volume 66, issue 6, P3593-3601 1992
DOI: 10.1128/jvi.66.6.3593-3601.1992
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P A Sherman, M L Dickson, J A Fyfe

Abstract: Using purified integration protein (IN) from human immunodeficiency virus (HIV) type 1 and oligonucleotide mimics of viral and target DNA, we have investigated the DNA sequence specificity of the cleaving and joining reactions that take place during retroviral integration. The first reaction in this process is selective endonucleolytic cleaving of the viral DNA terminus that generates a recessed 3' OH group. This 3' OH group is then joined to a 5' phosphoryl group located at a break in the target DNA. We found…

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