Human liver GTP cyclohydrolase I: purification and some properties

GTPCyclohydrolase I: Purification, Characterization, and Effects ofInhibition on Nitric Oxide Synthase in Nocardia Species

“…Influences of calcium, iron, mercury, and zinc are similar to the effects of these cations on other GTPCHs, except that 5 mM CaCl 2 caused 5% inhibition on S. indica GTPCH (21). Concentrations greater than 1 mM for Zn 2ϩ inhibited purified GTPCHs from bacteria (4,21) and mammals (20,33), even though zinc is required for catalytic activity (2).…”

“…Instead, pyrrolopyrimidine is formed (11). GTPCHs involved in BH 4 biosynthesis have been purified to homogeneity from human, mouse, and rat tissues (15,20,33), as well as from Drosophila melanogaster (41). To date, no GTPCH enzymes have been characterized in actinomycetes.…”

GTPCyclohydrolase I: Purification, Characterization, and Effects ofInhibition on Nitric Oxide Synthase in Nocardia Species

“…Influences of calcium, iron, mercury, and zinc are similar to the effects of these cations on other GTPCHs, except that 5 mM CaCl 2 caused 5% inhibition on S. indica GTPCH (21). Concentrations greater than 1 mM for Zn 2ϩ inhibited purified GTPCHs from bacteria (4,21) and mammals (20,33), even though zinc is required for catalytic activity (2).…”

“…Instead, pyrrolopyrimidine is formed (11). GTPCHs involved in BH 4 biosynthesis have been purified to homogeneity from human, mouse, and rat tissues (15,20,33), as well as from Drosophila melanogaster (41). To date, no GTPCH enzymes have been characterized in actinomycetes.…”

GTPCyclohydrolaseI

“…Instead, pyrrolopyrimidine is formed (11). GTPCHs involved in BH 4 biosynthesis have been purified to homogeneity from human, mouse, and rat tissues (15,20,33), as well as from Drosophila melanogaster (41). To date, no GTPCH enzymes have been characterized in actinomycetes.…”

Biochemical characterization of the tetrahydrobiopterin synthesis pathway in the oleaginous fungus Mortierella alpina

“…GTPCH-1 and GTPCH-2 were most active in more alkaline conditions than the optimal pH ranges for human, D. melanogaster and Nocardia sp. GTPCHs (He & Rosazza, 2003;Shen et al, 1989;Weisberg & O'Donnell, 1986). PTPS had optimal activity at pH 7.0, which is similar to that for recombinant rat PTPS (optimal pH 6.5-7.0) (Inoue et al, 1991).…”

“…153) (Thöny et al, 2000). The genes responsible for the synthesis of BH 4 have been identified (Cha et al, 1991;Citron et al, 1990;Ohye et al, 1998;Shen et al, 1989;Thöny et al, 1992), and structural information for all pathway enzymes has been elucidated (Auerbach et al, 1997;Maita et al, 2004;Ploom et al, 1999).…”

GTPCyclohydrolase I: Purification, Characterization, and Effects ofInhibition on Nitric Oxide Synthase in Nocardia Species

“…Influences of calcium, iron, mercury, and zinc are similar to the effects of these cations on other GTPCHs, except that 5 mM CaCl 2 caused 5% inhibition on S. indica GTPCH (21). Concentrations greater than 1 mM for Zn 2ϩ inhibited purified GTPCHs from bacteria (4,21) and mammals (20,33), even though zinc is required for catalytic activity (2).…”

“…Instead, pyrrolopyrimidine is formed (11). GTPCHs involved in BH 4 biosynthesis have been purified to homogeneity from human, mouse, and rat tissues (15,20,33), as well as from Drosophila melanogaster (41). To date, no GTPCH enzymes have been characterized in actinomycetes.…”

GTPCyclohydrolaseI

“…Instead, pyrrolopyrimidine is formed (11). GTPCHs involved in BH 4 biosynthesis have been purified to homogeneity from human, mouse, and rat tissues (15,20,33), as well as from Drosophila melanogaster (41). To date, no GTPCH enzymes have been characterized in actinomycetes.…”

Biochemical characterization of the tetrahydrobiopterin synthesis pathway in the oleaginous fungus Mortierella alpina

“…GTPCH-1 and GTPCH-2 were most active in more alkaline conditions than the optimal pH ranges for human, D. melanogaster and Nocardia sp. GTPCHs (He & Rosazza, 2003;Shen et al, 1989;Weisberg & O'Donnell, 1986). PTPS had optimal activity at pH 7.0, which is similar to that for recombinant rat PTPS (optimal pH 6.5-7.0) (Inoue et al, 1991).…”

“…153) (Thöny et al, 2000). The genes responsible for the synthesis of BH 4 have been identified (Cha et al, 1991;Citron et al, 1990;Ohye et al, 1998;Shen et al, 1989;Thöny et al, 1992), and structural information for all pathway enzymes has been elucidated (Auerbach et al, 1997;Maita et al, 2004;Ploom et al, 1999).…”

GTPCyclohydrolase I: Purification, Characterization, and Effects ofInhibition on Nitric Oxide Synthase in Nocardia Species

“…Influences of calcium, iron, mercury, and zinc are similar to the effects of these cations on other GTPCHs, except that 5 mM CaCl 2 caused 5% inhibition on S. indica GTPCH (21). Concentrations greater than 1 mM for Zn 2ϩ inhibited purified GTPCHs from bacteria (4,21) and mammals (20,33), even though zinc is required for catalytic activity (2).…”

“…Instead, pyrrolopyrimidine is formed (11). GTPCHs involved in BH 4 biosynthesis have been purified to homogeneity from human, mouse, and rat tissues (15,20,33), as well as from Drosophila melanogaster (41). To date, no GTPCH enzymes have been characterized in actinomycetes.…”

GTPCyclohydrolaseI

“…Instead, pyrrolopyrimidine is formed (11). GTPCHs involved in BH 4 biosynthesis have been purified to homogeneity from human, mouse, and rat tissues (15,20,33), as well as from Drosophila melanogaster (41). To date, no GTPCH enzymes have been characterized in actinomycetes.…”

Biochemical characterization of the tetrahydrobiopterin synthesis pathway in the oleaginous fungus Mortierella alpina

“…GTPCH-1 and GTPCH-2 were most active in more alkaline conditions than the optimal pH ranges for human, D. melanogaster and Nocardia sp. GTPCHs (He & Rosazza, 2003;Shen et al, 1989;Weisberg & O'Donnell, 1986). PTPS had optimal activity at pH 7.0, which is similar to that for recombinant rat PTPS (optimal pH 6.5-7.0) (Inoue et al, 1991).…”

“…153) (Thöny et al, 2000). The genes responsible for the synthesis of BH 4 have been identified (Cha et al, 1991;Citron et al, 1990;Ohye et al, 1998;Shen et al, 1989;Thöny et al, 1992), and structural information for all pathway enzymes has been elucidated (Auerbach et al, 1997;Maita et al, 2004;Ploom et al, 1999).…”