Human infection associated with methicillin-resistant Staphylococcus pseudintermedius ST71

Stegmann, Ramona; Burnens, André P.; Maranta, Christian A.; Perreten, Vincent

doi:10.1093/jac/dkq241

Cited by 160 publications

(152 citation statements)

References 8 publications

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“…Due to the emergence and widespread dissemination of MRSP, it becomes an expensive and a serious challenge to control or eradicate these pathogenic biofilms using antibiotics. MRSP ST71 has been reported (Stegmann et al 2010) to cause human infection from companion animals demonstrating the therapeutic challenge and its zoonotic potential.…”

Section: Introductionmentioning

confidence: 99%

Atomic force microscopy study of the antibacterial effect of fosfomycin on methicillin-resistant Staphylococcus pseudintermedius

Neethirajan

DiCicco

2013

Appl Nanosci

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The influence of fosfomycin on methicillinresistant Staphylococcus pseudintermedius (MRSP) as the target cell was studied by atomic force microscopy (AFM). Nanoscale images of the effects of the antibiotic fosfomycin on this gram-positive bacterium's cell were obtained in situ without fixing agents. Our study has demonstrated substantial morphological and topographical differences between the control and fosfomycin-treated MRSP cells. The AFM investigations further revealed the rough surface morphology and a 30 % shrinkage in size of the fosfomycin-treated cell and the leakage of cytoplasmic components from the cell. The damage of cell membrane integrity and the cell surface degradation as observed elaborates the antibacterial activity of fosfomycin. The AFM image analysis also reveals that the fosfomycin inhibits cell division, and prevents the adhesion on the surface discouraging the biofilm attachment. The microtitre plate assay results conform to the atomic force microscopy image analysis. This is the first visual demonstration of the effect of fosfomycin on MRSP cells.

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Section: Introductionmentioning

confidence: 99%

Atomic force microscopy study of the antibacterial effect of fosfomycin on methicillin-resistant Staphylococcus pseudintermedius

Neethirajan

DiCicco

2013

Appl Nanosci

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“…It is the major cause of canine skin and ear infections [2,3]. Furthermore, human infections are sporadically reported [4,5], suggesting that, although it is estimated to be low, there is a threat of zoonotic disease. Approximately 30–35% of the S. pseudintermedius isolates tested in our University of Tennessee College of Veterinary Medicine Clinical Bacteriology Laboratory are methicillin-resistant.…”

Section: Introductionmentioning

confidence: 99%

Characterization of recombinant wild-type and nontoxigenic protein A from Staphylococcus pseudintermedius

2018

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Background: Staphylococcus pseudintermedius is an opportunistic pathogen that is the major cause of pyoderma affecting dogs. Conventional antimicrobial treatment for infections caused by this organism have failed in recent years due to widespread resistance and alternative treatment strategies are a high priority. Protein A encoded in Staphylococcus aureus by spa protects the bacterium by binding IgG and acts as a superantigen. Staphylococcus pseudintermedius possess two genes orthologous to S. aureus spa, spsP, and spsQ. Methods: SpsQ and SpsQ-M, a non-toxigenic SpsQ, were cloned and expressed as recombinant proteins and their cytotoxic effect on canine B cells was measured. The neutralizing ability of antibody raised against them in clinically healthy dogs was evaluated. Results: S. pseudintermedius SpsQ induced apoptosis of canine B cells. Specific amino acid substitutions diminished SpsQ-M binding to immunoglobulin and its super-antigenic activity, while its antigenicity was maintained. This recombinant, non-toxigenic S. pseudintermedius SpsQ stimulated the production of antibodies in dogs that specifically reacted with SpsQ and greatly diminished its cytotoxic effect on canine B cells. Conclusions: The production of neutralizing antibody suggests that attenuated, non-toxic SpsQ produced in this study is a good candidate for inclusion in a vaccine for use in the treatment and prevention of S. pseudintermedius infections.Abbreviations: SpA: Staphylococcus aureus protein A; SpsP: Staphylococcus pseudintermedius protein A; SpsQ: Staphylococcus pseudintermedius protein A; SpsQ-M: attenuated Staphylococcus pseudintermedius protein A; MRSP: methicillin resistant Staphylococcus pseudintermedius; IgA: immunoglobulin A; IgG: immunoglobulin G; IgM: immunoglobulin M; VH: variable region of immunoglobulin heavy chain; IgBD: immunoglobulin binding domains; MFI: mean fluorescent intensity; SEM: standard error of the mean; PBMC: Peripheral blood mononuclear cells; CD21: complement receptor type 2; ST: Sequence type; OD: Optical density; ORF: open reading frame; PBS: Phosphate buffered saline; Tween 20: Polyethylene glycol sorbitan monolaurate 20; HRP: horseradish peroxidase; TMB- 3,3',5,5'-Tetramethylbenzidine

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“…Recently, however, genotypic analyses revealed that it belongs to the Staphylococcus intermedius group (SIG), which comprises S. intermedius, S. pseudintermedius, and S. delphini [1,2]. It is the opportunistic bacteria that colonizes and clinically infects dogs and cats [3] and occasionally other animals [3,4], including humans [5]. Evidence of the zoonotic transmission of S. pseudintermedius from dogs to humans has been reported [5,6].…”

Section: Introductionmentioning

confidence: 99%

“…It is the opportunistic bacteria that colonizes and clinically infects dogs and cats [3] and occasionally other animals [3,4], including humans [5]. Evidence of the zoonotic transmission of S. pseudintermedius from dogs to humans has been reported [5,6].…”

Section: Introductionmentioning

confidence: 99%

“…pseudintermedius can cause skin infections such as pyoderma, external ear infections, wound infections, and abscesses, postoperative wound infections, and infections involving tissue and body cavities [5]. The microorganism more often colonizes the anal and nasal vestibular regions in healthy dogs than the groin, armpits, foreskin, vagina, external auditory canal, or interdigital skin [7,8].…”

Section: Introductionmentioning

confidence: 99%

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Occurrence of toxin genes in Staphylococcus pseudintermedius from diseased dogs and other domestic and wild species

Pitchenin

Brandão

Rosa

et al. 2018

J Infect Dev Ctries

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Introduction: Staphylococcus pseudintermedius is coagulase-positive species of the Staphylococcus intermedius group. It is an opportunistic pathogen that can cause infection in various parts of the body and has a zoonotic potential. Although studies on the pathogenicity and epidemiology of S. pseudintermedius are limited, it is known that this bacterium has several virulence factors, including toxins. These toxins can be classified into three main groups: pyrogenic toxins with superantigenic properties such as toxic shock syndrome toxin and staphylococcal enterotoxins, exfoliative toxins, and cytotoxins such as hemolysins and leukocidins. Methodology: In this study, the occurrence of eight toxin genes (sea, sec, tst, SIET, EXI, LuK F-I, Luk S-I, and hlg ƴ) was examined by PCR in 58 isolates of S. pseudintermedius from four domestic animal species. Results: All S. pseudintermedius isolates had at least one of the eight toxin genes. The predominant toxin genes were Luk S-I (95%), Luk F-I (91%), and EXI (91%), and the least prevalent gene was hlg ƴ (5%). Significant association (p = 0.0175) was found between the occurrence patterns of genes hlg ƴ and Luk F-I. Conclusions: The frequent occurrence of these genes in S. pseudintermedius obtained from diseased animals indicates that these toxins may play an important role in the pathogenesis of infection among domestic animals.

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Human infection associated with methicillin-resistant Staphylococcus pseudintermedius ST71

Cited by 160 publications

References 8 publications

Atomic force microscopy study of the antibacterial effect of fosfomycin on methicillin-resistant Staphylococcus pseudintermedius

Atomic force microscopy study of the antibacterial effect of fosfomycin on methicillin-resistant Staphylococcus pseudintermedius

Characterization of recombinant wild-type and nontoxigenic protein A from Staphylococcus pseudintermedius

Occurrence of toxin genes in Staphylococcus pseudintermedius from diseased dogs and other domestic and wild species

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