Human Insulinlike Growth Factor 1 Gene Transfer Into Paralyzed Rat Larynx

Abstract

Applied to laryngeal paralysis, hIGF1 gene therapy provides an opportunity to augment surgical treatment modalities by the prevention or reversal of muscle atrophy, and enhancement of nerve sprouting and muscle reinnervation. Although the percentage of denervated muscles demonstrating hIGF1 expression was increased following multiple injections, no difference was observed in the biological response compared with that in the single-injection treatment groups. Further investigation will be conducted to assess long-term benefits and physiological responses and to define the limitations of this potentially valuable therapeutic strategy.

Remote Delivery of rAAV‐GFP to the Rat Brainstem Through the Recurrent Laryngeal Nerve

“…This study confirmed the neurotrophic and myotrophic effects of IGF‐I. 26 In their model, the transgene is expressed at the end organ (muscle). Retrograde transport of the vector is prevented by transection of the nerve.…”

Muscle Fiber and Satellite Cell Apoptosis in the Aging Human Thyroarytenoid Muscle: A Stereological Study with Confocal Laser Scanning Microscopy

“…For example, an age‐related decrease in insulin‐like growth factor I 18 probably contributes to the observed age‐related increase in apoptotic satellite cells, because growth factor withdrawal results in satellite cell apoptosis 19 . Consistent with these findings, viral‐mediated overexpression of insulin‐like growth factor I blocks the age‐related loss of muscle mass and strength in rat limb muscles 20 as well as denervation atrophy in the rat TA, 21 which is a function of myonuclear apoptosis 16 . Therefore, it may be possible to prevent or reverse age‐related muscle fiber loss and atrophy in the TA by using gene therapy or pharmacologic intervention to block the demonstrated age‐related increase in satellite cell and myonuclear apoptosis in this muscle.…”

Sendai virus transgene in a novel gene therapy for laryngotracheal disease

“…Most laryngeal mucosa consist of columnar epithelial cells; however, the vocal cord surface consists of squamous epithelial cells. Gene transduction into larynx has been reported using other viral vectors, but to our knowledge, ours is the first report of gene transduction into the squamous epithelium or the lamina propria of mucous membranes. The layered structure of the vocal fold lamina propria is essential for optimal mucosal vibration, and vocal fold scarring disrupts this structure.…”