process was also confirmed through the alkaline phosphatase (ALP) activity, and expression of osteogenic differentiation markers, such as collagen type I, osteopontin and osteocalcin, which presented higher expression in the presence of Mn-containing samples compared to control. Mn incorporation offers great promise for obtaining glasses with superior properties for bone tissue regeneration.

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Section: Introductionmentioning

confidence: 99%

Osteogenic potential of sol–gel bioactive glasses containing manganese

Barrioni

Norris

et al. 2019

J Mater Sci: Mater Med

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“…To enhance its biological activity, we added strontium to harness its therapeutic properties [ 9 , 10 ]. Although strontium's mechanism of action remains unknown, strontium-containing inorganic bone substitutes increase bone formation and osteointegration in vivo [ [14] , [15] , [16] , [17] , [18] , [19] , [20] ], and modulate the activity of osteoprogenitors, osteoblasts, and osteoclasts, and macrophage phenotype in vitro [ 11 , 12 , [53] , [54] , [55] , [56] ]. We have recently shown using a whole-genome microarray analysis that strontium incorporation in BG45S5 triggered significant changes in the global gene expression patterns of hMSCs and resulted in important changes in their metabolism and membrane composition [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

Multiscale analyses reveal native-like lamellar bone repair and near perfect bone-contact with porous strontium-loaded bioactive glass

et al. 2019

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The efficient healing of critical-sized bone defects using synthetic biomaterial-based strategies is promising but remains challenging as it requires the development of biomaterials that combine a 3D porous architecture and a robust biological activity. Bioactive glasses (BGs) are attractive candidates as they stimulate a biological response that favors osteogenesis and vascularization, but amorphous 3D porous BGs are difficult to produce because conventional compositions crystallize during processing. Here, we rationally designed a porous, strontium-releasing, bioactive glass-based scaffold (pSrBG) whose composition was tailored to deliver strontium and whose properties were optimized to retain an amorphous phase, induce tissue infiltration and encourage bone formation. The hypothesis was that it would allow the repair of a critical-sized defect in an ovine model with newly-formed bone exhibiting physiological matrix composition and structural architecture. Histological and histomorphometric analyses combined with indentation testing showed pSrBG encouraged near perfect bone-to-material contact and the formation of well-organized lamellar bone. Analysis of bone quality by a combination of Raman spectral imaging, small-angle X-ray scattering, X-ray fluorescence and focused ion beam-scanning electron microscopy demonstrated that the repaired tissue was akin to that of normal, healthy bone, and incorporated small amounts of strontium in the newly formed bone mineral. These data show the potential of pSrBG to induce an efficient repair of critical-sized bone defects and establish the importance of thorough multi-scale characterization in assessing biomaterial outcomes in large animal models.

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“…In particular, HA nanostructured with concave macroporosity, derived from CaP crystals, accelerate osteoinduction, since they are chemically and structurally similar to those of the natural bone tissue [ 52 ]. Other nanoparticles made by different components, such as molybdenum-doped bioactive glass [ 53 ], magnetic iron oxide [ 54 ], strontium containing bioactive glass [ 55 ], and gold [ 56 ] showed osteoinductive abilities.…”

Section: Bioprintingmentioning

confidence: 99%

Advances on Bone Substitutes through 3D Bioprinting

Genova

Roato

Carossa

et al. 2020

IJMS

101

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Reconstruction of bony defects is challenging when conventional grafting methods are used because of their intrinsic limitations (biological cost and/or biological properties). Bone regeneration techniques are rapidly evolving since the introduction of three-dimensional (3D) bioprinting. Bone tissue engineering is a branch of regenerative medicine that aims to find new solutions to treat bone defects, which can be repaired by 3D printed living tissues. Its aim is to overcome the limitations of conventional treatment options by improving osteoinduction and osteoconduction. Several techniques of bone bioprinting have been developed: inkjet, extrusion, and light-based 3D printers are nowadays available. Bioinks, i.e., the printing materials, also presented an evolution over the years. It seems that these new technologies might be extremely promising for bone regeneration. The purpose of the present review is to give a comprehensive summary of the past, the present, and future developments of bone bioprinting and bioinks, focusing the attention on crucial aspects of bone bioprinting such as selecting cell sources and attaining a viable vascularization within the newly printed bone. The main bioprinters currently available on the market and their characteristics have been taken into consideration, as well.

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Human mesenchymal stem cells differentiate into an osteogenic lineage in presence of strontium containing bioactive glass nanoparticles

Cited by 83 publications

References 106 publications

Osteogenic potential of sol–gel bioactive glasses containing manganese

Osteogenic potential of sol–gel bioactive glasses containing manganese

Multiscale analyses reveal native-like lamellar bone repair and near perfect bone-contact with porous strontium-loaded bioactive glass

Advances on Bone Substitutes through 3D Bioprinting

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