Human Mesenchymal Stem Cell Proliferation and Osteogenic Differentiation in Fibrin Gels in Vitro

Catelas, Isabelle; Sese, Nadjah; Wu, Benjamin M.; Dunn, James C.Y.; Helgerson, Sam; Tawil, Bill

doi:10.1089/ten.2006.12.ft-172

Cited by 14 publications

(16 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Disruption of either the HS or CS chains resulted in an increase in alkaline phosphatase mRNA expression. Osteocalcin is a late marker of osteogenic differentiation and has been shown in some studies to undergo no significant increase in differentiating hMSCs [53]. Similarly, here we found very low levels of osteocalcin upregulation during the increased osteogenic differentiation.…”

Section: Discussionsupporting

confidence: 81%

Disruption of Heparan and Chondroitin Sulfate Signaling Enhances Mesenchymal Stem Cell-Derived Osteogenic Differentiation via Bone Morphogenetic Protein Signaling Pathways

et al. 2007

View full text Add to dashboard Cite

Cell surface heparan sulfate (HS) and chondroitin sulfate (CS) proteoglycans have been implicated in a multitude of biological processes, including embryonic implantation, tissue morphogenesis, wound repair, and neovascularization through their ability to regulate growth factor activity and morphogenic gradients. However, the direct role of the glycosaminoglycan (GAG) sugar-side chains in the control of human mesenchymal stem cell (hMSC) differentiation into the osteoblast lineage is poorly understood. Here, we show that the abundant cell surface GAGs, HS and CS, are secreted in proteoglycan complexes that directly regulate the bone morphogenetic protein (BMP)-mediated differentiation of hMSCs into osteoblasts. Enzymatic depletion of the HS and CS chains by heparinase and chondroitinase treatment decreased HS and CS expression but did not alter the expression of the HS core proteins perlecan and syndecan. When digested separately, depletion of HS and CS chains did not effect hMSC proliferation but rather increased BMP bioactivity through SMAD1/5/8 intracellular signaling at the same time as increasing canonical Wnt signaling through LEF1 activation. Long-term culturing of cells in HS-and CS-degrading enzymes also increased bone nodule formation, calcium accumulation, and the expression of such osteoblast markers as alkaline phosphatase, RUNX2, and osteocalcin. Thus, the enzymatic disruption of HS and CS chains on cell surface proteoglycans alters BMP and Wnt activity so as to enhance the lineage commitment and osteogenic differentiation of hMSCs.

show abstract

Section: Discussionsupporting

confidence: 81%

Disruption of Heparan and Chondroitin Sulfate Signaling Enhances Mesenchymal Stem Cell-Derived Osteogenic Differentiation via Bone Morphogenetic Protein Signaling Pathways

et al. 2007

View full text Add to dashboard Cite

show abstract

“…The rationale for a cell culturing system based on fibrinogen is due to its ability to enhance cell attachment (Gorodetsky et al 1998), proliferation (Sporn et al 1995) and differentiation (Huang et al 2002). Several publications have judged fibrin to be a suitable scaffold material for colonization of human MSC (HMSC) (Catelas et al 2006;Trombi et al 2008) and HMSC are able to adhere, spread and proliferate, depending on different fibrinogen concentrations (Bensaid et al 2003). Perka et al (2001) developed a bioresorbable alginatefibrin vehicle that ensured an initial cell proliferation and differentiation to establish a stable matrix structure for transplantation of different cell types like periosteal cell and chondrocytes.…”

Section: Discussionmentioning

confidence: 99%

Platelet‐rich fibrin membranes as scaffolds for periosteal tissue engineering

Gaßling

Douglas

Warnke

et al. 2010

Clinical Oral Implants Res

186

141

View full text Add to dashboard Cite

show abstract

“…The fibrin matrix, as it has been used here from commercially available fibrin glue, also contains small amounts of fibronectin, which is an important ECM molecule (Malicev et al 2007). Fibronectin plays an important role in providing cells with a biomimetic microenvironment that can improve cell growth and increase DNA synthesis (Catelas et al 2006). In this way, the use of a fibrin matrix may permit the development of an environment that mimics physiologically important conditions, and obviously enhances the biological quality of a scaffold.…”

Section: Discussionmentioning

confidence: 99%

Human bone marrow stroma stem cell distribution in calcium carbonate scaffolds using two different seeding methods

Zhu

Schulz

Schliephake

2010

Clinical Oral Implants Res

View full text Add to dashboard Cite

In conclusion, automated serial optical sectioning using structured illumination FM can assess cell numbers and the 3D distribution of hBMSCs in mineralized scaffolds. This allows for a detailed analysis of the effect of different in vitro procedures used for cell seeding. The use of fibrin during seeding increases seeding efficiency and enhances both proliferation and cell survival in the central parts of the scaffolds.

show abstract

Human Mesenchymal Stem Cell Proliferation and Osteogenic Differentiation in Fibrin Gels in Vitro

Cited by 14 publications

References 34 publications

Disruption of Heparan and Chondroitin Sulfate Signaling Enhances Mesenchymal Stem Cell-Derived Osteogenic Differentiation via Bone Morphogenetic Protein Signaling Pathways

Disruption of Heparan and Chondroitin Sulfate Signaling Enhances Mesenchymal Stem Cell-Derived Osteogenic Differentiation via Bone Morphogenetic Protein Signaling Pathways

Platelet‐rich fibrin membranes as scaffolds for periosteal tissue engineering

Human bone marrow stroma stem cell distribution in calcium carbonate scaffolds using two different seeding methods

Contact Info

Product

Resources

About