Human Gut Microbiome Response Induced by Fermented Dairy Product Intake in Healthy Volunteers

Volokh, Olesya; Klimenko, Natalia; Berezhnaya, Yulia; Tyakht, Alexander; Nesterova, Polina; Popenko, Anna; Alexeev, Dmitry

doi:10.3390/nu11030547

Cited by 59 publications

(36 citation statements)

References 48 publications

(51 reference statements)

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“…The relative abundance of Collinsella was increased in cultures with bovine milk, and this observation matched in vivo results observed in an animal study (Rettedal et al, 2019). We also observed increased proportions Catenibacterium in cultures with milk as a substrate, which align with results from a recent study where consumption of a fermented dairy product also increased Catenibacterium abundance in human volunteers (Volokh et al, 2019). Bacteroides were also increased in the cultures with milk over time, as they were with all insect substrates.…”

Section: Discussionsupporting

confidence: 90%

Feeding Bugs to Bugs: Edible Insects Modify the Human Gut Microbiome in an in vitro Fermentation Model

et al. 2020

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Section: Discussionsupporting

confidence: 90%

Feeding Bugs to Bugs: Edible Insects Modify the Human Gut Microbiome in an in vitro Fermentation Model

et al. 2020

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“…As a major feature, there is a strong enrichment in microbes associated with inflammation-Enterobacteriaceae, Enterococcus, Streptococcus, and Staphylococcus, coupled with a depletion of commensal microbes. Their relative abundance does not typically exceed 1% in healthy subjects (as estimated in the same population using similar approaches) [29][30][31].…”

Section: Gut Taxonomy Dysbiosis In Critically Ill Patients and Healthmentioning

confidence: 85%

Serum and fecal profiles of aromatic microbial metabolites reflect gut microbiota disruption in critically ill patients: a prospective observational pilot study

et al. 2020

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Background: High serum levels of certain aromatic microbial metabolites (AMM) are associated with severity and mortality in critically ill patients. Omics-based studies suggest gut dysbiosis and reduced microbiome diversity in critical conditions. However, the landscape of gut microbial metabolites is still to be outlined, not to mention the interplay correlation between the metabolome and gut microbiome in critically ill patients. The aim of this study was to analyze the association between serum and fecal levels of AMM and compare them with the composition of gut microbiota in critically ill patients in the acute and chronic stages. Methods: In this prospective observational pilot study, we analyzed the temporal dynamics of the gut microbiome and the AMM spectrum across two distinct subgroups-acute critical ill (ACI) patients with nosocomial pneumonia and chronically critically ill (CCI) patients (9 subjects each group)-as well as performed comparison with 23 healthy volunteers. The AMM levels for each patient were measured using GC-MS in simultaneously taken serum and fecal samples (SFS). These parameters were compared with 16S rRNA fecal microbiome profiles. Results: The observed proportions of bacterial taxa suggest a significant gut dysbiosis in the ACI and the CCI patients. Stronger imbalance in microbiome composition and dynamics observed in the ACI patients compared to the CCI ones resonates with a higher severity in the former group. The total levels of AMM in serum samples were higher for the ACI patients than for the CCI patients (3.7 (1.4-6.3) and 1.1 (1.0-1.6) μM, respectively; p = 0.0003). The qualitative composition of the SFS was also altered. We discovered significant associations between gut microbial taxa levels and metabolite concentrations in blood serum as well as in feces in each of the ACI and the CCI patients. Conclusions: Aromatic microbial metabolite profiles in the gut and the serum are interlinked and reflect a disruption of the gut microbial community in critically ill patients.

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“…DNA extraction from rat fecal samples and library preparation was performed, as described in [94]. The V4 region of the 16S rRNA gene was amplified using the following modification of 515F-806R primers: GTGBCAGCMGCCGCGGTAA and GACTACNVGGGTMTCTAATCC.…”

Section: Dna Extraction and Sequencingmentioning

confidence: 99%

Microbiome-Metabolome Signature of Acute Kidney Injury

et al. 2020

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Intestinal microbiota play a considerable role in the host’s organism, broadly affecting its organs and tissues. The kidney can also be the target of the microbiome and its metabolites (especially short-chain fatty acids), which can influence renal tissue, both by direct action and through modulation of the immune response. This impact is crucial, especially during kidney injury, because the modulation of inflammation or reparative processes could affect the severity of the resulting damage or recovery of kidney function. In this study, we compared the composition of rat gut microbiota with its outcome, in experimental acute ischemic kidney injury and named the bacterial taxa that play putatively negative or positive roles in the progression of ischemic kidney injury. We investigated the link between serum creatinine, urea, and a number of metabolites (acylcarnitines and amino acids), and the relative abundance of various bacterial taxa in rat feces. Our analysis revealed an increase in levels of 32 acylcarnitines in serum, after renal ischemia/reperfusion and correlation with creatinine and urea, while levels of three amino acids (tyrosine, tryptophan, and proline) had decreased. We detected associations between bacterial abundance and metabolite levels, using a compositionality-aware approach—Rothia and Staphylococcus levels were positively associated with creatinine and urea levels, respectively. Our findings indicate that the gut microbial community contains specific members whose presence might ameliorate or, on the contrary, aggravate ischemic kidney injury. These bacterial taxa could present perspective targets for therapeutical interventions in kidney pathologies, including acute kidney injury.

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Human Gut Microbiome Response Induced by Fermented Dairy Product Intake in Healthy Volunteers

Cited by 59 publications

References 48 publications

Feeding Bugs to Bugs: Edible Insects Modify the Human Gut Microbiome in an in vitro Fermentation Model

Feeding Bugs to Bugs: Edible Insects Modify the Human Gut Microbiome in an in vitro Fermentation Model

Serum and fecal profiles of aromatic microbial metabolites reflect gut microbiota disruption in critically ill patients: a prospective observational pilot study

Microbiome-Metabolome Signature of Acute Kidney Injury

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