Human HepG2 cells support respiratory syncytial virus and human metapneumovirus replication

Schildgen, Verena; Lüsebrink, Jessica; Ditt, Vanessa; Tillmann, Ramona Liza; Simon, Arne; Müller, Andreas; Schildgen, Oliver

doi:10.1016/j.jviromet.2009.08.024

Cited by 11 publications

(11 citation statements)

References 37 publications

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“…For calibration purposes and as a quality control for each test, serial virus dilutions were run in parallel on each plate to ensure that the virus concentration in the inoculum was reciprocal to cell viability, as previously shown (17). As expected, the absorption rate increased with decreasing virus concentrations (Fig.…”

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confidence: 58%

“…Serum samples from a total of 2,000 patient were randomly collected from the archives of the Institute of Virology of the University Hospital Bonn (which includes a large trauma center for the geographic area and a large obstetrics unit, resulting in many patients in the 20-to 50-year-old age range) and screened for neutralizing capacity, using the XTT-based neutralization test described previously (17).…”

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confidence: 99%

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High Seroprevalence of Neutralizing Capacity against Human Metapneumovirus in All Age Groups Studied in Bonn, Germany

Lüsebrink

Wiese

Thiel

et al. 2010

Clin Vaccine Immunol

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Human metapneumovirus (hMPV) infections occur frequently despite high rates of perpetual seroprevalence for all age groups. Analyses of ϳ2,000 archived, randomly selected serum samples demonstrated that neutralizing capacities remain high, with a minor decrease for individuals over 69 years of age, leading to the hypothesis that reinfections occur because humoral immune responses play minor roles in the clearance of hMPV infections.

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confidence: 58%

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confidence: 99%

High Seroprevalence of Neutralizing Capacity against Human Metapneumovirus in All Age Groups Studied in Bonn, Germany

Lüsebrink

Wiese

Thiel

et al. 2010

Clin Vaccine Immunol

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“…HMPV can be cultured in several cell lines, including tertiary monkey kidney cells (231), Vero cells (231), LLC-MK2-cells, BEAS-2B cells (226), A549 cells (14), and HepG2 cells (202). These cell culture models facilitate HMPV research; however, there are caveats related to the different envelopes the viruses acquire as they bud from the cells, and these different envelopes may modify immune responses and may interfere with some assays.…”

Section: Cell Culturementioning

confidence: 99%

Human Metapneumovirus: Lessons Learned over the First Decade

Schildgen

Hoogen²,

Fouchier³

et al. 2011

Clin Microbiol Rev

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SUMMARY It has been 10 years since human metapneumovirus (HMPV) was identified as a causative agent of respiratory illness in humans. Since then, numerous studies have contributed to a substantial body of knowledge on many aspects of HMPV. This review summarizes our current knowledge on HMPV, HMPV disease pathogenesis, and disease intervention strategies and identifies a number of areas with key questions to be addressed in the future.

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“…6,14 Other cells (Hep2 and HepG2) can support the replication of some hMPV strains. 15,16 Its growth in cell culture is difficult and long and cytopathic effects (CPEs) are variable. The hMPV-induced CPE is characterized by a rounding of infected cells, subsequent detachment from cell culture matrix and occasionally the presence of small syncytia which do not usually appear before the second week of culture in LLC-MK2 cells.…”

Section: Diagnosismentioning

confidence: 99%