Human Herpesvirus 8 Infection in Patients With Cutaneous Lymphoproliferative Diseases

High Association of Human Herpesvirus 8 in Large-Plaque Parapsoriasis and Mycosis Fungoides

“…The presence of HHV-8 DNA was analyzed by amplification of a sequence of open reading frame (ORF) 26 by a nested polymerase chain reaction (PCR) protocol similar to the protocol reported by Trento et al 7 Identical PCR primers were used. 7 To prevent possible cross-contamination between samples during the PCR procedure, each paraffin block was cut using a new disposable microtome blade.…”

“…7 To prevent possible cross-contamination between samples during the PCR procedure, each paraffin block was cut using a new disposable microtome blade. For DNA extraction, tissue samples were deparaffinized by adding 1 mL of xylene.…”

“…4 Some years ago, several studies provided strong evidence against a role for HHV-8 in the pathogenesis of mycosis fungoides (MF), the most common subtype of cutaneous T-cell lymphoma (CTCL). 5,6 In the October 2005 issue of the Archives, Trento and colleagues 7 investigated the prevalence of HHV-8 infection in an Italian population of 22 patients with lymphoproliferative diseases such as largeplaque parapsoriasis (LPP) and MF. Lesional skin samples, plasma, and peripheral blood mononuclear cells were analyzed for the presence of HHV-8.…”

Cutaneous lymphoma incidence patterns in the United States: a population-based study of 3884 cases

“…1 Risk factors for MF include advanced age, black race, and male sex. 4 Although infectious agents [9][10][11] and environmental exposures 12 have been studied, the etiology of MF remains unknown. The CD30 ϩ lymphoproliferative disorders are characterized by expression of the cell-surface receptor CD30 ϩ , which is a marker of activated T cells and a member of the tumor necrosis factor superfamily.…”

Avaliação da resposta tecidual "in situ" do fenótipo, da expressão de HHV-8/LANA e de citocinas em lesões cutâneas de sarcoma de Kaposi clássico e sarcoma de Kaposi associado à AIDS na era pré e pós-terapia anti-retroviral combinada

“…Mais recentemente, alguns estudiosos sugeriram a participação desse herpesvírus na patogênese do mieloma múltiplo, da parapsoríase e da hipertensão pulmonar, porém, são estudos ainda não conclusivos (Trento et al, 2005). A associação do HHV-8 com outros linfomas não Hodgkin apresentam controvérsias, havendo a necessidade de mais estudos (Schulz, 1999;Carbone et al, 2008).…”